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Total RNA Purification Kit

For purification (spin column) of total RNA

Norgen's Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA in as little as 20 minutes. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), without the use of phenol or chloroform. Purification is based on spin column chromatography using Norgen's proprietary resin as the separation matrix.

Features

  • No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
  • Isolate total RNA from very small samples - Total RNA has been isolated and detected from as little as a single animal cell
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
  • Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes
  • Isolate total RNA from a broad input source - Total RNA has been isolated from cultured animal cells, small tissue samples, LCM samples, bacteria, yeast, fungi, plants, viruses and various bodily fluid including blood, plasma, serum, saliva, nasal or throat swabs
  • No need for carrier RNA - Isolate all sizes of RNA without the use of carrier RNA

Application

  • Quantitative, real-time RT-PCR for both large mRNA and small RNA including miRNA
  • RT-PCR for both large mRNA and small RNA including miRNA
  • Expression profiling
  • Next Generation Sequencing for RNA and miRNA
  • miRNA from plasma for discovery
  • miRNA cloning and amplification
  • PCR-based virus detection
  • PCR-based viable bacteria detection
  • Northern blotting
  • RNase protection
  • Primer extension

Figure 1. High Quality of Isolated RNA with Complete Size Range.

Unlike most competitor's kits, Norgen's Total RNA Purification Kit allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA without the use of phenol. Total RNA was isolated from 1 x 109 E. coli cells using Norgen's Total RNA Purification Kit and a competitor's kit. Five microliters and 1 µL of the 50 µL isolated RNA was analyzed on an agarose gel (Panel A) and the Agilent® 2100 BioAnalyzer RNA Nano 6000 chip (Panel B), respectively. Note the presence of small RNA species (red circle) in the samples isolated via Norgen's kit and the absence of these RNA species in the competitor RNA preparation.

Small RNA Species

Figure 2: Better Diversity of miRNA Detected from Plasma.

Norgen's Total RNA Purification Kit isolates miRNA from plasma with better diversity than a leading competitor. Total RNA including miRNA was isolated from 100 µL of plasma using Norgen's Total RNA Purification Kit or 625 µL of plasma using Competitor A's leading miRNA Kit, and was applied to an NCode expression profiling kit. Microarray images suggested that Norgen's Total RNA Purification Kit (left) isolates a better diversity of miRNA from smaller input amount of plasma than the competitor's miRNA kit (right). Image courtesy of LC Sciences, Houston.

Kit Specifications

Binding Capacity Per Column Up to 50 µg RNA
Maximum Loading Volume Per Spin Column 650 µL
Size of RNA Purified All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications 20 minutes

RNA Yield

Liver (10 mg) 30 µg
Kidney (10 mg) 10 µg
Brain (10 mg) 12 µg
Blood (hamster, 100 µL) 5 µg
HeLa (1 x 106) 15 µg
CHO (1 x 106) 11 µg
Yeast (1 x 108) 30 µg
E coli (1 x 109) 43 µg

Total RNA Purification Kit Contents

  1. Spin Column
  2. Lysis Solution
  3. Wash Solution
  4. Elution Buffer
  5. Micro Spin Columns
  6. Collection Tubes
  7. Elution Tubes
  8. Product Insert

Storage Conditions and Product Stability

All Solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers

Protocol

Total RNA Purification Kit (PDF)

MSDS

Total RNA Purification Kit (PDF)

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Total RNA Purification Kit 17200 Room Temp. 50 preps 233.00 Buy
Total RNA Purification Kit 37500 Room Temp. 100 preps 447.00 Buy

* Total RNA Purification Kit is produced by Norgen Biotek in CANADA.