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Prestained Protein Ladder, Broad Range

Protein Ladder

The Prestained Protein Ladder, Broad Range is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine builer). The Prestained Protein Ladder, Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

  • 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
  • 1.5~2.5 µl per well for general Western transferring.
  • Apply more for thicker (> 1.5 mm) or larger gel.
Tris-Glycine 4-20%, Bis-Tris 10% BOPS buffer, Bis-Tris 10% MES buffer

Contents
Approximately 0.1-0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Quality Control
Under suggested conditions, Prestained Protein Ladder, Broad Range resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.

Storage
Stable for up to 2 weeks at 25°C
Stable for up to 3 months at 4°C
For long term storage, store at -20°C

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Prestained Protein Ladder, Broad Range NU01002 -20°C 500 µl 100.00 Buy