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COSMOSIL Sugar-D

Saccharide Separation

Conventionally Aminopropyl bounded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional Aminopropyl bounded phases are tailing and adsorption of certain saccharides and general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL Sugar-D, resulting in better (sharper) separation and much improved durability. In addition COSMOSIL Sugar-D is useful in the separation of highly hydrophilic compounds which are not retained in conventional octadecyl (ODS) bounded stationary phases.

Certain types of saccharides such as Arabinose or Galactose are partially or temporarily absorbed in conventional Aminopropyl stationary phases causing disappearance of sample or tailing. These problematic saccharides elute sharply and in proportion of the injection volume from the superior COSMOSIL Sugar-D.

Durability

The decrease of retention time was compared between COSMOSIL Sugar-D and conventional Aminopropyl bounded stationary phase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case of COSMOSIL Sugar-D.

Eluent condition
Eluent
Flow rate
Temperature
: water
: 1.0 ml/min
: RT
Test condition
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample
: 4.6 mmI.D.×250 mm
: acetonitrile:water=70:30
: 1.0 ml/min
: 30°C
: RI
: Maltose

Analitical curve

Comparison

The separation performances and the adsorption of monosaccharides were compared in COSMOSIL Sugar-D and other companies' columns. The presence of Aldehyde-group in the sample saccharides (except of Fructose) does not result in undesirable adsorption, therefore has no ill effect on the performance of COSMOSIL Sugar-D column.

Oligomaltoses
Acidic Sugar

Application

Retention time
The conventional Aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5% - 10%.

Column size 4.6mmI.D.×250mm Flow rate 1.0ml/min Temperature: 30°C Detection: RI Sample 1: Glucose 2: Maltose

Separation of Saccharides
Mono- and Oligosaccharides Polyols

Cylclodextrin
Amino Sugar

Chromatogram

Condition
Column size
Mobile phase
Flow rate
Temperature
Detection
Sample
: 4.6 mmI.D.×250 mm
: acetonitrile:water=70:30
: 1.0 ml/min
: 30°C
: RI
1.Arabinose
2.Galactose
3.Maltitol

Ordering Information

Analytical (Particle Size : 5 µm)
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL Sugar-D Packed Column, 2.0 mm I.D. x 150 mm 05688-41 Waters type 1 pkg 440.00 Buy
COSMOSIL Sugar-D Packed Column, 2.0 mm I.D. x 250 mm 05689-31 Waters type 1 pkg 490.00 Buy
COSMOSIL Sugar-D Packed Column, 3.0 mm I.D. x 150 mm 05690-91 Waters type 1 pkg 440.00 Buy
COSMOSIL Sugar-D Packed Column, 3.0 mm I.D. x 250 mm 05691-81 Waters type 1 pkg 490.00 Buy
COSMOSIL Sugar-D Packed Column, 4.6 mm I.D. x 50 mm 07975-11 Waters type 1 pkg 380.00 Buy
COSMOSIL Sugar-D Packed Column, 4.6 mm I.D. x 150 mm 05395-71 Waters type 1 pkg 410.00 Buy
COSMOSIL Sugar-D Packed Column, 4.6 mm I.D. x 250 mm 05397-51 Waters type 1 pkg 490.00 Buy
Guard Column (Particle Size : 5 µm)
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL Sugar-D Guard Column, 4.6 mm I.D. x 10 mm 05394-81 Waters type 1 pkg 250.00 Buy
COSMOSIL Sugar-D Guard Column, 10.0 mm I.D. x 20 mm 05696-31 Waters type 1 pkg 560.00 Buy
COSMOSIL Sugar-D Guard Column, 20.0 mm I.D. x 50 mm 05694-51 Waters type 1 pkg 1,300.00 Buy
Preparative (Particle Size : 5 µm)
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL Sugar-D Packed Column, 10.0 mm I.D. x 250 mm 05692-71 Waters type 1 pkg 1,150.00 Buy
COSMOSIL Sugar-D Packed Column, 20.0 mm I.D. x 250 mm 05693-61 Waters type 1 pkg 2,420.00 Buy