The structural similarities between polar lipids, such as cholesterol and related analogs, have posed chromatographic and spectrometric challenges to analysts interested in quantifying these potential biomarkers. COSMOSIL Quinoline has been developed to improve the separation of these structural isomers utilizing the π-π interactions and structural rigidity of the naphthylethyl phase and the hydrogen bonding of the pyridine phase.
COSMOSIL Quinoline
Silicagel
high purity porous spherical silica
Average particle size
2.5 and 5µm
Average pore size
approx. 120Å
Specific surface area
approx. 300m2/g
Application 1: Steroids
Phase:
Quinoline
Column size:
4.6 mmI.D. x 150 mm
Mobile phase:
Carbon Dioxide (A) : Methanol (B)
Gradient:
2% - 7% B in 4 minutes
Flow rate:
4.5 ml/min
Pressure:
160 bar
Temperature:
30°C
Detection:
APCI(+)
Application 2: Caffeine Analogs
Phase:
Quinoline
Column size:
4.6 mmI.D. x 150 mm
Mobile phase:
Carbon Dioxide : Methanol (95:5)
Flow rate:
4.5 ml/min
Pressure:
160 bar
Temperature:
30°C
Detection:
UV260nm
Ordering Information
Particle Size: 2.5µm
Product
Cat.No.
Quantity
Price(US$)
COSMOSIL Quinoline Packed Column, 2.5µm, 3.0 mm I.D. x 50 mm