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COSMOSIL 3C18-EB

Reversed Phase

  • Excellent for Basic compounds
  • Improved separation for acidic or metal coordination compounds
  • Simple mobile phase conditions

 

Analysis of Basic Compounds

Conventionally end-capped C18 columns still have many residual silanol groups on the silica surface of stationary phase that can form ionic bonds with basic compounds. The resulting peak tailing makes accurate quantification of analytes difficult, especially in trace analyses. COSMOSIL 3C18-EB with a better end-capping treatment offers improved peak shape and separation particularly for basic compounds.

Comparison 1 (Basic Compounds)

COSMOSIL 3C18-EB Competitor A
C18, 3 µm
Competitor B
C18, 3 µm
Competitor C
C18, 3 µm
Column size: 4.6 mm I.D. x 75 mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate
Buffer(pH7.0) = 60/40
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Amitriptyline 0.2mg/ml
2. Propylbenzene (I.S.) 2.0mg/ml

Inj. Vol: 2.0 µl

Comparison 2 (Basic Compounds)

COSMOSIL 3C18-EB Competitor A
C18, 3 µm
Competitor B
C18, 3 µm
Competitor C
C18, 3 µm
Column size: 4.6 mm I.D. x 75 mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate
Buffer(pH7.0) = 60/40
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 234 nm
Sample: 1. Dextromethorphan 0.5mg/ml
2. Benzene (I.S.) 7.5mg/ml

Inj. Vol: 1.5 µl

Analysis of Acidic or Metal Coordination Compounds

Acidic or metal coordination compounds can also form bonds with residual silanol groups and metal impurities causing peak tailing. COSMOSIL 3C18-EB with a better end-capping treatment and high purity silica gel offers improved peak shape and separation for acidic or metal coordination compounds.

Acidic Compounds

COSMOSIL 3C18-EB
Column size: 4.6 mm I.D. x 75 mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate
Buffer(pH2.5) = 30/70
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Salicylic Acid 1.0 mg/ml
2. Ethyl p-Hydroxybenzoate (I.S.) 0.05 mg/ml

Metal Coordination Compounds

COSMOSIL 3C18-EB
Column size: 4.6 mm I.D. x 75 mm
Mobile phase: Acetonitrile/ 20mmol/l
Phosphoric Acid = 10/90
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Oxine-copper 0.03 mg/ml
2. Caffeine (I.S.) 0.3 mg/ml

Inj. Vol: 2.0 µl

Synthesis Reproducibility

If you use insufficiently end-capped C18 columns for basic compound analyses, you may need to spend much time adjusting mobile phase conditions. You may need
  1. more than 3 or more organic solvents or buffers
  2. ion-pair reagents or additives
  3. buffers in different pH

In addition, the complexity of the mobile phase often is detrimental to reproducibility. COSMOSIL 3C18-EB with a new end-capping treatment allows good peak shape and separation using simple mobile phase conditions.

Analysis with Simple Condition

Complicated Condition Simple Condition
Methanol/ Acetonitrile/ 20mmol/l KH2PO4, 0.2%Triethylamine(pH3.0 with H3PO4) = 35/15/50(*) Acetonitrile/ 20mmol/l Phosphate Buffer(pH2.5) = 30/70
(*): USP 38(1) Amlodipine and Benazepril Hydrochloride Capsules, Performance Tests  
Column size: 4.6 mm I.D. x 100 mm
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 237 nm
Sample: 1. Amlodipine 0.0077mg/ml
2. Benazepril 0.0225mg/ml
Inj. Vol: 50 µl

Routine Analysis

3C18-EB has excellent synthesis reproducibility and column-to-column reproducibility, so it is applicable in routine analysis such as quality control of drugs.

Excellent Synthesis Reproducibility

COSMOSIL 3C18-EB
Column size: 4.6 mm I.D. x 150 mm
Mobile phase: Acetonitrile / H2O = 60/40
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Acetophenone 0.05 mg/ml
2. Methyl Benzoate 0.5 mg/ml
3. Benzene 2.0 mg/ml
4. Toluene 2.0 mg/ml
Inj. Vol: 1.5 µl
COSMOSIL 3C18-EB
Column size: 4.6 mm I.D. x 150 mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate
Buffer(pH7.0) = 60/40
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Amitriptyline 0.2 mg/ml
2. Propylbenzene (I.S.) 2.0 mg/ml
Inj. Vol: 2.0 µl

Analysis under Ammonium Acetate Buffer

COSMOSIL 3C18-EB produces sharp peaks using ammonium acetate buffer in LC-MS.

