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C18 / C8

SunShell HPLC Columns

SunShell HPLC columns are manufactured using innovative core shell particle technology that provides significantly increased column efficiency and chromatographic resolution without the high back pressure seen in sub-2 µm columns.

Features

- 2.6 µm core shell particle with 0.5 µm porous silica layer
- Provides the same high efficiency and high speed as sub-2 µm
- Less than half the backpressure of sub-2 µm
- Up to 100 times higher loading capacity compared to other core shell materials
- Improved pH stability from 1.0 to 10 (C18)

SunShell HPLC Columns are manufactured by ChromaNik Technologies, Inc.

Physical Characteristics

 Stationary PhaseParticle SizePore SizeSurface AreaCarbon ContentpH Range
C18 C18 2.6 µm 90Å 150 m²/g Approx. 7% 1.5 - 10
C8 C8 2.6 µm 90Å 150 m²/g Approx. 4.5% 1.5 - 9

 

Schematic of Diffusion Paths

2.6 µm core shell particle provides the same diffusion width as 2.0 µm totally porous particle.

Diffusion of  sample in core shell and totally porous silica

Comparison of Plate Height Plots

SunShell C18 column shows the same efficiency as sub-2 µm C18 column.

Column: C18, 50 x 4.6 mm C18, Mobile phase: Acetonitrile/water=(60/40), Temperature: 25°C, Sample : Naphthalene

Comparison of Backpressures

SunShell C18 column shows less than half the backpressure compared to sub-2 µm C18 columns.

Column dimension: 50 x 2.1 mm, Mobile phase: Acetonitrile/water=(70/30), Temperature: 25°C

Under a constant backpressure condition, SunShell C18 column shows two times higher performance than sub-2 µm columns.

Transfer from conventional 5 µm column to SunShell

By providing significantly increased column efficiency and resolution without the high back pressure, chromatographers can achieve UHPLC results on HPLC system.

HPLC system

100 mm of SunShell column provides the same number of theoretical plates (efficiency) as the 250 mm of conventional fully porous 5 µm column. Consequently, the analysis time can be reduced to 1/3 in the HPLC system.

Conventional C18, 5 µm 4.6 mm x 250 mm, SunShell C18, 2.6 mm 4.6 mm x 100 mm

UHPLC system

In the UHPLC system, the theoretical plates can be further increased by about 20 % when using a higher flow rate. The analysis time can be reduced to 1/6 compared with 250 mm of conventional fully porous 5 µm column.

SunShell C18, 2.6 mm 4.6 mm x 100 mm

Mobile phase	: CH3CN/20mM, Phosphoric acid = 45/55, Flow rate	: 1.0 mL/min, 1.8 mL/min with UHPLC system, Temperature	: 25 °C, Pressure	: 9.5 MPa for Conventional 5 µm C18, 13.4 MPa for SunShell 2.6 µm C18, Detection	: UV@230 nm, Sample	: 1 = Benzydamine 2 = Ketoprofen 3 = Naproxen 4 = Indomethacin 5 = Ibuprofen , HPLC	: Hitachi LaChrom ELITE (0.25 mm I.D. tubing), UHPLC	: Jasco X-LC

Comparison of Peak Tailing

Pyridine was used as a test of surface silanol activity. Only SunShell C18 column showed a sharp peak for pyridine.

Column: Brand K C18, 2.6 mm 150 x  4.6 mm, Brand A C18, 2.7 mm150 x 4.6 mm, SunShell C18, 2.6 mm 150 x 4.6 mm, Mobile phase:  CH3OH/H2O=30/70, Flow rate:1.0 mL/min, Temperature:40 °C, Detection:UV@250nm, Sample: 1 = Uracil, 2 = Pyridine, 3 = Phenol

Accelerated Low pH Stability Test

Under accelerated Low pH stability test, pH 1.0 at 80 °C, SunShell C18 column has kept 90% retention for 100 hours.

Test condition - Column size:  50 x 2.1 mm, Mobile phase:CH3CN/1.0% TFA, pH1=10/90, Flow rate: 0.4 mL/min, Temperature: 80 °C

Accelerated High pH Stability Test

Under accelerated pH 10 stability test, SunShell C18 column shows superior stability compared with other core shell C18 phases.

Test condition - Column Size: 50 x 2.1 mm, Mobile phase:CH3OH, 20mM Sodium borate, 10mM NaOH =30/21/49(pH10), Flow rate: 0.4 mL/min, Temperature: 50 °C

Loading Capacity of Amitriptyline

A highly basic compound, Amitriptyline was used for loading capacity test. SunShell showed up to 100 times higher loading capacity compared to other core shell columns.

Mobile phase: Acetonitrile/20mM phosphate buffer pH7.0=(60:40), Column dimension: 150 x 4.6 mm, Flow rate: 1.0 mL/min, Temp.: 407deg;C

Comparison with other core shell columns

Retention and back pressure
SunShell shows the highest retention and the lowest backpressure compared with other core shell columns.

Retention and back pressure

Chelating compound
Metal impurities in the core shell particle cause the peak tailing for 8-Quinolinol (Oxine).

Chelating compound

Acidic compound
Formic acid is used for the test of peak tailing. SunShell, Brand K and P show sharp peaks.

