Anti-DYKDDDDK (Mouse) Monoclonal
DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. Anti-DYKDDDDK (Mouse IgG2b-κ) is a high-affinity monoclonal antibody that can binds to same epitope as Sigma's Anti-FLAG® M2 antibody.
Anti-DYKDDDDK (Mouse IgG2b-κ), Monoclonal (5A8E5)
| Clone | : | 5A8E5 |
| Isotype | : | IgG2b-κ (Mouse) |
| Product form | : | Lyophilized form |
| Specificity | : | Anti-DYKDDDDK (Mouse IgG2b-κ) recognizes C-terminal, N-terminal and internal tagged fusion proteins. |
| Concentration | : | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Immunogen | : | A synthetic peptide (DYKDDDDK) coupled KLH |
| Application | : | Western Blotting 0.1-1.0 µg/ml Immunoprecipitation 1 µg/ml Immunofluorescent 1 µg/ml ELISA 0.05-0.2 µg/ml |
Anti-DYKDDDDK (Mouse), Monoclonal (6H8E4), Agarose Conjugate
| Clone | : | 6H8E4 |
| Degree of substitution | : | Approx. 8.5 mg Anti-DYKDDDDK mAb / ml settled resin |
| Product form | : | 1x PBS containing 50% glycerol |
Comparison of Anti-DYKDDDDK (Mouse IgG2b-κ) (#NU01102) with Mouse Anti-DYKDDDDK Tag mAb (Company S, clone M2, 1 µg/ml) by Western blot
| Sample | : | Lane 1. N-terminal DYKDDDDK-tagged fusion protein Lane 2. Internal DYKDDDDK-tagged fusion protein Lane 3. C-terminal DYKDDDDK-tagged fusion protein |
| Primary antibody | : | (A) Anti-DYKDDDDK (Mouse IgG2b-κ), Monoclonal (5A8E5), 1 mg/ml (B) Company S, Anti-DYKDDDDK Tag mAb (M2), 1 mg/ml |
| Secondary antibody | : | Goat anti-Mouse IgG IRDye™ 800 Conjugated |
| Sample | : | Lane 1-3 Multiple Tag (Purified) (400 ng, 80 ng,16 ng) Lane 4. 293 cell lysate 10 m |
| Primary antibody | : | (A) Anti-DYKDDDDK (Mouse IgG2b-κ), Monoclonal (5A8E5), 1 mg/ml (B) Company S, Anti-DYKDDDDK Tag mAb (M2), 1 mg/ml |
| Secondary antibody | : | Goat anti-Mouse IgG IRDye™ 800 Conjugated |
Consistent analysis of Batch 1# and Batch 2# of Anti-DYKDDDDK (Mouse IgG2b-κ) by Western Blotting
The figure shows that signal remains consistent from Lot to Lot.
The assay was performed with DYKDDDDK-tagged fusion protein.
The signal was developed with Goat-anti-Mouse IgG IRDye™ 800 conjugated.
Western Blotting analysis of E. coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecopitates
Lane 1.
E.coli lysate containing DYKDDDDK-tagged fusion protein
Lane 2.
Immunoprecipitates of free E.coli lysate incubated with Anti-DYKDDDDK (Mouse IgG2b-κ) (2 µg/ml) and Protein A.
Lane 3
Immunoprecipitates of E.coli lysate containing DYKDDDDK-tagged fusion protein incubated with Anti-DYKDDDDK (Mouse IgG2b-κ) (2 µg/ml) and Protein A.
Immunoprecipitation: Anti-DYKDDDDK (Mouse), Agarose Conjugate
Immunoprecipitation-Western Blotting analysis of DYKDDDDK tagged fusion protein
Lane 1. DYKDDDDK tagged fusion protein input control
Lane 2. Anti-DYKDDDDK, Agarose Conjugate
Lane 3. DYKDDDDK tagged fusion protein was precipitated by Anti-DYKDDDDK, agarose conjugate.
Epitope Tag Antibodies (Brochure)