Rapid purification of total RNA -including microRNA - from small input amounts
Norgen's Total RNA Purification Micro Kit provides a rapid and sensitive method for the isolation and purification of total RNA from small input amounts of cultured animal cells, tissue samples, and microdissected samples including laser-capture microdissection (LCM). The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The special design of the micro spin-column allows a small elution volume of as little as 20 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell. Total RNA was extracted from a decreasing number of HeLa cells. Five µL of the eluted RNA was used as the template in a 20 µL RT-qPCR reaction to detect the human S14 transcript. The S14 was detected from as little as a single cell.
Norgen's Total RNA Purification Micro Kit allows sensitive but high quality RNA extraction from small inputs such as Laser-Captured Microdissection (LCM). Total RNA was isolated from 6 different LCM samples. RNA yield and quality (260/280 ratio) were assessed by NanoVue spectrophotometry. Norgen’s Total RNA Purification Kit recovered good amounts of RNA with excellent 260/280 ratio.
Norgen's Total RNA Purification Micro Kit allows sensitive RNA extraction from as little as a single cell and outperforms competitor's kits that claim similar sensitivity. Total RNA was extracted from a decreasing number of HeLa cells using Norgen's Total RNA Purification Micro Kit and a competitor's spin column purification kit. The extracted RNA was subjected to RT-qPCR to detect the human S15 and 5S rRNA transcript transcript in the isolated RNA. RNA isolated by Norgen's Total RNA Purification Micro Kit showed much higher relative expression of each RNA transcript tested at all cell input number down to single cell.
|Maximum Column Binding Capacity||35 µg|
|Maximum Column Loading Volume||650 µL|
|Minimum Elution Volume||20 µL|
|Size of RNA Purified||All sizes, including small RNA (<200 nt)|
|Maximum Amount of Starting Material||Animal Cells: 5 x 105 cells
Animal Tissues: 3 mg (for most tissues*)
Laser-Captured Microdissection (LCM): Up to 5 x 105 cells
|Time to Complete 10 Purifications||20 minutes|
|Average Yields||HeLa Cells (1 x 105 cells): 1.5 µg|
* for fibrous tissue, an additional Proteinase K treatment is required
|Total RNA Purification Micro Kit||35300||Room Temp.||50 preps||280.00||Buy|