Acidic compound

SunShell C18 Application Data: Catechins in Oolong Tea

Mobile phase	: A) 0.1% Phosphoric acid
  	  B) CH3CN
Gradient program: Time    0 min.   7.5 min.   10 min.
                                   % B      2%         25%          25%
Flow rate	: 1.0 mL/min, 
Temperature	: 25 °C
Detection	: UV@250 nm
Sample	: Oolong tea

Gallocatechin, Epigallocatechin, Catechin, Caffeine, Epicatechin, Epigallocatechin gallate, Gallocatechin gallate, Epicatechin gallate, Catechin gallate

SunShell C18 Application Data: Dansylated estrogen hormones

SunShell C18  2.1 mm x 100 mm
Samples: 1. Dansylated estriol
                 2. Dansylated 17beta-estradiol
                 3. Dansylated 17alpha-estradiol        
                 4. Dansylated estrone
Mobile phase	: A) H2O with 0.1% formic acid.
		  B) CH3CN with 0.1% formic acid.
Gradient program: 0 - 0.5 min: 10% B
		  0.51 -3.0 min: 10 - 72% B
		  3.01 - 6.0 min: 72% B
		  6.01 - 7.0 min: 72 - 100% B
		  7.01 - 10.0 min: 100% B
Flow rate	: 0.45 mL/min.
Temperature	: 40 °C
Courtesy of Department of Chemistry & Biochemistry, The University of Texas at Arlington

SunShell RP Guard Filter (Cartridge Type)

Connecting Tube, Holder, Guard Filter

- C18 bonded porous glass filter (4 mm ID, 4 mm thickness)
- Low dead volume
- Compatible with the column I.D. from 2.1 mm to 4.6 mm

Ordering Information

SunShell C18
Product Cat.No. Size Quantity Price(US$)  
SunShell C18 CB6141 1.0 mm I.D. x 50 mm 1 pkg 580.00 Buy
SunShell C18 CB6161 1.0 mm I.D. x 100 mm 1 pkg 690.00 Buy
SunShell C18 CB6171 1.0 mm I.D. x 150 mm 1 pkg 740.00 Buy
SunShell C18 CB6931 2.1 mm I.D. x 30 mm 1 pkg 570.00 Buy
SunShell C18 CB6941 2.1 mm I.D. x 50 mm 1 pkg 580.00 Buy
SunShell C18 CB6951 2.1 mm I.D. x 75 mm 1 pkg 630.00 Buy
SunShell C18 CB6961 2.1 mm I.D. x 100 mm 1 pkg 690.00 Buy
SunShell C18 CB6971 2.1 mm I.D. x 150 mm 1 pkg 740.00 Buy
SunShell C18 CB6331 3.0 mm I.D. x 30 mm 1 pkg 570.00 Buy
SunShell C18 CB6341 3.0 mm I.D. x 50 mm 1 pkg 580.00 Buy
SunShell C18 CB6351 3.0 mm I.D. x 75 mm 1 pkg 630.00 Buy
SunShell C18 CB6361 3.0 mm I.D. x 100 mm 1 pkg 690.00 Buy
SunShell C18 CB6371 3.0 mm I.D. x 150 mm 1 pkg 740.00 Buy
SunShell C18 CB6381 3.0 mm I.D. x 250 mm 1 pkg 980.00 Buy
SunShell C18 CB6431 4.6 mm I.D. x 30 mm 1 pkg 570.00 Buy
SunShell C18 CB6441 4.6 mm I.D. x 50 mm 1 pkg 580.00 Buy
SunShell C18 CB6451 4.6 mm I.D. x 75 mm 1 pkg 630.00 Buy
SunShell C18 CB6461 4.6 mm I.D. x 100 mm 1 pkg 690.00 Buy
SunShell C18 CB6471 4.6 mm I.D. x 150 mm 1 pkg 740.00 Buy
SunShell C18 CB6481 4.6 mm I.D. x 250 mm 1 pkg 980.00 Buy
SunShell C8
Product Cat.No. Size Quantity Price(US$)  
SunShell C8 CC6931 2.1 mm I.D. x 30 mm 1 pkg 570.00 Buy
SunShell C8 CC6941 2.1 mm I.D. x 50 mm 1 pkg 580.00 Buy
SunShell C8 CC6951 2.1 mm I.D. x 75 mm 1 pkg 630.00 Buy
SunShell C8 CC6961 2.1 mm I.D. x 100 mm 1 pkg 690.00 Buy
SunShell C8 CC6971 2.1 mm I.D. x 150 mm 1 pkg 740.00 Buy
SunShell C8 CC6331 3.0 mm I.D. x 30 mm 1 pkg 570.00 Buy
SunShell C8 CC6341 3.0 mm I.D. x 50 mm 1 pkg 580.00 Buy
SunShell C8 CC6351 3.0 mm I.D. x 75 mm 1 pkg 630.00 Buy
SunShell C8 CC6361 3.0 mm I.D. x 100 mm 1 pkg 690.00 Buy
SunShell C8 CC6371 3.0 mm I.D. x 150 mm 1 pkg 740.00 Buy
SunShell C8 CC6431 4.6 mm I.D. x 30 mm 1 pkg 570.00 Buy
SunShell C8 CC6441 4.6 mm I.D. x 50 mm 1 pkg 580.00 Buy
SunShell C8 CC6451 4.6 mm I.D. x 75 mm 1 pkg 630.00 Buy
SunShell C8 CC6461 4.6 mm I.D. x 100 mm 1 pkg 690.00 Buy
SunShell C8 CC6471 4.6 mm I.D. x 150 mm 1 pkg 740.00 Buy
SunShell RP Guard Filter (Cartridge Type)
Product Cat.No. Size Quantity Price(US$)  
SunShell RP Guard Filter CBGAAC - 5 pieces 250.00 Buy
SunShell RP Guard Filter Holder CBGAAH - 1 piece 150.00 Buy
Connecting Tube PCO60K - 1 piece 70.00 Buy
SunShell RP Guard Filter Starter Kit (1 guard filter, 1 holder, 1 connecting tube) CBGAKN 1 set 260.00 Buy

The listed price above does NOT reflect the 30% discount. Please refer to your order confirmation e-mail to verify the discount.