Development of Sugar-D

Aldehyde groups in the open-chain form of sugars can form Schiff bases with the amino groups on aminopropyl columns, causing low recovery and peak tailing. COSMOSIL Sugar-D was designed to avoid this, so sugars like arabinose and galactose, which are problematic on aminopropyl columns, elute with sharp peaks. Sugar-D is especially useful for samples of low concentration.

Quantitation at low concentration

The decrease of retention time was compared between COSMOSIL Sugar-D and conventional Aminopropyl bounded stationary phase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case of COSMOSIL Sugar-D.

Eluent condition Eluent Flow rate Temperature : water : 1.0 ml/min : RT Test condition Column size Mobile phase Flow rate Temperature Detection Sample : 4.6 mmI.D.×250 mm : acetonitrile:water=70:30 : 1.0 ml/min : 30°C : RI : Maltose

The separation performances and the adsorption of monosaccharides were compared in COSMOSIL Sugar-D and other companies' columns. The presence of Aldehyde-group in the sample saccharides (except of Fructose) does not result in undesirable adsorption, therefore has no ill effect on the performance of COSMOSIL Sugar-D column.

Oligomaltoses
Acidic Sugar

We have compiled a list of our Sugar-D HPLC application data in an easy-to-read format. Please click the compound name to view its data.

Retention time
The conventional Aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5% - 10%.

Column size 4.6mmI.D.×250mm Flow rate 1.0ml/min Temperature: 30°C Detection: RI Sample 1: Glucose 2: Maltose

Separation of Saccharides
Mono- and Oligosaccharides Polyols

Cylclodextrin
Amino Sugar

Sugars in Sports Drinks (AP-0327)

Honey (AP-1530) (AP-1532) (AP-1533)

Sugar Alcohols (AP-0330)

Maltooligosaccharides (AP-0331)

Commercially Energy Drink (AP-1049)

Acarbose Analysis (PDF file)

Acarbose is used to treat type 2 diabetes. A recent study shows efficacy and tolerability of acarbose in patients with type 2 diabetes. By using COSOMSIL Sugar-D, which is designed for saccharide analysis, highresolution separation of Acarbose and its impurities was obtained. Furthermore, COSMOSIL Sugar-D was more durable than the conventional NH2 column.

Analysis of Sugars in Honey and Syrup (PDF file)

Analysis of honey and syrup is an important part of detecting food adulteration. Sugar-D provides good and consistent separation with high durability.

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size