Anti-GST (Mouse IgG2a-k), Monoclonal (GS019)
| Clone | : | GS019 |
| Isotype | : | IgG2a-κ (Mouse) |
| Product form | : | Liquid |
| Immunogen | : | Gulutathione-S-Transferase (GST) |
| Application | : | Western Blotting 1:1000-1:2000 Immunoprecipitation 1:400-1:1000 ELISA 1:2000-1:20000 |
Western Blotting
Sample:
1. Lysate from E. coli expressioning GST protein
2. Glutathione Sepharose flow-through fraction
3. Glutathione Sepharose elution fraction
| Gel | : | 12.5% SDS-PAGE |
| Blocking | : | Blocking One (Nacalai Tesque), 1hr at RT |
| Primary antibody | : | Anti-GST(Mouse IgG2a-κ)(1:2000 with Blocking One), 1hr at RT |
| Wash | : | TBS-T (5 min x 3) |
| Secondary antibody | : | Anti-Mouse Ig-HRP (1:1000 with TBS-T), 30 min at RT |
| Wash | : | TBS-T (5 min x 3) |
| Detection | : | Luminol Reagent (Santa Cruz) |
Data courtesy of Dr. Y. Shimizu, The Institute of Medical Science, The University of Tokyo
Downloads
Epitope Tag Antibodies (Brochure)Publications
Ordering Information
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| Anti-GST (Mouse IgG2a-k), Monoclonal (GS019) | 04435-26 | 2-8 °C | 200 µg | 298.00 | Buy |