ES Quality Recombinant Human
Human VTN (Vitronectin) is a 478 amino acid protein (1-19 = signal domain) that belongs to a member of the pexin family. Vitronectin is an abundant glycoprotein found in serum and the extracellular matrix. It promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Vitronectin has been speculated to be involved in hemostasis and tumor malignancy. Recent publication from James Thomson's group indicated that coated recombinant human vitronectin protein alone benefits iPS cell generation when combined with E8 culture medium.
Human ES cells (H1) were cultivated in xeno-free medium (NutriStem™) on Vitronectin-398™ (Xeno-Free) coated 6-well plate for 10 generations, and staining With Oct4, TRA-81 and DAPI.
Recombinant human Vitronectin gene (20-398 aa Fragment) was constructed with codon optimization and expressed in non-fusion protein form in E.coli as inclusion bodies. The final product was refolded using our unique "temperature shift inclusion body refolding" technology and chromatographically purified. Coating this recombinant protein at 5-10 µg / well (6 well plate) in either NutirStem or E8 medium can be used for human iPS cell generation in vitro.
-80℃ for long term storage.
|Vitronectin-398™ (Xeno-free), Recombinant Human||NU0006||-20°C||500 µg||99.00||Buy|