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FG beads HM- Protein A / Protein G beads

IgG purification / Immunoprecipitation (IP) / Chromatin Immunoprecipitation (ChIP) / Protein separation

     Product information

  Protein A Protein G
Storage temperature 4℃ (2-8℃), Don't freeze
Storage buffer 10mM HEPES-NaOH (pH7.9) 10mM HEPES-NaOH (pH7.9)
Size of beads 140 nm±20 nm
Concentration 20 mg/ml
Functional groups Protein A Protein G
Capacity >25 ug Human IgG/mg of beads >25 ug Human IgG/mg of beads

 

Features

  • High recovery
    IgG binding capacity - more than twice the amount of a competitor.
  • High purity
    Extremely low non-specific adsorption.
  • Quick processing
    30 minutes for IgG binding.

Applications

  • IgG purification
  • Immunoprecipitation (IP)
  • Chromatin Immunoprecipitation (ChIP)
  • Protein separation

IgG Purification

We compared the binding capacity of FG beads to capture IgG in HeLa cell extracts with the beads of a competitor. FG beads captured larger amounts of IgG than the competitor's beads.

  1. Add 10 µg of IgG into 200 µg of HeLa cell extracts (200 µl).
  2. Add 0.3 mg of each beads to the HeLa cell extracts.
  3. React for 10 min at 4°C and separate beads from the HeLa cell extract.
  4. Elute bound IgG by adding Glycine-HCl.

Immunoprecipitation

We checked the performance of FG beads (Protein A and Protein G) in an immunoprecipitation experiment. By using FG beads, antigen HSA was immunoprecipitated with high recovery and extremely low non-specific adsorption.

  1. Immobilize anti-Human Serum Albumin antibody on FG beads.
  2. Add 400 ng of HSA into 200 µg of HeLa cell extracts (200 µl).
  3. Add 0.1 mg of each beads to HeLa cell extracts.
  4. React for 120 min at 4°C and separate beads from the HeLa cell extract.
  5. Elute bound IgG and HSA by adding Glycine-HCl.

Ordering Information

FG beads HM
Product Cat.No. Storage PKG Size Price(US$)  
HM-Protein A beads TAB8848N3172 4°C 5 mg 175.00 Buy
HM-Protein G beads TAB8848N3173 4°C 5 mg 175.00 Buy