FG beads HM- Protein A / Protein G beads
IgG purification / Immunoprecipitation (IP) / Chromatin Immunoprecipitation (ChIP) / Protein separation
Product information
| Protein A | Protein G | |
| Storage temperature | 4℃ (2-8℃), Don't freeze | |
| Storage buffer | 10mM HEPES-NaOH (pH7.9) | 10mM HEPES-NaOH (pH7.9) |
| Size of beads | 140 nm±20 nm | |
| Concentration | 20 mg/ml | |
| Functional groups | Protein A | Protein G |
| Capacity | >25 ug Human IgG/mg of beads | >25 ug Human IgG/mg of beads |
Features
- High recovery
IgG binding capacity - more than twice the amount of a competitor. - High purity
Extremely low non-specific adsorption. - Quick processing
30 minutes for IgG binding.
Applications
- IgG purification
- Immunoprecipitation (IP)
- Chromatin Immunoprecipitation (ChIP)
- Protein separation
IgG Purification
We compared the binding capacity of FG beads to capture IgG in HeLa cell extracts with the beads of a competitor. FG beads captured larger amounts of IgG than the competitor's beads.
- Add 10 µg of IgG into 200 µg of HeLa cell extracts (200 µl).
- Add 0.3 mg of each beads to the HeLa cell extracts.
- React for 10 min at 4°C and separate beads from the HeLa cell extract.
- Elute bound IgG by adding Glycine-HCl.
Immunoprecipitation
We checked the performance of FG beads (Protein A and Protein G) in an immunoprecipitation experiment. By using FG beads, antigen HSA was immunoprecipitated with high recovery and extremely low non-specific adsorption.
- Immobilize anti-Human Serum Albumin antibody on FG beads.
- Add 400 ng of HSA into 200 µg of HeLa cell extracts (200 µl).
- Add 0.1 mg of each beads to HeLa cell extracts.
- React for 120 min at 4°C and separate beads from the HeLa cell extract.
- Elute bound IgG and HSA by adding Glycine-HCl.