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RNA/Protein Purification Plus Kit

For sequential isolation of total RNA and total proteins from the same sample

Norgen's RNA/Protein Purification Plus Kit provides a rapid method for the isolation and purification of total RNA (including small RNA and microRNAs) and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The total RNA and proteins are all column purified in less than 30 minutes. This kit is ideal for researchers who are interested in studying the transcriptome and proteome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen's RNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNAand proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Features

  • Complete column purification - The RNA and proteins are all column purified
  • Efficient gDNA removal - Non-enzymatic, rapid removal of DNA using a gDNA Removal Column
  • Reduce variability - RNA and proteins are isolated from a single sample with no splitting of the lysate, thus reducing inconsistent results and variability
  • Rapid procedure - Isolate total RNA and total proteins from a single sample in less than 30 minutes
  • Isolate a diversity of RNA species - All sizes of RNA are isolated, from large mRNA down to microRNA
  • Isolate high quality RNA and proteins - The purified RNA and proteins are of the highest quality and can be used in a number of downstream applications

Application1 : Recovery of True Total RNA including microRNA from Hamster Liver.

Panel A is a 1X MOPS 1% agarose gel showing 3 µL of 20 µL eluted RNA that was isolated from 2 different samples of 5 mg hamster liver using Norgen's RNA/DNA Purification Micro Kit. Norgen's RNA/DNA Purification Micro Kit isolated large RNA (represented by 28S and 18S rRNA) with high integrity. Moreover, it provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen's RNA/DNA Purification Micro Kit showed the added benefit of recovering small RNA while isolating very high quality RNA. The effectiveness in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for beta-Actin (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen's RNA/DNA Purification Micro Kit showed detection of both small RNA (Panel C) and the large RNA (Panel D).

Application2 : High Quality Total Proteins Eluted in Mass Spec-Compatible Buffer.

Norgen's RNA/Protein Purification Plus Kit provides a column purification step for effective concentration and clean-up of the isolated proteins. The proteins are eluted into a buffer which is compatible with many downstream applications including mass spectrometry as well as standard protein quantification methods (including Bradford assays). In contrast, most competing multiple analyte isolation products require protein precipitation and the precipitated proteins are required to be resuspended in buffer with high-detergent content (such as SDS-PAGE loading dye) for full recovery. In this figure, the protein fraction isolated from hamster liver using Norgen's RNA/Protein Purification Plus Kit was resolved direclty on a 12% SDS-PAGE protein gel. Norgen's RNA/Protein Purification Plus Kit purified the proteins by column and the eluted proteins are already in buffer compatible with downstream applications.

Kit Specificaations

Column Binding Capacity 50 µg (RNA)
200 µg (Protein)
Maximum Column Loading Volume 650 µL
Size of RNA Purified All sizes
Time to Complete 10 Purifications 30 minutes
Average Yields:
HEK 293 Cells (1 x 106 cells)
HEK 293 Cells (1 x 106 cells)
Liver (15 mg)
Liver (15 mg)
10 - 15 µg RNA
70 - 100 µg protein
30 - 35 µg RNA
100 - 150 µg protein

Protocol

RNA/Protein Purification Plus Kit (PDF)

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
RNA/Protein Purification Plus Kit 48100 Room Temp. 20 preps 204.00 Buy
RNA/Protein Purification Plus Kit 48200 Room Temp. 50 preps 476.00 Buy