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β-Hydroxysteroid Dehydrogenase

Others

3(or 17)β-Hydroxysteroid:NAD(P)+ Oxidoreductase [EC 1.1.1.51]

Origin

Pseudomonas testosteroni

Reaction

(1) epi-androsterone + NAD(P)+ 5α-Androstene-3,17-dione + NAD(P)H+H+
(2) testosterone + NAD(P)+ 4-Androstene-3,17-dione + NAD(P)H+H+

Appearance

White amorphous powder.

Activity

More than 25 units/mg protein.

Unit definition

One unit oxidize 1 µmol of testosterone as a substrate per minute in the presence of NAD at pH 8.9 at 25°C.

Contaminants

Malate Dehydrogenase < 0.01%
Lactate Dehydrogenase < 0.01%
Alcohol Dehydrogenase < 0.01%
3α-Hydroxysteroid Dehydrogenase approx. 0.1 %

Storage

Stable for one year when stored below 5°C and also stable at room temperature for at least one week. For prolonged storage, keep at -20°C.

Properties

Molecular weight --- Approx. 80,000 Da
Optimum pH --- 10.1 ~ 10.3
Optimum temperature --- 34°C
pH stability --- 7.0 ~ 8.7
Thermal stability --- below 42°C (pH 7.2, 10 min)
Michaelis constant --- 2.5x10-6 M (Testosterone)
1.8x10-4 M (NAD)

Ordering Information

Sialic Acid Related Products - Enzymes
Product Cat.No. Storage Quantity Price(US$)  
β-Hydroxysteroid dehydrogenase 3(or17) β-Hydroxysteroid : NAD(P)+ oxidoreductase [EC 1.1.1.51] 18950-94 -20°C 10 units 97.00 Buy