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Epitope Tag Antibody | Protein Research

Protein Research

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Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

  • Immunohistochemical application
  • Rat monoclonal antibody

Immunocytochemistry


Sample : Mouse brain (nerve cell)
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (1:1000) RT, O/N
Secondary antibody : Anti-Rat IgG-Cy3 (1:300) RT, 1hr
Fixing method : 4% Paraformaldehyde
Blocking : Data courtesy of Dr.Yoshihara Y., RIKEN Brain Science Institute

Clone : GF090R
Isotype : IgG2a(Rat)
Product form : Liquid
Immunogen : His-GFP (full length) fusion protein
Application : Immunohistochemstry 1:1000 - 1:2000
Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000

References

  1. Nakashiba T et al. Science 319(5867), 1260-1264 (2008)
  2. Batista-Brito R et al. The Journal of Neuroscience 28(15), 3966-3975 (2008)
  3. Esumi S et al. Neuroscience Research 60(4), 439-451 (2008)
  4. Fogarty M et al. The Journal of Neuroscience 27(41), 10935-10946 (2007)
  5. Sasamura T et al. Development 134, 1347-1356 (2007)
  6. Sato Y et al. The Journal of Neuroscience 27(7), 1606-1615 (2007)
  7. Yamada M et al. Stem Cells 25(3), 562-570 (2007)
  8. Togashi H et al. The Journal of Cell Biology 174, 141-151 (2006)
  9. Ogata M. et al. Molecular and Cell Biology 26(24), 9220-9231 (2006)
  10. Sato Y et al. The Journal of Neuroscience 25(20), 4889-4897 (2005)

Ordering information

Product Storage Cat.No. Quantity Price(US$)  
Anti-GFP (Rat IgG2a), Monoclonal (GF090R) 2-8 °C 04404-84 200 ug 390 Buy Now

Anti-GFP (Mouse IgG1-κ), Monoclonal (GF200)

Western blotting


Sample :
lane 1) : HeLa:GFP-H2A-Bbd
lane 2) : HeLa:GFP-H2B
lane 3) : HeLa
Data courtesy of Dr. Kimura H., Horizontal Medical Research Organization, Kyoto University Faculty of Medicine


Data courtesy of Dr. Shimada T., Department of Botany, Graduate School of Science, Kyoto University 		Protein extract from seedlings of Arabidopsis (10mg)
Lane1 : Non-transformants (Control)
Lane2 : Transformants

Primary antibody:
Anti-GFP(mouse IgG-κ) (1:5000)
Secondary antibody:
Anti-mouse IgG-HRP (1:2000)

Clone : GF200
Isotype : IgG1-κ(Mouse)
Product form : Liquid
Immunogen : His-GFP (full length) fusion protein
Application : Western blotting 1:1000 - 1:2000
ELISA 1:2000 - 1:20000

Ordering information

Product Storage Cat.No. Quantity Price(US$)  
Anti-GFP (Mouse IgG1-k), Monoclonal (GF200) 2-8 °C 04363-24 200 ug 390 Buy Now

Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045)

Immunocytochemistry


Sample : SH-SY5Y cell in which c-Myc-tagged TCP1β is expressed
Primary antibody : Anti-c-Myc (Mouse IgG1-κ) (2.5mg/ml) Anti-Protein X
Secondary antibody : Alexa Fluor 546-conjugated antibody (1:400)
Alexa Fluor 488-conjugated antibody (1:400)
Data courtesy of RIKEN Brain Science Institute


Sample : HeLa cell in which c-Myc-tagged(N-terminal) hnRNP A1 is expressed
Primary antibody : Anti-c-Myc (Mouse IgG1-κ) (1:1000)
Secondary antibody : FITC-conjugated goat affinity purified f(ab')2 fragment to mouse IgG (whole molecule) (1:200)
Permeabilization : Acetone (-20°C, 3min)
Blocking : 3% BSA in PBS (RT)
Data courtesy of Dr. Kataoka N., Department of Genetics and Molecular Biology, Laboratry of Biochemistry, Institute for Virus Research, Kyoto University.

Clone : MC045
Isotype : IgG1-κ(mouse)
Product form : Liquid
Immunogen : c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH
Application : Western blotting 1:1000 - 1:2000
Immunoprecipitation 1:400 - 1:1000
Immunocytochemistry 1:100 - 1:200
ELISA 1:2000 - 1:20000

Ordering information

Product Storage Cat.No. Quantity Price(US$)  
Anti-c-Myc (Mouse IgG1-k), Monoclonal (MC045) 2-8 °C 04362-34 200 ug 390 Buy Now
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