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COSMOSIL 5HP (3-Hydroxyphenyl)

COSMOSIL SFC COLUMNS / 3-Hydroxyphenyl group

Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations.

COSMOSIL 5HP (3-Hydroxyphenyl)
Silicagel high purity porous spherical silica
Average particle size 2.5, 3 and 5µm
Average pore size approx. 120Å
Specific surface area approx. 300m2/g

Application 1: Non-steroidal anti-inflammatory drugs

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Carbon Dioxide : Methanol
Gradient: 95:5 to 65:35 over 6 minutes
Flow rate: 5.0 ml/min
Temperature: 30°C
Detection: UV230nm
Sample: 1: Ibuprofen
2: Fenoprofen
3: Flurbiprofen
4: Ketoprofen
5: Indoprofen

 

Application 2: Beta Blockers

Peak elution order reversal under identical Conditions

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Carbon Dioxide : Methanol w/20mM ammonium formate
Gradient: 95:5 to 50:50 over 4 minutes
Flow rate: 5.0 ml/min
Temperature: 30°C
Detection: APCI(+)
Sample: 1. Pindolol
2. Atenolol

Comparison of Retention Behavior

The following three stationary phases were evaluated for their retention of hydrophilic, hydrophobic, acidic and basic compounds. COSMOSIL HP and PY elute hydrophobic compounds first and retain hydrophilic compounds longer, whereas PBr elutes in the reverse order, exhibiting high retention for hydrophobic compounds. HP had the longest retention for basic compounds.

Column size: 4.6 mmI.D. x 150 mm
Mobile phase:   A: CO2
B: 0.1% CH3COONH4 - Methanol
B conc. 0→50% (0→14min), 50% (14-17min)
Flow rate: 0.8 ml/min
BPR 10 MPa
Temperature: 40°C
Detection: ESI-MS/MS, MRM
Sample 

(No.1)  Uridine
(No.2)  3’ ,5’ -Dibenzyloxyacetophenone
(No.3)  4-Ethylbenzenesulfonic Acid
(No.4)  Benzyltrimethylammonium

Sample conc. 8 µmol/l
Inj. Vol 2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Ordering Information

Particle Size: 2.5µm
Product Cat.No. Quantity Price(US$)  
COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column, 2.5µm, 3.0 mm I.D. x 50 mm NU05002 1 pkg 790.00 Buy
COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column, 2.5µm, 3.0 mm I.D. x 100 mm NU05005 1 pkg 790.00 Buy
COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column, 2.5µm, 3.0 mm I.D. x 150 mm NU05006 1 pkg 790.00 Buy
Particle Size: 3µm
Product Cat.No. Quantity Price(US$)  
COSMOSIL 3HP (3-Hydroxyphenyl) Packed Column, 3µm, 2.0 mm I.D. x 150 mm 13792-11 1 pkg N/A Inquire
COSMOSIL 3HP (3-Hydroxyphenyl) Packed Column, 3µm, 4.6 mm I.D. x 250 mm 13793-01 1 pkg N/A Inquire
Particle Size: 5µm
Product Cat.No. Quantity Price(US$)  
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 2.0 mm I.D. x 150 mm 13787-91(NU00490) 1 pkg 680.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 4.6 mm I.D. x 100 mm 13971-61(NU00500) 1 pkg 650.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 4.6 mm I.D. x 150 mm 13972-51(NU00501) 1 pkg 680.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 4.6 mm I.D. x 250 mm 13788-81 1 pkg N/A Inquire
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 10.0 mm I.D. x 20 mm 13791-21 1 pkg N/A Inquire
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 10.0 mm I.D. x 150 mm 13973-41(NU00502) 1 pkg N/A Inquire
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 10.0 mm I.D. x 250 mm 13789-71 1 pkg N/A Inquire
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 20.0 mm I.D. x 150 mm 13855-91(NU00503) 1 pkg N/A Inquire
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column, 5µm, 20.0 mm I.D. x 250 mm 13790-31 1 pkg N/A Inquire