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Signal Enhancer HIKARI

Signal Enhancer for Western Blotting and ELISA

Dilute your antibodies with Signal Enhancer HIKARI instead of conventional diluents such as PBS or TBS before performing your next Western blot detection protocol and witness a remarkable increase in the ability to detect the protein of interest and to eliminate undesired background.
Signal Enhancer HIKARI was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA.

Features

  • Enhances antigen-antibody reactions - yields several 10-fold increase in signal intensity
  • Removes background - improves the specificity of your antibodies
  • Works with any substrate - enhances both chemiluminescence and colorimetric detection
  • Works with any membrane - enhances signal from nitrocellulose and PVDF membrane
  • Ready-to-use reagent - just dilutes your antibodies with Signal Enhancer HIKARI

Application 1

Detection enhancement of Rac1 with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

Primary Ab: Anti-Rac1 (1: 1,000)
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500)
Sample: Rat cortical primary neuron
Detection: ECL

Data courtesy of Dr. K. Ishizuka,
Department of Psychiatry,Johns Hopkins University School of Medicine

Application 2

Detection enhancement of pJNK with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T.

Primary Ab: Anti-pJNK (1: 1,000)
Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000)
Sample: Mouse Embryonic Fibroblast (MEF)
Detection: SuperSignal West Pico

Data Courtesy of Dr. S. Matsuzawa,
Signal Transduction, NCI Cancer Center, Burnham Institute for Medical Research

Technical Information

Signal Enhancer HIKARI Procedure (PDF)

MSDS

Signal Enhancer HIKARI (PDF)

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Signal Enhancer HIKARI 50 (Solution 1* 50 ml, Solution 2** 50 ml) NU00101 4°C 1 Kit 98.00 Buy
Signal Enhancer HIKARI 250 (Solution 1* 250 ml, Solution 2** 250 ml) NU00102 4°C 1 Kit 280.00 Buy
Signal Enhancer HIKARI - Solution 1 NU00102-1 4°C 250 ml 160.00 Buy
Signal Enhancer HIKARI - Solution 2 NU00102-2 4°C 250 ml 160.00 Buy

* Solution 1 for Primary Antibody
** Solution 2 for Secondary Antibody