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Chemi-Lumi One Ultra: Chemiluminescent Reagents for Western Blotting

Low-femtogram Sensitivity Substrate for HRP / The Highest Sensitivity

Features

  • Low-femtogram sensitivity
  • Longer signal duration

Comparison of Sensitivity with SuperSigmal West Femto

Chemi-Lumi One Ultra is an extremely sensitive chemiluminescent substrate. Western blot of identical transferrin samples. The membranes were incubated with substrate that was prepared according to the manufacturers' instructions. The membranes were exposed to film for 2 minutes.                                   
  - Primary antibody : Rabbit anti-transferrin, 1: 30,000
  - Secondary antibody: Goat anti-rabbit IgG-HRP, 1: 200,000

Comparison of Sensitivity with Competitors

Chemi-Lumi One Ultra shows equal to or higher sensitivity compared to competitors'

Gel: 10% PAGE gel
Wash: 0.1% t-TBS (x1), pH 7.4
Blocking: Blocking One (Cat. No.: 03953)
1st Ab: Anti-β-Actin Mouse Monoclonal Antibody (Santa Cruz, Cat. No.: sc-47778), 1:300
2nd Ab: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Crus, Cat. No.: sc-2005), 1: 1000
Detection Period: 5 min.
Detector: LAS-3000 High Mode
Expose Time: 3 min.

Comparison of Abs Concentration

Due to low background, Chemi-Lumi One Ultra is applicable to wide range of primary antibodies, and makes it easy to optimize condition.

Gel: 10% PAGE gel
Wash: 0.1% t-TBS (x1), pH 7.4
Blocking: Blocking One (Cat. No.: 03953)
1st Ab: Anti-PCNA Mouse Monoclonal Antibody (Santa Cruz, Cat. No.: sc-56c)
2nd Ab: Anti-Mouse IgG (Goat), HRP Conjugated (Santa Crus, Cat. No.: sc-2005), 1: 100,000
Detection Period: 5 min.
Detector: LAS-3000 High Mode
Expose Time: 1:1000, 3 min. / 1:5000, 5 min. / 1:10,000, 8 min.

Reference

  1. Iwasaki, Takeshi, et al. "Establishment and Characterization of a Novel Primitive Yolk Sac Tumour Cell Line, TC587." Anticancer research 40.2 (2020): 759-766.
  2. Saito, Akatsuki, et al. "The 4th and 112th residues of viral capsid cooperatively modulate capsid-CPSF6 interactions of HIV-1." AIDS Research and Human Retroviruses ja (2020).
  3. Kitakaze, Tomoya, et al. "Extracellular transglutaminase 2 induces myotube hypertrophy through G protein-coupled receptor 56." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1867.2 (2020): 118563.
  4. Dang-Nguyen, Thanh Quang, et al. "Combined refinements to somatic cell nuclear transfer methods improve porcine embryo development." Journal of Reproduction and Development (2020): 2019-156.
  5. Haraguchi, Seiki, et al. "Establishment of porcine nuclear transfer-derived embryonic stem cells using induced pluripotent stem cells as donor nuclei." Journal of Reproduction and Development (2020): 2019-137.
  6. Chen, Chen, et al. "Isolation of a novel bacterial strain capable of producing abundant extracellular membrane vesicles carrying a single major cargo protein and analysis of its transport mechanism." Frontiers in Microbiology 10 (2020): 3001.
  7. Noguchi, Taiki, et al. "Regulatory expression of bone morphogenetic protein 6 by 2, 2′-dipyridyl." Biochimica et Biophysica Acta (BBA)-General Subjects (2020): 129610.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Chemi-Lumi One Ultra (Luminol 50 mL, Peroxide 50 mL, Sufficient substrate for 1,000 cm² of blotting membrane) 11644-40 4°C 1 Kit 298.00 Buy
Chemi-Lumi One Ultra (Luminol 10 mL, Peroxide 10 mL, Sufficient substrate for 200 cm² of blotting membrane) 11644-24 4°C 1 Kit 110.00 Buy