Recombinant Human Activin A, Animal-free
Introducing animal-free, recombinant Human Activin A expressed and purified using an Escherichia coli expression system, and manufactured by Ajinomoto Co. (Japan). Activin A is a member of the transforming growth factor β (TGF-β) family, and is used for the proliferation of pluripotent stem cells, as well as differentiation into endoderm and other different lineages.
This product is for research use only.
- Free from components of animal origin
- Frozen solution, easy to use
- High purity and activity
- High batch homogeneity
Comparison of product specification
Non-GMP product
| AJINOMOTO | Company A | Company B | Company C | |
|---|---|---|---|---|
| Host cell | E. coli | E. coli | CHO | Human |
| Endotoxin | <0.01EU/μg (LAL) |
<1EU/μg (LAL) |
<0.01EU/μg (LAL) |
<0.01EU/μg (LAL) |
| Purity | ≧97% (SDS-PAGE) |
≧97% (SDS-PAGE、HPLC) |
>95% (SDS-PAGE) |
>95% (SDS-PAGE) |
| Biological activity*1 |
ED50, 50-150% vs. WHO std*2 (K562) |
ED50, ≦2.0 ng/ml (MPC-11) |
ED50, 0.2-1.2 ng/ml (K562) |
ED50, 0.5-5 ng/ml (MPC-11) |
| Form | Frozen (0.1 mg/ml) |
Lyophilized | Lyophilized | Lyophilized |
| N-terminal | Gly-Leu-Glu | ー | Gly | ー |
| Expiration date | 4 years from manufacturing date |
ー | 1 year from date of receipt |
1 year from date of receipt |
- *1 Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
- *2 Activin A WHO international std., NIBSC code: 91/626
GMP compliant product
| AJINOMOTO | Company A | Company B | Company D | |
|---|---|---|---|---|
| Host cell | E. coli | E. coli | CHO | E. coli |
| Endotoxin | <0.01EU/μg (LAL) |
≦0.10EU/μg (LAL) |
<0.10EU/μg (LAL) |
<0.05EU/μg (LAL) |
| Purity | ≧97% (SDS-PAGE) |
≧98% (SDS-PAGE、HPLC) |
>97% (SDS-PAGE) |
≧97% (SDS-PAGE) *include ≦5% oligomers |
| Biological activity*1 |
ED50, 50-150% vs. WHO std*2 (K562) |
ED50, 1.0-5.0 ng/ml (MPC-11) |
ED50, 0.2-1.2 ng/ml (K562) |
0.6–2.5x103 IU/mg (MPC-11) |
| Mycoplasma | Negative (NAT) |
Negative (QF-PCR) |
Negative (Ribosomal RNA hybridization assay) |
ー |
| Sterility | Conform (Membrane filter) |
No growth (Direct inoculation) |
ー | No growth (Direct inoculation) |
| Form | Frozen (0.1 mg/ml) |
Lyophilized | Lyophilized | Lyophilized |
| N-terminal | Gly-Leu-Glu | Gly-Leu-Glu | Gly-Leu-Glu | Met-Gly-Leu-Glu |
| Expiration date | 4 years from manufacturing date |
5 years from manufacturing date |
3 years from bottling date |
Minimum 6 months from shipping date |
- *1 Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
- *2 Activin A WHO international std., NIBSC code: 91/626
Comparison of biological activity with other companies’ GMP products
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
Activin A WHO international std., NIBSC code: 91/626
AJINOMOTO’s Activin A demonstrates equal to superior biological activity compared to tested alternatives!
Comparison of MS spectrum with other companies’ GMP products
AJINOMOTO
Company X
Company Y
AJINOMOTO’s Activin A also contains significantly less impurities!
Stability information after reconstitution
| AJINOMOTO | Company A | Company B | |
| -80°C | 4 years from manufacturing date |
At least 7 months | 3 months |
| 4°C | At least 6 months | 1 week | 1 month |
| Freeze-thaw cycles | Within 2 times (>90% activity) |
ー | ー |
Product Information
Recombinant Human Activin A (10 µg) (Product Information)Ordering Information
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| Recombinant Human Activin A (#SP-ActA-R-010UG) | activin-10 | -80°C | 10 µg | 185.00 | Buy |
| Recombinant Human Activin A (#SP-ActA-R-050UG) | activin-50 | -80°C | 50 µg | 775.00 | Buy |
| Recombinant Human Activin A (#SP-ActA-R-001MG) | activin-1000 | -80°C | 1 mg | 5,835.00 | Buy |
| Recombinant Human Activin A (GMP grade) (#SP-ActA-G-001MG) | activin-GMP | -80°C | 1 mg | - | Inquire |
Shipping with ice pack within the US