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Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045)

Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045)

Clone : MC045
Isotype : IgG1-κ (Mouse)
Product form : Liquid
Immunogen : c-Myc synthetic peptide [EQKLISEEDL] conjugated with KLH
Application : Western Blotting 1:1000-1:2000
Immunoprecipitation 1:400-1:1000
Immunocytochemistry 1:100-1:200
ELISA 1:2000-1:20000

Anti-c-Myc (Mouse IgG1-κ), Monoclonal (MC045), Agarose Conjugate

Antibody protein : ca. 2mg antibody / ml agarose
Product form : 1:1 suspension in Dulbecco's PBS(-)

Immunocytochemistry

Application 1

Sample : SH-SY5Y cell in which c-Myc-tagged TCP1β is expressed
Primary antibody : Anti-c-Myc (Mouse IgG1-κ) (2.5mg/ml) Anti-Protein X
Secondary antibody : Alexa Fluor 546-conjugated antibody (1:400)
Alexa Fluor 488-conjugated antibody (1:400)

Data courtesy of RIKEN Brain Science Institute

Application 2

Sample : HeLa cell in which c-Myc-tagged(N-terminal) hnRNP A1 is expressed
Primary antibody : Anti-c-Myc (Mouse IgG1-κ) (1:1000)
Secondary antibody : FITC-conjugated goat affinity purified f(ab')2 fragment to mouse IgG (whole molecule) (1:200)
Permeabilization : Acetone (-20°C, 3min)
Blocking : 3% BSA in PBS (RT)

Data courtesy of Dr. Kataoka N., Department of Genetics and Molecular Biology, Laboratry of Biochemistry, Institute for Virus Research, Kyoto University.

Immunoprecipitation: Anti-c-Myc (Mouse IgG1-k), Agarose Conjugate

Sample : c-Myc fusion protein
Immunoprecipitation : Anti-c-Myc, (Mouse IgG1-κ), Agarose Conjugate (1:500)
Western Blotting : Anti-c-Myc, (Mouse IgG1-κ)

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Anti-c-Myc (Mouse IgG1-k), Monoclonal (MC045) 04362-34 2-8 °C 200 µg 260.00 Buy
Anti-c-Myc (Mouse IgG1-k), Monoclonal (MC045), Agarose Conjugate 04145-55 2-8 °C 500 µg 300.00 Buy