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Total RNA Purification Plus Kit

For the purification of total RNA - including microRNA - with rapid gDNA removal

Norgen's Total RNA Purification Plus Kit provides a rapid method for the isolation and purification of total RNA in as little as 20 minutes. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), without the use of phenol or chloroform. Genomic DNA is removed from the sample using a Genomic DNA Removal column, and the RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. Norgen's kit is the only kit on the market that isolates true total RNA, as other kits must use phenol to recover all sizes of RNA.

Features

  • No phenol:chloroform extractions - Total RNA is isolated without the use of harmful chemicals such as phenol or chloroform
  • Rapid removal of genomic DNA - Contaminating gDNA is rapidly removed without the use of enzymes via a Genomic DNA Eliminator Column
  • Isolate total RNA from very small samples - Total RNA has been isolated and detected from as little as a single animal cell
  • Extremely efficient isolation of low abundance microRNA - Norgen's Total RNA Purification Plus Kit has been shown to be extremely efficient at recovering low abundance microRNA from plasma samples
  • Isolate a diversity of RNA species - All RNA species can be isolated, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
  • Fast and easy processing - Rapid spin - column format allows for the processing of 10 samples in 20 minutes
  • Isolate total RNA from a broad input source - Total RNA has been isolated from cultured animal cells, small tissue samples, LCM samples, bacteria, yeast, fungi, plants, viruses and various bodily fluid including blood, plasma, serum, saliva, nasal or throat swabs
  • No need for carrier RNA - Isolate all sizes of RNA without the use of carrier RNA
  • Multiple kit sizes available - This kit is available in both 50 prep and 100 prep sizes

Application 1 : Genomic DNA Removal from HeLa cells.

Norgen's Total RNA Isolation Plus Kit is unique in that it contains a Genomic DNA Removal Column, allowing for gDNA removal without the use of enzymes. HeLa cells were either passed through the gDNA Removal Column, or received no gDNA removal treatment. The Genomic DNA Removal Column successfully removed gDNA from both 1 million HeLa cells (Panel A) and 100,000 cells (Panel B). For both Panels A and B, the left image displays the RNA run on a 1.2% EtBr-agarose gel, to better visualize any gDNA contamination. The right image displays a 1.0% formaldehyde-agarose gel to allow for better denaturation of RNA in the sample. For higher inputs, as in image A, the Genomic DNA Removal Column also resulted in higher RNA yields than the non-treated samples.

Application 2 : Delta Ct Values Observed Between Different gDNA Removal Techniques.

Genomic DNA is well known to contaminate RNA samples, and subsequently RT-qPCR results. Therefore, to determine the amount of genomic DNA contamination in RNA samples, an RT-qPCR can be used to compare Ct values observed with and without reverse transcription of the RNA sample. This is known as the Delta (Δ) Ct. One million and 100,000 HeLa cells were either passed through the gDNA Removal Column or subjected to DNase-treatment using Norgen's RNase-Free DNase I (Cat. No.: 25710). The gDNA Removal column eliminated gDNA similar to a DNase treatment. For higher inputs (i.e. 1 Million HeLa cells), the gDNA Removal Column successfully removed more gDNA contamination from the RNA samples than the DNase-treatment. When inputs were lowered, the amount of genomic DNA contamination in the DNase-treated sample was similar to the sample passed through the gDNA Removal Column. Both methods work well for removing gDNA contamination in RNA samples.

Kit Specificaations

Maximum Binding Capacity 50 µg
Maximum Loading Volume Per Spin Column 650 µL
Size of RNA Purified All sizes, including < 200 nt
Time to Complete 10 Purifications 20 minutes
RNA Yield HeLa (1 x 106): 15 µg
E coli (1 x 109): 50 µg

Protocol & Product Information

Total RNA Purification Plus Kit - Protocol (PDF)
Total RNA Purification Plus Kit - Product Information (PDF)

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Total RNA Purification Plus Kit 48300 Room Temp. 50 preps 283.00 Buy
Total RNA Purification Plus Kit 48400 Room Temp. 100 preps 497.00 Buy