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COSMOGEL IEX Series

IEX

Ion Exchange Chromatography (IEX) has been widely used for analysis and purification of bio-molecules such as proteins, peptides and polynucleotides.
COSMOGEL IEX Series are available in 3 different ion exchange modes, Anion-exchange type, Cation-exchange type and Amphoteric ion-exchange type.

Exchange ModeAnion-exchange TypeCation-exchange TypeAmphoteric ion-exchange Type
Packing Material Type Q Type Q-N Type S Type S-N Type M Type M-N
Gel / Particle Size Hydrophilic Polymer / 5µm
Pore Size 1000 Å Non-porous 1000 Å Non-porous 1000 Å Non-porous
Functional Group -CH3N+(CH3)3 -(CH2)3SO3- -CH3N+(CH3)3 + -(CH2)3SO3-
Binding Capacity 110-150 mg 12-20 mg 70-100 mg 10-18 mg BSA:
55-75 mg/ml
IgG:
35-50 mg/ml
BSA:
6-10 mg/ml
IgG:
5-9 mg/ml
BSA/ml-resin human IgG/ml-resin
Target Sample Acidic Proteins / DNA Basic Proteins All Proteins
Application Purification Ultra-fast
Analysis
Precise
Analysis
Purification Ultra-fast
Analysis
Precise
Analysis
Purification Precise
Analysis
Column Size 4.6 x 50 4.6 x 30 4.6 x 100 4.6 x 50 4.6 x 30 4.6 x 100 4.6 x 50 4.6 x 100

 

Characteristic of Amphoteric ion-exchange

Amphoteric ion-exchange type (Type M) enables separation of both acidic and basic proteins in one sample at the same time.

COSMOSIL Application Data

Column: COSMOGEL IEX   Sample: 1; Ribonuclease A
2; Cytochrome C
3; Trypsin Inhibitor
4; β-Galactosidase
(1.5µg)
(1.5µg)
(6.0µg)
(6.0µg)
Column size: 4.6mmI.D.-50mm  
Mobile phase: A: 20mmol/l Phosphate buffer(pH6.8)
B: 20mmol/l Phosphate buffer(pH6.8)
   + 0.5mol/l NaCl
B conc. 0→100% 30min Linear gradient
 
Flow rate: 0.5 ml/min        
Temperature: 30°C        
Detection: UV220nm        

Amphoteric ion-exchange type (Type M) can be used as Anion-exchange type or Cation-exchange type.

COSMOSIL Application Data

Column: COSMOGEL IEX   Sample: 1. Carbonic Anhydrase
2. BSA
(0.8µg)
(6.3µg)
Column size: 4.6mmI.D.-50mm  
Mobile phase: A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3)
   + 0.5mol/l NaCl
B conc. 0→100% 30min Linear gradient
 
Flow rate: 0.5 ml/min        
Temperature: 30°C        
Detection: UV220nm        

Large sample Purification

Type Q, Type S and Type M with porous packing materials have higher binding capacity of proteins than the respective non-porous type, which means that peak shape does not spread even with injection of a large volume of sample. Therefore they are highly suitable for purification of large sample.

Column: COSMOGEL IEX Type S   Sample: 1. Ribonuclease A
2. Cytochrome C
3. Lysozyme
Column size: 4.6mmI.D.-50mm  
Mobile phase: A: 20mmol/l Phosphate buffer(pH6.8)
B: 20mmol/l Phosphate buffer(pH6.8)
   + 0.5mol/l NaCl
B conc. 0→100% 30min Linear gradient
 
Flow rate: 0.5 ml/min  
Temperature: 30°C  
Detection: UV280nm  

Precise Analysis

Type Q-N, Type S-N, Type M-N with non-porous packing materials reduce spreading of samples in packing materials, result in high resolution separation for precise analysis such as quality control of antibody drugs. The longer column length also contributes to the sharper peaks.

Column: COSMOGEL IEX   Sample: 1. Ribonuclease A
2. Cytochrome C
3. Lysozyme
(1.65µg)
(1.65µg)
(1.65µg)
Mobile phase: A: 20mmol/l Phosphate buffer(pH6.8)
B: 20mmol/l Phosphate buffer(pH6.8)
   + 0.5mol/l NaCl
B conc. 0→100% 30min Linear gradient
 
Flow rate: 0.5 ml/min  
Temperature: 30°C  
Detection: UV220nm  

Ultra-fast Analysis

The resolution with non-porous packing materials is not much affected by high flow rate and thus the materials are suitable for fast analysis. The shorter column length contributes to the fast analysis.

