for nucleic acid digestion to reduce viscosity of protein sample
TurboNuclease is a recombinant form of Serratia macescens extracellular endonuclease (encoded by the same gene of Bezonase) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both singleand double -stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
One unit of TurboNuclease converts 1 OD260 of salmon sperm DNA into acidsoluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 ug of salmon sperm DNA into oligonucleotides. TurboNuclease has a specific activity of >1.3x106 units/mg. This is equivalent to >3x106 Kunitz units/mg, over 100-fold specific activity of most highly purified bovine DNase I (~25,000 Kunitz units/mg).
50 ug of salmon sperm DNA was incubated with the indicated units of TurboNuclease and another brand of nuclease at 37°C for 30 minutes in a buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. DNA digestion was monitored by agarose gel. TurboNuclease shows no detectable protease activity.