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Neuraminidase [Sialidase] [EC 3.2.1.18]

Sialic Acid Related Products

Origin

Arthrobacter ureafaciens

Reaction

Sialyl compound → Sialic Acid + Asialocompound

Appearance

White amorphous powder.

Activity

More than 80 units/mg protein for N-Acetylneuraminyllactose (NANA-lactose).

Unit definition

One unit is the amount of enzyme required to liberate 1 µmol of N-Acetylneuraminic Acid (NANA) per minute at pH 5.0 at 37°C.

Storage

Stable for one year when stored below 5°C. For prolonged storage, keep at -20°C.

Contaminant

Enzyme activities mentioned below cannot be detected. Protease , N-Acetylneuraminic Acid Aldolase, Glycosidase such as α-Glucosidase, β-Glucosidase, α-Galactosidase, β-Galactosidase, α-Mannosidase, α-Fucosidase, N-Acetyl-α-glucosaminidase, N-Acetyl-β-glucosaminidase, N-Acetyl-agalactosaminidase, N-Acetyl-β-galactosaminidase, N-Acetyl-α-mannosaminidase and N-Acetyl-β-mannosaminidase.

Properties

Molecular weight --- Approx. 52,000 Da, 66,000 Da and 88,000 Da (gel filtration, SDS-PAGE)
Optimum pH --- 4.5 ~ 5.5 (NANA-lactose as a substrate)
pH stability --- 4.5 ~ 9.5
Thermal stability --- below 60°C (pH 5.0, 20 min)
Substrate specificity --- The α-ketosidic linkage of N-Glycolylneuraminic Acid (NGNA) can be hydrolyzed as well as that of NANA. This enzyme cleaves α(2 → 3), α(2 → 6) and α(2 → 8) linkages of N-Acetylneuraminic Acid in glycoconjugates. The acivity is independent on Ca2+ and is not inhibited by EDTA, which is in striking contrast to Vibrio cholerae Neuraminidase, and is not or slightly inhibited by inhibitors such as Monoiodoacetate, p-Chloromercuribenzoate and HgCl2, which is in striking contrast to Clostridium perfringens Neuraminidase.

Ordering Information

Sialic Acid Related Products - Enzymes
Product Cat.No. Storage Quantity Price(US$)  
Neuraminidase [Sialidase] Acylneuraminyl hydrolase [EC 3.2.1.18] 24229-61 -20°C 1 unit 79.00 Buy
Neuraminidase [Sialidase] Acylneuraminyl hydrolase [EC 3.2.1.18] 24229-74 -20°C 5 units 270.00 Buy