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Anti-GST (Mouse IgG2a-k), Monoclonal (GS019)

Clone : GS019
Isotype : IgG2a-κ (Mouse)
Product form : Liquid
Immunogen : Gulutathione-S-Transferase (GST)
Application : Western Blotting 1:1000-1:2000
Immunoprecipitation 1:400-1:1000
ELISA 1:2000-1:20000

Western Blotting

Sample:
1. Lysate from E. coli expressioning GST protein
2. Glutathione Sepharose flow-through fraction
3. Glutathione Sepharose elution fraction

 
Gel : 12.5% SDS-PAGE
Blocking : Blocking One (Nacalai Tesque), 1hr at RT
Primary antibody : Anti-GST(Mouse IgG2a-κ)(1:2000 with Blocking One), 1hr at RT
Wash : TBS-T (5 min x 3)
Secondary antibody : Anti-Mouse Ig-HRP (1:1000 with TBS-T), 30 min at RT
Wash : TBS-T (5 min x 3)
Detection : Luminol Reagent (Santa Cruz)

Data courtesy of Dr. Y. Shimizu, The Institute of Medical Science, The University of Tokyo

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Anti-GST (Mouse IgG2a-k), Monoclonal (GS019) 04435-26 2-8 °C 200 µg 260.00 Buy