Sialic Acid Related Products
Molecular weight | --- | Approx. 52,000 Da (gel filtration, SDS-PAGE) |
Optimum pH | --- | 3.8 ~ 4.4 ( for bovine brain ganglioside ) |
pH stability | --- | 3.5 ~ 10.0 |
Thermal stability | --- | below 60°C (pH 5.0, 20 min) |
Substrate specificity | --- | Isozyme S cleaves α(2 → 3), α(2 → 6) and α(2 → 8) linkages of N-Acetylneuraminic Acid in glycoconjugates. In the absence of detergents and calcium ion, the enzyme hydrolyzes N-Acetylneuraminosyl moiety of polysialogangliosides and produces monosialoganglioside GM1, while in the presence of detergents ( Na-cholate, Triton X-100 etc. ), GM1 is further desialylated to asialoganglioside GA1. the character of the enzyme is similar to Vibrio cholerae Neuraminidase except requirement of Ca2+ in the latter. |