Nacalai USA - Innovations for Life Sciences

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EZSPHERE™

Cell Culture Ware for Spheroid Formation

Three dimensional (3D) cell culture systems have gained in popularity as invaluable tools in broad applications of cell biology. 3D multi-cellular cell aggregates (Spheroid) can be formed by using a low attachment culture surface. However, variability in forming spheroids has been a persistent problem. EZSPHERE is specifically designed to form a large number of uniformly sized Spheroids and Embryoid Bodies (EBs).

Features

  • The surface of EZSPHERE is coated with very low binding 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer.
  • EZSPHERE has a lot of evenly designed micro wells on the surface. Inoculated cells drop into the micro wells and form uniformly sized Spheroids.
  • Spheroids can be formed efficiently in the round shape wells.

Publication

"Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells"

http://www.nature.com/articles/srep31063

Unique micro-fabricated plastic vessel

Useful for mass-production of EBs with uniform size

EB formation, proliferation and differentiation protocols

High efficient generation of EBs with uniform size

EB-size control with micro-well sizes or inoculating cell demsities

Proliferation of iPSCs with maintaining pluripotency

Induction of dopaminergic neurons from the EBs

Induction of cardiomyocyte from the EBs

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ Variety Pack (35 mm dish), 6ea / pack 4000-9VP Room Temp. 1 150.00 Buy
EZSPHERE™ Dish 35 mm Well Size (µm) ... Diameter: 400-500, Death: 100-200 No. of Well ... 2,300/dish 4000-900SP Room Temp. 10 450.00 Buy
EZSPHERE™ Dish 60 mm Well Size (µm) ... Diameter: 400-500, Death: 100-200 No. of Well ... 5,300/dish 4010-900SP Room Temp. 10 480.00 Buy
EZSPHERE™ Dish 100 mm Well Size (µm) ... Diameter: 400-500, Death: 100-200 No. of Well ... 14,000/dish 4020-900SP Room Temp. 10 500.00 Buy
EZSPHERE™ 6-well Plate Well Size (µm) ... Diameter: 400-500, Death: 100-200 No. of Well ... 2,400/well 4810-900SP Room Temp. 5 275.00 Buy
EZSPHERE™ 96-well Plate Well Size (µm) ... Diameter: 400-500, Death: 100-200 No. of Well ... 80/well 4860-900SP Room Temp. 5 300.00 Buy
EZSPHERE™ Dish 35 mm Type 902 Well Size (mm) ... Diameter: 500, Death: 200 No. of Well ... 2,300/dish 4000-902SP Room Temp. 10 450.00 Buy
EZSPHERE™ Dish 35 mm Type 903 Well Size (mm) ... Diameter: 800, Death: 300 No. of Well ... 1,000/dish 4000-903SP Room Temp. 10 450.00 Buy
EZSPHERE™ Dish 35 mm Type 904 Well Size (mm) ... Diameter: 800, Death: 400 No. of Well ... 600/dish 4000-904SP Room Temp. 10 450.00 Buy