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Phosphatase Inhibitor Cocktail (EDTA free) (100X)

EDTA free

Phosphatase Inhibitor Cocktail is a mixture of several inhibitors to protect valuable proteins from dephosphorylation. The product preserves phosphorylated proteins existing in small quantity in cells and tissues. This product does not interfere with metalloproteins since it is EDTA free.

  • Contains 6 kinds of phosphatase inhibitors for different targets.
  • 100 times concentrated stock solution. Dilute with any buffer of your choice.
  • Stable for two years in refrigerator (2-8 °C).
  • Ready to use.

Comparison data

The product inhibis Ser/Thr phosphatase, Tyrosine Phosphatase, Acid Phosphatase and Alkaline phosphatase.
The product preserves a small quantity of phosphorylated protein.

Composition

The product (100 times concentrated stock solution) contains:

Inhibitors Targeted phosphatase
Imidazole Alkaline Phosphatase
Sodium Orthovanadate (V) Protein Tyrosine Phosphatase
Alkaline Phosphatase
b-Glycerophosphoric Acid Disodium Salt Ser/Ther Phosphatase
Sodium (+)-Tartrate Dihydrate Acid Phosphatase
Sodium Fluoride Acid Phosphatase
Protein Phosphatase-2A
Protein Phosphatase-2B
Protein Phosphatase-2C
Disodium Molybdate (VI) Dihydrate Acid Phosphatase
Phosphoprotein phosphatase

Protocol

Dilute 100 fold with any buffer of your choice.

Reference

  1. Yang, JH. et al. The Journal of Biological Chemistry, Oct 2010.
  2. Selamat, W. et al. Neuroscience Letters 450(2), 163-166 (2009)
  3. Saito, T. et al. Biochemical and Biophysical Research Communications 357(2), 371-376 (2007)
  4. Murakami, Y. et al. J. Biochem., 141, 401-410 (2007)
  5. Takenaga, M. et al. J. Cell Sci., 120, 2078-2090 (2007)

Note

  • If phosphatase concentration is high, it can be diluted by 20-50 fold.
  • The product may cause inhibition of 2D electrophoresis. Remove inhibitors by dialysis or desalt before use.
  • The product does not contain protease inhibitors. For homogenizing cells and tissues, if necessary, add protease inhibitor cocktail, such as Protease Inhibitor Cocktail (EDTA free) (Cat. No.: 03969).
  • The product includes Imidazole for inhibitor. In case of Metal Affinity Purification, it is recommended a pretest with reducing Imidazole concentration in the buffer by 5 mM.
  • The product colors gradually.
  • Direct light may interfere with an inhibition effect; shield the product from the light after using.
  • The components may be precipitated during storage. Before use, melt it by vortex at 30-40°C shielding light.

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Phosphatase Inhibitor Cocktail (EDTA free) (100X) 07575-51 2-8 °C 1 ml 129.00 Buy