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Recombinant Human FGF-basic, Animal-free

Recombinant human FGF-basic (bFGF) (AA 1-154), also called as FGF-2 or FGFb, is a bioactive protein intended for use in cell culture applications. bFGF is a heparin-binding member of the FGF superfamily of molecules. It is involved in a number of biological processes including embryonic development, differentiation, survival, regeneration and migration. In addition, bFGF is a critical factor for growing embryonic stem cells in culture to remain cell in an undifferentiated state.

Cell Culture of NIH/3T3 Cells


NIH/3T3 cells (mouse fibroblast cell line, ATCC: CCL-1668) were cultured in the absence of bFGF.

NIH/3T3 cells were cultured with 0.25 ng/ml bFGF in serum-free medium at 37°C, 5% CO2 for 24 hours.

Bioactivity assay

Human FGF basic recombinant protein bioactivity measured by mouse BALB/c 3T3 cell proliferation assay. The cell proliferation dose curves were plotted with mean OD vs. bFGF concentrations (ng/mL).

SDS-PAGE of human bFGF

Figure 1

Source

Escherichia Coli.

Purity

Greater than 98%, as determined by SDS-PAGE (See Figure 1)

Endotoxin

Less than 0.01 ng/ug cytokine as determined by the LAL assay

Bioactivity

ED50= 0.1-0.3 ng/ml as determined by the dose dependent proliferation of NIH 3T3 cells.

Formulation

Sterile filtered and lyophilized from Tris buffer (10 mM Tris pH7.5, 150 mM NaCl).

Storage

bFGF is shipped at 4°C with ice packs. Lyophilized bFGF is stable at -20°C for up to 12 months from date of receipt.

Reconstitution

Centrifuge vial before opening. It is recommended to reconstitute bFGF in sterile 10 mM Tris-HCl, pH7.5 to yield a stock solution of 0.1 mg/ml of bFGF. It is stable for up to 6 months when stored at -20°C and up to 12 months when stored at -80°C. Multiple freeze/thaw cycles will result in significant loss of activity.

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Recombinant Human FGF-basic, Animal-free NU0005-1 -20°C 10 µg 80.00 Buy
Recombinant Human FGF-basic, Animal-free NU0005-3 -20°C 50 µg 180.00 Buy
Recombinant Human FGF-basic, Animal-free NU0005-6 -20°C 1 mg 1,000.00 Buy