Recombinant human FGF-basic (bFGF) (AA 1-154), also called as FGF-2 or FGFb, is a bioactive protein intended for use in cell culture applications. bFGF is a heparin-binding member of the FGF superfamily of molecules. It is involved in a number of biological processes including embryonic development, differentiation, survival, regeneration and migration. In addition, bFGF is a critical factor for growing embryonic stem cells in culture to remain cell in an undifferentiated state.
|Purity:||Greater than 98%, as determined by SDS-PAGE (See SDS-PAGE Image)|
|Endotoxin:||Less than 0.01 ng/ug cytokine as determined by the LAL assay|
|Bioactivity:||ED50= 0.1-0.3 ng/ml as determined by the dose dependent proliferation of NIH 3T3 cells.|
|Formulation:||Sterile filtered and lyophilized from Tris buffer (10 mM Tris pH7.5, 150 mM NaCl).|
|Storage:||bFGF is shipped at 4°C with ice packs. Lyophilized bFGF is stable at -20°C for up to 12 months from date of receipt.|
|Reconstitution:||Centrifuge vial before opening. It is recommended to reconstitute bFGF in sterile 10 mM Tris-HCl, pH7.5 to yield a stock solution of 0.1 mg/ml of bFGF. It is stable for up to 6 months when stored at -20°C and up to 12 months when stored at -80°C. Multiple freeze/thaw cycles will result in significant loss of activity.|
NIH/3T3 cells (mouse fibroblast cell line, ATCC: CCL-1668) were cultured in the absence of bFGF.
NIH/3T3 cells were cultured with 0.25 ng/ml bFGF in serum-free medium at 37°C, 5% CO2 for 24 hours.
Human FGF basic recombinant protein bioactivity measured by mouse BALB/c 3T3 cell proliferation assay. The cell proliferation dose curves were plotted with mean OD vs. bFGF concentrations (ng/mL).