Nacalai USA - Innovations for Life Sciences

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TurboNuclease

for nucleic acid digestion to reduce viscosity of protein sample

TurboNuclease is a recombinant form of Serratia macescens extracellular endonuclease (encoded by the same gene of Bezonase) produced in E. coli using a proprietary process. This nonspecific endonuclease hydrolyzes both singleand double -stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. TurboNuclease is a highly purified homodimer of 27 kDa subunits that has exceptional high specific activity and is free of protease activity. TurboNuclease is ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.

Features

  • Affordable ultra-pure Nuclease (Benzonase equivalent)
  • Free of protease activity

Activity and Specificity

One unit of TurboNuclease converts 1 OD260 of salmon sperm DNA into acidsoluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. This corresponds to complete digestion of 50 ug of salmon sperm DNA into oligonucleotides. TurboNuclease has a specific activity of >1.3x106 units/mg. This is equivalent to >3x106 Kunitz units/mg, over 100-fold specific activity of most highly purified bovine DNase I (~25,000 Kunitz units/mg).

Figure 1
50 ug of salmon sperm DNA was incubated with the indicated units of TurboNuclease and another brand of nuclease at 37°C for 30 minutes in a buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2. DNA digestion was monitored by agarose gel. TurboNuclease shows no detectable protease activity.

Formulation and Purity

TurboNuclease: 250 units/ul in Storage Buffer of 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% Glycerol. TurboNuclease is purified through a proprietary process that achieves purity of >99% as shown in Figure 2. Total endotoxin level is <0.1 EU/1,000 units of TurboNuclease as determined by the LAL Gel-Clot Assay.

Storage

Store TurboNuclease at -20°C. TurboNuclease is stable in the Storage Buffer at 37°C for at least three week without any loss of activity.

Application

TurboNuclease can be used to reduce viscosity of cell lysates and remove nucleic acid contamination from sample preparations. It reduces smearing when used with 10% SDS to make whole cell lysate for SDS-PAGE. It may reduce or prevent clumping of concentrated cells and frozen cells following thawing. TurboNuclease also replaces crude DNase I in many applications. To reduce viscosity of cell lysate, 10-500 units of TurboNuclease can be used for each gram of cell paste. The efficiency of viscosity reduction may vary with buffers, cell types, and cell lysis methods used. Due to its high specificity, the total amount TurboNuclease added is less than 0.1 ug/ml of lysate and will not complicate any down stream process.

Technical Information

TurboNuclease Procedure (PDF)

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
TurboNuclease (250 units/ul) NU0103M -20°C 50,000 units 210.00 Buy
TurboNuclease (250 units/ul) NU0103L -20°C 250,000 units 840.00 Buy