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FG beads - Protein A / Protein G beads

IgG purification / Immunoprecipitation (IP) / Chromatin Immunoprecipitation (ChIP) / Protein separation

     Product information

  Protein A Protein G
Storage temperature 4℃ (2-8℃), Don't freeze
Storage buffer 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol 10mM HEPES(pH7.9), 50mM KCl, 1mM EDTA, 10%glycerol
Magnetization superparamagnetism(<10 emu/g)
Size of beads 190 nm±20 nm
Concentration 20 mg/ml
Functional groups Protein A Protein G
Capacity >22 ug Human IgG/mg of beads >11 ug Human IgG/mg of beads

 

Features

  • High recovery
    IgG binding capacity - more than twice the amount of a competitor.
  • High purity
    Extremely low non-specific adsorption.
  • Quick processing
    30 minutes for IgG binding.

Applications

  • IgG purification
  • Immunoprecipitation (IP)
  • Chromatin Immunoprecipitation (ChIP)
  • Protein separation

IgG Purification

We compared the binding capacity of FG beads to capture IgG in HeLa cell extracts with the beads of a competitor. FG beads captured larger amounts of IgG than the competitor's beads.

  1. Add 10 µg of IgG into 200 µg of HeLa cell extracts (200 µl).
  2. Add 0.3 mg of each beads to the HeLa cell extracts.
  3. React for 10 min at 4°C and separate beads from the HeLa cell extract.
  4. Elute bound IgG by adding Glycine-HCl.

Immunoprecipitation

We checked the performance of FG beads (Protein A and Protein G) in an immunoprecipitation experiment. By using FG beads, antigen HSA was immunoprecipitated with high recovery and extremely low non-specific adsorption.

  1. Immobilize anti-Human Serum Albumin antibody on FG beads.
  2. Add 400 ng of HSA into 200 µg of HeLa cell extracts (200 µl).
  3. Add 0.1 mg of each beads to HeLa cell extracts.
  4. React for 120 min at 4°C and separate beads from the HeLa cell extract.
  5. Elute bound IgG and HSA by adding Glycine-HCl.

Protocol

Chemical pulldown

E001 Screening by using ligand immobilized beads 259KB
E003 Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads 111KB
E004 Immobilization of ligands (carboxylic compounds) on OH beads 78KB
E005 Immobilization of ligands (carboxylic compounds) on NH2 beads 97KB
E008 Immobilization of ligands (compounds with NH2 groups) on COOH beads 139KB
E012 Competitive inhibition 261KB
E013 Drug elution 260KB
E014 Immobilization of ligands (compounds with NH2 groups) on NHS beads 75KB
E015 Immobilization of MTX aminated derivatives on NHS beads 66KB
E108 Immobilization of Ligand on Streptavidin beads 102KB
E109 Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction 65KB
E201 Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) 1,329KB

Immunoprecipitation, Protein-protein interaction

E011 Immunoprecipitation 77KB
E101 Immobilization of proteins on COOH beads 83KB
E102 Immobilization of His-Tag proteins on Ts beads 89KB
E105 Immobilization of Antibodies or Proteins on NHS beads 23KB
E106 Immobilization of Antibodies or Proteins on Epoxy Beads 65KB
E107 Direct Quantification of Immobilized Proteins (Antibodies) 107KB
E108 Immobilization of Ligand on Streptavidin beads 102KB
E110 Immobilization of antibodies on Protein A beads and Protein G beads 103KB

Purification of DNA and RNA binding substances

E001 Screening by using ligand immobilized beads 259KB
E301 Immobilization of double strand DNA on Plain beads 143KB

Ordering Information

FG beads
Product Cat.No. Storage Quantity Price(US$)  
Protein A beads ** 50% OFF PROMOTION, List Price: $150 ** TAS8848 N1172 4°C 5 mg 75.00 Buy
Protein G beads ** 50% OFF PROMOTION, List Price: $150 ** TAS8848 N1173 4°C 5 mg 75.00 Buy
Magnetic Stand
Product Cat.No. Storage Quantity Price(US$)  
Magnetic Stand (for 1.5 ml tube) ** 30% OFF PROMOTION, List Price: $800 ** TA4899N12 Room Temp. 1 ea 560.00 Buy
Magnetic Stand (for 15 ml tube) TA4899N20 Room Temp. 1 ea 600.00 Buy
Magnetic Stand (for 50 ml tube) TA4899N30 Room Temp. 1 ea 700.00 Buy