Column: COSMOGEL IEX   Sample: 1. Ribonuclease A
2. Cytochrome C
3. Lysozyme
(1.65µg)
(1.65µg)
(1.65µg)
Mobile phase: A: 20mmol/l Phosphate buffer(pH6.8)
B: 20mmol/l Phosphate buffer(pH6.8)
   + 0.5mol/l NaCl
B conc. 0→100% Linear gradient
Type S: 30min Type S-N: 10min
 
Flow rate: Type S: 0.5 ml/min,
Type S-N: 1.0 ml/min
 
Temperature: 30°C  
Detection: UV220nm  

Application Data

COSMOSIL Application Data

Column: COSMOGEL IEX Type M
Column size: 4.6mmI.D.-50mm
Mobile phase: A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3)
   + 0.5mol/l NaCl
B conc. 0→100% 30min Linear gradient
Flow rate: 0.5 ml/min
Temperature: 30°C
Detection: UV280nm
Sample: Urease (100µg)

COSMOSIL Application Data

Column: COSMOGEL IEX Type Q
Column size: 4.6mmI.D.-50mm
Mobile phase: A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3)
   + 0.5mol/l NaCl
B conc. 0→100% 30min Linear gradient
Flow rate: 0.5 ml/min
Temperature: 30°C
Detection: UV220nm
Sample: E.Coli Proteins
 

COSMOSIL Application Data

Column: COSMOGEL IEX Type S
Column size: 4.6mmI.D.-50mm
Mobile phase: A: 20mmol/l Phosphate buffer(pH6.8)
B: 20mmol/l Phosphate buffer(pH6.8)
   + 0.5mol/l NaCl
B conc. 0→100% 30min Linear gradient
Flow rate: 0.5 ml/min
Temperature: 30°C
Detection: UV220nm
Sample: Egg White

COSMOSIL Application Data

Column: COSMOGEL IEX Type Q-N
Column size: 4.6mmI.D.-30mm
Mobile phase: A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3)
   + 0.5mol/l NaCl
B conc. 10→60% 10min Linear gradient
Flow rate: 1.0 ml/min
Temperature: 30°C
Detection: UV220nm
Sample: Anti-IgG(H+L), Mouse, Goat-Poly,
Affinity purification
 

COSMOSIL Application Data

Column: COSMOGEL IEX Type Q-N
Column size: 4.6mmI.D.-100mm
Mobile phase: A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3)
   + 0.5mol/l NaCl
B conc. 10→20% 30min Linear gradient
Flow rate: 0.5 ml/min
Temperature: 30°C
Detection: UV220nm
Sample: Anti-GFP(Mouse IgG1-κ),
Monoclonal(GF200), AS (5.25µg)

COSMOSIL Application Data

Column: COSMOGEL IEX Type S-N
Column size: 4.6mmI.D.-100mm
Mobile phase: A: 20mmol/l Acetate buffer(pH4.5)
B: 20mmol/l Acetate buffer(pH4.5)
   + 0.5mol/l NaCl
B conc. 30→40% 30min Linear gradient
Flow rate: 0.5 ml/min
Temperature: 30°C
Detection: UV220nm
Sample: Anti-GFP(Mouse IgG1-κ),
Monoclonal(GF200), AS (5.25µg)
 

COSMOSIL Application Data

Column: COSMOGEL IEX Type Q-N
Column size: 4.6mmI.D.-100mm
Mobile phase: A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3)
   + 1.0mol/l NaCl
B conc. 75→85% 30min Linear gradient
Flow rate: 0.5 ml/min
Temperature: 30°C
Detection: UV260nm
Sample: DNA (100b.p.Ladder)

COSMOSIL Application Data

Column: COSMOGEL IEX Type Q-N
Column size: 4.6mmI.D.-100mm
Mobile phase: A: 20mmol/l Tris buffer(pH8.3)
B: 20mmol/l Tris buffer(pH8.3)
   + 1.0mol/l NaCl
B conc. 50→60% 30min Linear gradient
Flow rate: 0.5 ml/min
Temperature: 30°C
Detection: UV260nm
Sample: DNA (pd(A)40-60)
 

Ordering Information

Amphoteric ion-exchange
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOGEL IEX Type M Packed Column, 4.6 mm I.D. x 50 mm 06248-71 Waters type 1 pkg 1,700.00 Buy
COSMOGEL IEX Type M-N Packed Column, 4.6 mm I.D. x 100 mm 06244-11 Waters type 1 pkg 1,700.00 Buy
Anion-exchange
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOGEL IEX Type Q Packed Column4.6 mm I.D. x 50 mm 06266-31 Waters type 1 pkg 1,370.00 Buy
COSMOGEL IEX Type Q-N Packed Column, 4.6 mm I.D. x 30 mm 06264-51 Waters type 1 pkg 1,250.00 Buy
COSMOGEL IEX Type Q-N Packed Column, 4.6 mm I.D. x 100 mm 06258-41 Waters type 1 pkg 1,370.00 Buy
Cation-exchange
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOGEL IEX Type S Packed Column, 4.6 mm I.D. x 50 mm 06252-01 Waters type 1 pkg 1,370.00 Buy
COSMOGEL IEX Type S-N Packed Column, 4.6 mm I.D. x 30 mm 06251-11 Waters type 1 pkg 1,250.00 Buy
COSMOGEL IEX Type S-N Packed Column, 4.6 mm I.D. x 100 mm 06250-21 Waters type 1 pkg 1,370.00 Buy