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COSMOSIL 5PY (2-Ethylpyridine)

COSMOSIL SFC COLUMN / 2-Ethylpyridine group

Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations.

 

COSMOSIL 5PY (2-Ethylpyridine)
Silicagel high purity porous spherical silica
Average particle size 3 and 5µm
Average pore size approx. 120Å
Specific surface area approx. 300m2/g

Application: Hydrophilic organics

Phase: 2-Ethylpyridine
Column size: 4.6 mmI.D. x 150 mm
Flow rate: 5.6 mL/min
Pressure: 140 bar
Temperature: ambient
Mobile phase: Methanol Gradient 5-50% @18%/minute; hold @ 50% 0.1 min; return to 5% @99%/min

Comparison of Retention Behavior

The following three stationary phases were evaluated for their retention of hydrophilic, hydrophobic, acidic and basic compounds. COSMOSIL HP and PY elute hydrophobic compounds first and retain hydrophilic compounds longer, whereas PBr elutes in the reverse order, exhibiting high retention for hydrophobic compounds. HP had the longest retention for basic compounds.

Column size: 4.6 mmI.D. x 150 mm
Mobile phase:   A: CO2
B: 0.1% CH3COONH4 - Methanol
B conc. 0→50% (0→14min), 50% (14-17min)
Flow rate: 0.8 ml/min
BPR 10 MPa
Temperature: 40°C
Detection: ESI-MS/MS, MRM
Sample 

(No.1)  Uridine
(No.2)  3’ ,5’ -Dibenzyloxyacetophenone
(No.3)  4-Ethylbenzenesulfonic Acid
(No.4)  Benzyltrimethylammonium

Sample conc. 8 µmol/l
Inj. Vol 2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Ordering Information

Particle Size: 3µm
Product Cat.No. Quantity Price(US$)  
COSMOSIL 3PY (2-Ethylpyridine) Packed Column, 3µm, 2.0 mm I.D. x 150 mm 13831-91 1 pkg N/A Inquire
COSMOSIL 3PY (2-Ethylpyridine) Packed Column, 3µm, 4.6 mm I.D. x 250 mm 13832-81 1 pkg N/A Inquire
Particle Size: 5µm
Product Cat.No. Quantity Price(US$)  
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 2.0 mm I.D. x 150 mm 13818-81(NU00690) 1 pkg 680.00 Buy
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 4.6 mm I.D. x 100 mm 13974-31(NU00700) 1 pkg 650.00 Buy
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 4.6 mm I.D. x 150 mm 13975-21(NU00701) 1 pkg 680.00 Buy
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 4.6 mm I.D. x 250 mm 13827-61 1 pkg N/A Inquire
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 10.0 mm I.D. x 20 mm 13830-01 1 pkg N/A Inquire
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 10.0 mm I.D. x 150 mm 13976-11(NU00702) 1 pkg N/A Inquire
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 10.0 mm I.D. x 250 mm 13828-51 1 pkg N/A Inquire
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 20.0 mm I.D. x 150 mm 13977-01(NU00703) 1 pkg N/A Inquire
COSMOSIL 5PY (2-Ethylpyridine) Packed Column, 5µm, 20.0 mm I.D. x 250 mm 13829-41 1 pkg N/A Inquire