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3α-Hydroxysteroid Dehydrogenase

Others

3α-Hydroxysteroid: NAD(P)+ Oxidoreductase [EC 1.1.1.50]

Origin

Pseudomonas testosteroni

Reaction

3Α-hydroxysteroid + NAD(P)+ 3-Oxosteroid + NAD(P)H+H+

Appearance

White amorphous powder.

Activity

More than 90 units/mg protein.

Unit definition

One unit is the amount of enzyme required to oxidize 1 ?mol of Androsterone as a substrate per minute in the presence of NAD at pH 8.9 at 25°C.

Storage

Stable for one year when stored below 5°C and also stable at room temperature for at least one week. For prolonged storage, keep at -20°C.

Contaminants

Malate Dehydrogenase < 0.01%
Lactate Dehydrogenase < 0.01%
Alcohol Dehydrogenase < 0.01%
β-Hydroxysteroid dehydrogenase < 0.5 %

Properties

Molecular weight --- Approx. 37,000 Da
Optimum pH --- 10.2 ~ 10.5
Optimum temperature --- 50°C
pH stability --- 6.0 ~ 9.5 (30°C, 17 hr)
Thermal stability --- below 50°C (pH 7.2, 10 min)
Michaelis constant --- 6.7x10-6 M (Androsterone)
8.3x10-6 M (Na-cholate)
6.7x10-5 M (NAD)

Uses

Determination of bile acid

Ordering Information

Sialic Acid Related Products - Enzymes
Product Cat.No. Storage Quantity Price(US$)  
3α-Hydroxysteroid dehydrogenase 3α-Hydroxysteroid : NAD(P)+ oxidoreductase [EC 1.1.1.50] 18949-34 -20°C 10 units 140.00 Buy
3α-Hydroxysteroid dehydrogenase 3α-Hydroxysteroid : NAD(P)+ oxidoreductase [EC 1.1.1.50] 18949-76 -20°C 50 units 320.00 Buy