Nacalai USA - Innovations for Life Sciences

Subtotal: $0.00

View Cart

Protease Inhibitor Cocktail (EDTA free)

EDTA free

Inhibition of intra and extra cellular proteases is vital to purify and collect the expressed proteins. Saving trouble of finding adequate inhibitors, a wide range of proteases are inhibited by the Protease Inhibitor Cocktail. This product does not interfere with metalloproteins since it is EDTA free.

  • The product does not contain phosphorylated proteins.
  • The product is phosphatase free.
  • The preservative in the product does not affect the enzyme activity of horseradish peroxidase (HRP).
  • Store the product in refrigerator after opening.

Wide range: Contains inhibitors for a variety of serin and cysteine proteases
Contain no EDTA: efficient for purification of metalloproteins and His tag gene exchanged proteins
Easy to use: dilute according to your need (100X stock concentration)

Enzyme inhibition efficiency
Origin of ProteaseProteaseProtease Inhibitor Cocktail (EDTA free)
(Protease concentration)
Papain
(from papaya)
Cystein 100%
(2.5 µg/ml)
96.1%
(5.0 µg/ml)
96.5%
(20 µg/ml)
Trypsin
(from porcine pancreas)
Serine 96.9%
(2.0 µg/ml)
94.0%
(4.0 µg/ml)
88.1%
(8.0 µg/ml)
Chymotrypsin
(from bovine pancreas)
Serine 95.1%
(0.75 µg/ml)
90.5%
(1.5 µg/ml)
83.1%
(3.0 µg/ml)
Pronase
(from Streptomyces griseus)
Mixture 96.5%
(0.6 µg/ml)
95.3%
(1.2 µg/ml)
92.3%
(2.4 µg/ml)
Pancreas extract
(bovine)
Mixture 89.8%
(5.0 µg/ml)
87.2%
(10 µg/ml)
85.6%
(20 µg/ml)

Composition

The product (100x stock solution) contains:
Inhibitors Targeted proteasee
50 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) Serine protease
15 µmol/l Aprotinin Serine protease, elastase
100 µmol/l E-64 Cystein protease
100 µmol/l Leupeptin hemisulfate monohydrate Cystein protease, trypsin like protease

Protocol

  1. Dissolve the powdered product by adding 1 ml deionized water into the bottle to make the 100 times concentrated stock solution.
  2. Dilute with your choice of buffer to make 100 ml of 1X protease inhibitor solution.

Note

  • In case of very high protease concentration, use higher concentration of the product (dilute only up to between 50 and 20 times).
  • Keep the inhibitor cocktail stock solution at -20? or lower, and use it within 3 months.

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Protease Inhibitor Cocktail (EDTA free) (100X) Lyophilized Powder 03969-21 -20 °C 5 x 1 ml 149.00 Buy
Protease Inhibitor Cocktail (EDTA free) (100X) Lyophilized Powder 03969-34 -20 °C 15 x 1 ml 410.00 Buy