Stripping buffer solution
WB Stripping Solution removes conjugated antibodies from blots, enabling subsequent detections with different antibodis on the very same blot. After the first antigen-antibody reaction and following chemiluminescent visualization, the antibody can be removed by the WB Stripping Solution. A second antigen-antibody reaction can be conducted on the same blot. The same blot can be probed 2-5 times if chemiluminescent detection is employed. Alternatively, one blot can be used multiple times under different conditions to optimise the result of antigen-antibody reaction.
|No heating:||reaction at room temperature|
|No odor:||does not contain 2-mercaptoethanol|
|Fast:||stripping time 5-15 minutes|
|Ready to use:||one solution in one bottle|
Data courtesy of Akaike Lab, Tokyo Institute of Technology
Chemiluminescence detection with Chemi-Lumi One L
If antibody remains on the membrane, it can be detected after the new antigen-antibody reaction causing false result.