IgG purification / Immunoprecipitation (IP) / Chromatin Immunoprecipitation (ChIP) / Protein separation
|Protein A||Protein G|
|Storage temperature||4℃ (2-8℃), Don't freeze|
|Storage buffer||10mM HEPES-NaOH (pH7.9)||10mM HEPES-NaOH (pH7.9)|
|Size of beads||140 nm±20 nm|
|Functional groups||Protein A||Protein G|
|Capacity||>25 ug Human IgG/mg of beads||>25 ug Human IgG/mg of beads|
We compared the binding capacity of FG beads to capture IgG in HeLa cell extracts with the beads of a competitor. FG beads captured larger amounts of IgG than the competitor's beads.
We checked the performance of FG beads (Protein A and Protein G) in an immunoprecipitation experiment. By using FG beads, antigen HSA was immunoprecipitated with high recovery and extremely low non-specific adsorption.