Nacalai USA - Innovations for Life Sciences

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EZSPHERE™

Cell Culture Ware for Spheroid Formation


Three dimensional (3D) cell culture systems have gained in popularity as invaluable tools in broad applications of cell biology. 3D multi-cellular cell aggregates (Spheroid) can be formed by using a low attachment culture surface. However, variability in forming spheroids has been a persistent problem. EZSPHERE is specifically designed to form a large number of uniformly sized Spheroids and Embryoid Bodies (EBs).

Features

  • The surface of EZSPHERE is coated with very low binding 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer.
  • EZSPHERE has a lot of evenly designed micro wells on the surface -- 6 different well size available. 
    Micro well type

     Diameter (µm) 

        Depth (µm)    

     

    #901 200 100
    #900 500 100
    #902 500 200
    #903 800 400
    #904 800 300
    #905 1,400 600

Publication

"Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells"

http://www.nature.com/articles/srep31063

Unique micro-fabricated plastic vessel

Useful for mass-production of EBs with uniform size

EB formation, proliferation and differentiation protocols

High efficient generation of EBs with uniform size

EB-size control with micro-well sizes or inoculating cell densities

Proliferation of iPSCs with maintaining pluripotency

Induction of dopaminergic neurons from the EBs

Induction of cardiomyocyte from the EBs

Referred protocol

iPS spheroid formation in EZSPHERETM devices: http://www.atgc.co.jp/div/rika/hbin/ez2012.html

Ordering Information

#900
Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ 35 mm Dish, 10 dishes, Diameter: 500µm, Depth: 100µm, No. of Well: 2,310/dish 4000-900SP Room Temp. 10 375.00 Buy
EZSPHERE™ 60 mm Dish, 10 dishes, Diameter: 500µm, Depth: 100µm, No. of Well: 5,300/dish 4010-900SP Room Temp. 10 400.00 Buy
EZSPHERE™ 10 mm Dish, 10 dishes, Diameter: 500µm, Depth: 100µm, No. of Well: 14,000/dish 4020-900SP Room Temp. 10 425.00 Buy
EZSPHERE™ 6-well Plate, 5 plates, Diameter: 500µm, Depth: 100µm, No. of Well: 2,400/well 4810-900SP Room Temp. 5 250.00 Buy
EZSPHERE™ 96-well Plate, 5 plates, Diameter: 500µm, Depth: 100µm, No. of Well: 80/well 4860-900SP Room Temp. 5 250.00 Buy
#901
Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ 35 mm Dish, 10 dishes, Diameter: 200µm, Depth: 100µm 4000-901SP Room Temp. 10 N/A Inquire
#902
Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ 35 mm Dish, 10 dishes, Diameter: 500µm, Depth: 200µm, No. of Well: 2,310/dish 4000-902SP Room Temp. 10 380.00 Buy
#903
Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ 35 mm Dish, 10 dishes, Diameter: 800µm, Depth: 400µm, No. of Well: 1,030/dish 4000-903SP Room Temp. 10 380.00 Buy
EZSPHERE™ 12-well Plate, 10 plates, Diameter: 800µm, Depth: 400µm TCI-4815-903SP-10P Room Temp. 10 805.00 Buy
EZSPHERE™ 12-well Plate, 10 plates, Diameter: 800µm, Depth: 400µm TCI-4815-903SP-50P Room Temp. 50 3,000.00 Buy
EZSPHERE™ 96-well Plate, 10 plates, Diameter: 800µm, Depth: 400µm TCI-4860-903SP-10P Room Temp. 10 805.00 Buy
#904
Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ 35 mm Dish, 10 dishes, Diameter: 800µm, Depth: 300µm, No. of Well: 580/dish 4000-904SP Room Temp. 10 380.00 Buy
#905
Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ 35 mm Dish, 10 dishes, Diameter: 1,400µm, Depth: 600µm, No. of Well: 220/dish 4000-905SP Room Temp. 10 380.00 Buy
Variety Pack
Product Cat.No. Storage Quantity Price(US$)  
EZSPHERE™ Variety Pack (35 mm dish), 6ea / pack 4000-9VP Room Temp. 1 200.00 Buy