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COSMOSIL Protein-R

Reversed Phase / For protein and peptide separation

COSMOSIL Protein-R is a reversed phase HPLC column designed specifically for protein and peptide separation. COSMOSIL Protein-R provides significantly improved peak shapes, high recovery rate and outstanding stability at low pH, which are often problematic for the separation of proteins and peptides with conventional C18-300Å and C4-300Å columns.

COSMOSIL Protein-R
Silica gel high purity spherical silica
Average particle size 5µm
Average pore size approx. 300Å
Specific surface area approx. 150m2/g
Stationary phase octadecyl group
Bonding type polymeric

 

Application

Excellent Separation
COSMOSIL Protein-R, C18-300Å (Competitor A), C4-300Å (Competitor B)
Column size: 4.6 mm I.D. x 150 mm   Sample: 1. Ribonuclease A (1.0 µg)
Mobile phase: A: 0.05% TFA - 20% Acetonitrile     2. Cytochrome C (1.0 µg)
  B: 0.05% TFA - 60% Acetonitrile     3. Lysozyme (1.0 µg)
  B: 0 → 100% 20 min linear gradient     4. BSA (1.0 µg)
Flow rate: 1.0 ml/min     5. Myoglobin (1.0 µg)
Temperature: 30°C     6. Ovalbumin (1.0 µg)
Detection: UV 220 nm      

High Recovery Rate
Protein-R shows a higher recovery rate than conventional C18-300Å and C4-300Å columns.

Protein-R, C18-300Å (Competitor A), C4-300Å(Competitor B)

Outstanding Stability at Low pH

Protein-R provides more consistent retention time and longer column lifetimes than conventional C4-300Å

Degradation test with 0.1 % TFA solution at 60°C

Ordering Information

Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL Protein-R Guard Column, 4.6 mm I.D. x 10 mm 06518-31 Waters type 1 pkg 220.00 Buy
COSMOSIL Protein-R Guard Column, 10.0 mm I.D. x 20 mm 06528-01 Waters type 1 pkg 490.00 Buy
COSMOSIL Protein-R Guard Column, 20.0 mm I.D. x 20 mm 08692-81 Waters type 1 pkg 1,040.00 Buy
COSMOSIL Protein-R Packed Column, 2.0 mm I.D. x 150 mm 06514-71 Waters type 1 pkg 460.00 Buy
COSMOSIL Protein-R Packed Column, 4.6 mm I.D. x 50 mm 06525-31 Waters type 1 pkg 390.00 Buy
COSMOSIL Protein-R Packed Column, 4.6 mm I.D. x 150 mm 06526-21 Waters type 1 pkg 480.00 Buy
COSMOSIL Protein-R Packed Column, 4.6 mm I.D. x 250 mm 06527-11 Waters type 1 pkg 560.00 Buy
COSMOSIL Protein-R Packed Column, 10.0 mm I.D. x 150 mm 06529-91 Waters type 1 pkg 1,040.00 Buy
COSMOSIL Protein-R Packed Column, 10.0 mm I.D. x 250 mm 06530-51 Waters type 1 pkg 1,320.00 Buy
COSMOSIL Protein-R Packed Column, 20.0 mm I.D. x 150 mm 06531-41 Waters type 1 pkg 2,240.00 Buy
COSMOSIL Protein-R Packed Column, 20.0 mm I.D. x 250 mm 06532-31 Waters type 1 pkg 2,930.00 Buy