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Recombinant Human Transferrin, Animal-free

Cell Culture Supplements

Recombinant Human Transferrin, Animal-free has been produced from a plant based (rice) expression system. Human transferrin is responsible for iron transport in animals. In cells, intracellular iron is required to maintain cell growth. Transferrin is the preferred method for iron delivery into cells where it enters through the transferrin receptor. Each 80 kDa transferrin can transport two iron molecules. In cell culture applications, iron delivery by transferrin has advantages compared to free iron or iron-chelators. Free iron promotes free radical formation, which can damage cells. Iron-chelators tend to disrupt downstream processes and tend to have lower iron bioavailability, require excessive iron loading in cell culture media. Recombinant Human Transferrin, Animal-free is designed for use in cell culture media and life science research.

Optimal concentration

The optimum concentration of Human Transferrin for cell culture varies with the cell line. In CHO, effective concentrations range from 10-50 mg/L. The reported effective concentrations for hybridoma and Vero cell lines range from 5-20 mg/L. Effective concentrations for primary cells vary between 5-100 mg/L. Cell lines require little or no adaptation to Human Transfferin.

Preparation

Stock solutions can be prepared by dissolving gently into PBS for several minutes. Typical stock concentrations are 5-20 mg/ml in PBS, although others can be used. Avoid the formation of bubbles when dissolving. Filter through 0.2 µm filter to sterilize.

Specifications

  • Purity (SDS-PAGE): 95% or more
  • Moisture Content: 10% or less
  • Iron (Fe): 0.1% or less
  • Endotoxin: 1 EU/mg or less

Storage

Human Transferrin is stable in solution for several weeks at 4°C and can be stored frozen at -20°C for longer term storage. Avoid repeated freeze-thaw cycles of Human Transferrin solution.

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Recombinant Human Transferrin, Animal-free NU12879-1G -20°C 1 g 400.00 Buy