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FG beads - Azide / Alkyne beads

The immobilization of the ligand by the click chemistry

Compounds or proteins  with azide or alkyne groups are immobilized on FG azide or alkyne beads easily by using a click chemistry reaction. 

                                                  Product information

Storage temperature 4℃ (2-8℃), Don't freezet
Storage buffer ultrapure water
Magnetization superparamagnetism(<10 emu/g)
Size of beads 190 nm±20 nm
Concentration 20 mg/ml
Functional groups Azide groups
Amounts of the functional groups 70-120 nmol/mg

Application; Target fishing

Affinity purification of target protein, DHRF (Dihydrofolate reductase), of MTX (methotrexate) with FG Azide and Alkyne beads.

1

Protocol

Chemical pulldown

E001 Screening by using ligand immobilized beads 259KB
E003 Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads 111KB
E004 Immobilization of ligands (carboxylic compounds) on OH beads 78KB
E005 Immobilization of ligands (carboxylic compounds) on NH2 beads 97KB
E008 Immobilization of ligands (compounds with NH2 groups) on COOH beads 139KB
E012 Competitive inhibition 261KB
E013 Drug elution 260KB
E014 Immobilization of ligands (compounds with NH2 groups) on NHS beads 75KB
E015 Immobilization of MTX aminated derivatives on NHS beads 66KB
E108 Immobilization of Ligand on Streptavidin beads 102KB
E109 Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction 65KB
E201 Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) 1,329KB

Immunoprecipitation, Protein-protein interaction

E011 Immunoprecipitation 77KB
E101 Immobilization of proteins on COOH beads 83KB
E102 Immobilization of His-Tag proteins on Ts beads 89KB
E105 Immobilization of Antibodies or Proteins on NHS beads 23KB
E106 Immobilization of Antibodies or Proteins on Epoxy Beads 65KB
E107 Direct Quantification of Immobilized Proteins (Antibodies) 107KB
E108 Immobilization of Ligand on Streptavidin beads 102KB
E110 Immobilization of antibodies on Protein A beads and Protein G beads 103KB

Purification of DNA and RNA binding substances

E001 Screening by using ligand immobilized beads 259KB
E301 Immobilization of double strand DNA on Plain beads 143KB

Ordering Information

FG beads
Product Cat.No. Storage Quantity Price(US$)  
Azide beads ** 50% OFF PROMOTION, List Price: $350 ** TAS8848 N1160 4°C 5 mg 175.00 Buy
Alkyne beads ** 50% OFF PROMOTION, List Price: $350 ** TAS8848 N1161 4°C 5 mg 175.00 Buy
Magnetic Stand
Product Cat.No. Storage Quantity Price(US$)  
Magnetic Stand (for 1.5 ml tube) ** 30% OFF PROMOTION, List Price: $800 ** TA4899N12 Room Temp. 1 ea 560.00 Buy
Magnetic Stand (for 15 ml tube) TA4899N20 Room Temp. 1 ea 600.00 Buy
Magnetic Stand (for 50 ml tube) TA4899N30 Room Temp. 1 ea 700.00 Buy