Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. The recombinant Turbo3C Protease is a restriction grade HRV3C protease that has robust activity at 4°C and high specific activity. Turbo3C Protease is a 47 kDa protein with both GST and His tags so it can be removed by either Ni chelating or Glutathione (GSH) resin.
A 68 kDa GST-fusion protein (C) at 1 mg/ml is incubated with Turbo3C Protease (*) at a ratio of (1) 1:50, (2) 1:100, (3) 1:200, (4) 1:400 (w/w) in a buffer of 25 mM Tris-HCl, pH8.0, 150 mM NaCl, 14 mM 2-mercaptoethanol at 4°C for 16 hours. The cleaved products are 42 kDa and 26 kDa