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Anti-HA (Mouse IgG1a-k), Monoclonal (HA124)

Clone : HA124
Isotype : IgG1a-κ (Mouse)
Product form : Liquid
Immunogen : Glutathione-S-Transferase (GST)-HA [YPYDVPDYA-COOH] fusion protein
Application : Western Blotting 1:1000-1:2000
ELISA 1:2000-1:20000

Western Blotting

Sample : 1. E.coli lysate
2. NH2-(GST)(T7)(HSV)(c-Myc)(VSV)(Glu-Glu)(V5)(E-tag)
    (DDDDK)(S-tag)(HA)(6xHis)-COOH, (abcam, #ab5395) 250 ng
3. NH2-(GST)(HA)-COOH (Pierce, #23613) 20 ng
4. NH2-(GST)(HA)-COOH (Pierce, #23613) 100 ng
5. M.W. marker
Primary antibody : Anti-HA (Mouse IgG1-κ), Monoclonal (HA124), 1:10000, 60 min
Secondary antibody : Anti-Mouse IgG (Rat), Monoclonal Cocktail, CC, POD Conjugated, 1:25000, 60 min
Detection : Chemi-Lumi One (Cat. No.: 05027-20), 1 min

Immunoprecipitation

Sample : 1. Clarified bacterial lysate
   + HA-tagged GST 1µg
   + Anti-HA (Mouse IgG1-κ), Monoclonal (HA124), 5 µg
   + Protein G Agarose (50% Suspention) 0.05 ml
2. E. coli lysate
   + NH2-(GST)(HA)-COOH (Pierce, #23613) 1 µg
Detection : Silver staining

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Anti-HA (Mouse IgG1-k), Monoclonal (HA124) 06340-54 2-8 °C 200 µg 260.00 Buy