Excellent Synthesis Reproducibility

Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 60/40 Acetonitrile/ 10mmol/l CH3COONH4 = 60/40
Column size: 4.6 mm I.D. x 75 mm
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Amitriptyline 0.2mg/ml
2. Propylbenzene (I.S.) 2.0mg/ml

Inj. Vol: 1.0 µl

Analysis of standards in USP

Excellent Synthesis Reproducibility

COSMOSIL 3C18-EB
Column size: 4.6 mm I.D. x 100 mm
Mobile phase: A; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 10/90
B; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 80/20
B conc. 0→ 20%(0→ 10min), 20→ 100%(10→ 30min)
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 302 nm
Sample: 1. Omeprazole related compound A 0.001mg/ml
2. Omeprazole 0.001mg/ml
Inj. Vol: 20 µl
USP 37(3) Esomeprazole Magnesium Delayed-Release Capsules, Impurities

Ordering Information

Analytical
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL 3C18-EB Packed Column, 2.0 mm I.D. x 50 mm 09794-21 Waters type 1 pkg 360.00 Buy
COSMOSIL 3C18-EB Packed Column, 2.0 mm I.D. x 75 mm 09795-11 Waters type 1 pkg 390.00 Buy
COSMOSIL 3C18-EB Packed Column, 2.0 mm I.D. x 100 mm 09796-01 Waters type 1 pkg 410.00 Buy
COSMOSIL 3C18-EB Packed Column, 2.0 mm I.D. x 150 mm 09797-91 Waters type 1 pkg 450.00 Buy
COSMOSIL 3C18-EB Packed Column, 2.0 mm I.D. x 250 mm 09798-81 Waters type 1 pkg 590.00 Buy
COSMOSIL 3C18-EB Packed Column, 3.0 mm I.D. x 50 mm 09799-71 Waters type 1 pkg 360.00 Buy
COSMOSIL 3C18-EB Packed Column, 3.0 mm I.D. x 75 mm 09800-21 Waters type 1 pkg 390.00 Buy
COSMOSIL 3C18-EB Packed Column, 3.0 mm I.D. x 100 mm 09811-81 Waters type 1 pkg 410.00 Buy
COSMOSIL 3C18-EB Packed Column, 3.0 mm I.D. x 150 mm 09814-51 Waters type 1 pkg 450.00 Buy
COSMOSIL 3C18-EB Packed Column, 3.0 mm I.D. x 250 mm 09827-91 Waters type 1 pkg 590.00 Buy
COSMOSIL 3C18-EB Packed Column, 4.6 mm I.D. x 50 mm 09840-01 Waters type 1 pkg 360.00 Buy
COSMOSIL 3C18-EB Packed Column, 4.6 mm I.D. x 75 mm 09841-91 Waters type 1 pkg 390.00 Buy
COSMOSIL 3C18-EB Packed Column, 4.6 mm I.D. x 100 mm 09842-81 Waters type 1 pkg 410.00 Buy
COSMOSIL 3C18-EB Packed Column, 4.6 mm I.D. x 150 mm 09843-71 Waters type 1 pkg 450.00 Buy
COSMOSIL 3C18-EB Packed Column, 4.6 mm I.D. x 250 mm 09844-61 Waters type 1 pkg 590.00 Buy
Guard Column/Cartridge
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL 3C18-EB Guard Column, 4.6 mm I.D. x 10 mm 09839-41 Waters type 1 pkg 240.00 Buy
COSMOSIL 3C18-EB Guard Cartridge, 2.0 mm I.D. x 10 mm 11892-74 Waters type 2 pkg 170.00 Buy
COSMOSIL 3C18-EB Guard Cartridge, 4.6 mm I.D. x 10 mm 11890-94 Waters type 2 pkg 170.00 Buy
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 250.00 Buy
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) 38009-79 Waters type 1 pkg 250.00 Buy