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WB Stripping Solution

Stripping buffer solution

WB Stripping Solution removes conjugated antibodies from blots, enabling subsequent detections with different antibodis on the very same blot. After the first antigen-antibody reaction and following chemiluminescent visualization, the antibody can be removed by the WB Stripping Solution. A second antigen-antibody reaction can be conducted on the same blot. The same blot can be probed 2-5 times if chemiluminescent detection is employed. Alternatively, one blot can be used multiple times under different conditions to optimise the result of antigen-antibody reaction.

No heating: reaction at room temperature
No odor: does not contain 2-mercaptoethanol
Fast: stripping time 5-15 minutes
Ready to use:   one solution in one bottle

Application 1

1. First antigen-antibody reaction
   Blocking        : Blocking One (30 min)  (code No.: 03953-95)
   Wash            :  t-Tris Buffered Saline
   Primary ab      : anti-paxillin (mouse IgG)
   Secondary ab  : anti-mouse IgG-POD
   Detection       : Chemiluminescence Detection Kit (commercially available product)

2. Stripping
   Solution         : WB Stripping Solution
   Condition        : RT, 15 min for conventional protocol

3. Second, different antiogen-antibody reaction
   Blocking         : Blocking One (30 min) (code No.: 03953-95)
   Wash              :  t-Tris Buffered Saline
   Primary ab       : anti-vinculine (mouse IgG)
                       : anti-actin (mouse IgG)
   Secondary ab   : anti-mouse IgG-POD
   Detection        : Chemiluminescence Detection Kit

Data courtesy of Akaike Lab, Tokyo Institute of Technology

Application 2

Chemiluminescence detection with Chemi-Lumi One L

Note

  • Depending on the strength of the antigen-antibody reaction, the antibody complex may remain on the membrane after 5-15 minutes stripping treatment at room temperature. It is recommended to optimize the conditions beforehand. If antibodys remain, it is recommended to extend the stripping time (30 minutes to several hours) and/or raise temperature (37 to 60 oC). Perform blocking again if necessary.
  • The product is an acidic solution (pH 2-3). Wear protective chemical-resistant clothes, gloves, and safety eye-wears. If the product accidentally comes into contact with skin, thoroughly wash the skin with water. Contact a physician if necessary.
  • Use the product after it has been equilibrated at room temperature.
  • This product is not suitable for use for blotting membrane stained with TMB, DAB, 4-chloronaphtol or other visually detectable staining, because these stains will not disappear from the membrane.
  • After use, the product shall be neutralized and discarded.
  • If the product is mixed with WB Stripping Solution Strong (Code No.:05677-65), a white precipitation will occur. When both products are used in turns, the blotting membrane must be washed throughly with a proper buffer or deionized water for 3-5 times. Use separate trays for different reagents.

Proof of Antibody Removal

If antibody remains on the membrane, it can be detected after the new antigen-antibody reaction causing false result.

  • Check whether any labeled antibody complex remains after the stripping by repeated chemiluminescence detection.
  • Check whether any non-labeled (primary) antibody remains by chemiluminescence detection upon repeated reaction with the secondary antibody

Difference between WB Stripping Solution and WB Stripping Solution Strong

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
WB Stripping Solution 05364-55 Room Temp. 500 ml 98.00 Buy
WB Stripping Solution Strong 05677-65 4ーC 500 ml 118.00 Buy
WB Stripping Solution Trial Set (WB Stripping Solution + WB Stripping Solution Strong) 05680-21 4ーC 40 ml each 30.00 Buy