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Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

Features

  • Immunohistochemical application
  • Rat monoclonal antibody

Anti-GFP (Rat IgG2a), Monoclonal (GF090R)

Clone : GF090R
Isotype : IgG2a (Rat)
Product form : Liquid
Immunogen : His-GFP (full length) fusion protein
Application : Immunohistochemistry 1:1000-1:2000
Western Blotting 1:1000-1:2000
ELISA 1:2000-1:20000

Anti-GFP (Rat IgG2a), Monoclonal (GF090R), Agarose Conjugate

Antibody protein : ca. 2 mg antibody / ml agarose
Product form : 1:1 suspension in Dulbecco's PBS(-)

Immunocytochemistry

Sample : Mouse brain (nerve cell)
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (1:1000), RT, O/N
Secondary antibody : Anti-Rat IgG-Cy3 (1:300), RT, 1hr
Blocking : 5% Normal goat serum/0.2% TritonX-100 in PBS
Fixing method : 4% Paraformaldehyde

Data courtesy of Dr. Y. Yoshihara, RIKEN Brain Science Institute

Reference
  1. Yonehara, K. Nature. 469(7330):407-10 (2011)
  2. Abe, K. et al. Biol Reprod 85(5):1013-24 (2011)
  3. Hide, T. et al. Stem Cells. 29(4):590-9 (2011)
  4. Kurita, S. et al. J Biol Chem. 286(42):36297-303 (2011)
  5. Wang, X. et al. Glia. 59(6):857-68 (2011)
  6. Mochizuki, N. Biol Pharm Bull. 34(2):260-5 (2011)
  7. Inamura, N. et al. Dev Neurosci. 33(2):118-29 (2011)
  8. Vidaki, M. et al. Cereb Cortex. (2011)
  9. Konno, A. et al. Neurosci Res. (2011)
  10. Gotoh, H. et al. Dev Biol. 349(2):504-11 (2011)
  11. Mains, RE. et al. BMC Neurosci. 12:20 (2011)
  12. Rubin, AN. et al. J Neurosci. 30(36):12050-62 (2010)
  13. Matsumoto, H. et al. J Neurophysiol. 103(6):3490-500 (2010)
  14. Tamura, H. et al. J Histochem Cytochem. 58(5):443-53 (2010)
  15. Asai, R. et al. Development. 137(22):3823-33 (2010)
  16. Hirota, Y. et al. Development. 137(18):3037-46 (2010)
  17. Honma, Y. et al. Development. 137(14):2319-28 (2010)
  18. Kohyama, J. J Cell Biol. 189(1):159-70 (2010)
  19. Manno, A. et al. Genes Cells. 15(8):911-22 (2010)
  20. Nakamura, M. et al. Molecular Vision 16, 425-437 (2010)
  21. Nishide, K. et al. PLoS ONE 4(8), e6869 (2009)
  22. Nagano, M. et al. The Journal of Cell Biology 183(7):1243-1257 (2008)
  23. Ono, K. et al. Development Biology 320(2):356-468 (2008)
  24. Nakashiba T. et al. Science 319(5867):1260-1264 (2008)
  25. Batista-Brito R. et al. The Journal of Neuroscience 28(15): 3966-3975 (2008)
  26. Esumi S. et al. Neuroscience Research 60(4):439-451 (2008)
  27. Fogarty M. et al. The Journal of Neuroscience 27(41):10935-10946 (2007)
  28. Sasamura T. et al. Development 134:1347-1356 (2007)
  29. Sato Y. et al. The Journal of Neuroscience 27(7):1606-1615 (2007)
  30. Yamada M. et al. Stem Cells 25(3):562-570 (2007)
  31. Togashi H. et al. The Journal of Cell Biology 174:141-151 (2006)
  32. Ogata M. et al. Molecular and Cell Biology 26(24):9220-9231 (2006)
  33. Sato Y. et al. The Journal of Neuroscience 25(20):4889-4897 (2005)

Western Blotting

Sample : 1. GFP, 2. GFP-14-3-3 (293 cell lysate 50 µg)
Gel : 5-20% Gradient gel
Primary antibody : Anti-GFP(Rat IgG2a), Monoclonal (3 µg/ml), O/N
Secondary antibody : Anti-Rat IgG-HRP (1:10000)

Data courtesy of Dr. K. Yoshida, Depertment of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University.

Immunoprecipitation : Anti-GFP (Rat IgG2a), Agarose Conjugate

Protocol

  1. Add Anti-GFP (Rat IgG2a), agarose conjugate (20 µl) in 293 Cell Lysate (1 ml) expressed EGFP or EGFP-myosin (1ml each). Incubate at 4°C for 1 hour.
  2. Add SDS Sample buffer. After heating the samples at 90°C for 10 minutes, load to SDS-PAGE.
  3. Detect EGFP and EGFP-myosin using Anti-GFP (Rat IgG2a).

Data courtesy of Prof. A. Kakizuka, Laboratory of Functional Biology, Kyoto University Graduate School of Biostudies.

Reference
  1. Kumeta, M. et al. J Cell Sci. 123(Pt 7):1020-30 (2010)

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Anti-GFP (Rat IgG2a), Monoclonal (GF090R) 04404-84 2-8 °C 200 µg 390.00 Buy
Anti-GFP (Rat IgG2a), Monoclonal (GF090R), Agarose Conjugate 06083-05 2-8 °C 500 µg 460.00 Buy