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FG beads HM - Streptavidin / NeutrAvidin™ beads

Immunoprecipitation (IP) / Chromatin Immunoprecipitation (ChIP) / Cell separation / Affinity purification of drug target proteins

     Product information

  Streptavidin NeutrAvidin
Storage temperature 4℃ (2-8℃), Don't freeze
Storage buffer 10mM HEPES-NaOH (pH7.9) 10mM HEPES-NaOH (pH7.9)
Size of beads 140 nm±20 nm
Concentration 20 mg/ml
Functional groups Streptavidin NeutrAvidin
Capacity >3 ug biotinylated BSA/mg of beads >3 ug biotinylated BSA/mg of beads

 

Features

  • High recovery
    Biotin binding capacity - more than twice the amount of a competitor.
  • High purity
    Extremely low non-specific adsorption.

Applications

  • Immunoprecipitation (IP)
  • Chromatin Immunoprecipitation (ChIP)
  • Cell separation
  • Affinity purification of drug target proteins

Immunoprecipitation

We compared the performance of FG beads in an immunoprecipitation experiment with the beads of a competitor. By using FG beads, antigen HSA was immunoprecipitated with high recovery and extremely low non-specific adsorption.

  1. Immobolize biotinylated anti-HSA antibody on Streptavidin beads.
  2. Add 1 µg of HSA into 600 µg of HeLa cell extracts (200 µl).
  3. Add 0.5 mg of each beads to the HeLa cell extracts.
  4. React for 60 min at 4°C and separate beads from the HeLa cell extract.
  5. Elute bound IgG and HSA by adding Glycine-HCl.

Affinity Purification of Drug Target Protein

We compared the performance of biotinylated drug MTX (Methotrexate) immbilized FG beads with the beads of a competitor in a target protein purification expertiment. By using FG beads, MTX target protein DHFR was purified with extremely low non-specific adsorption.

  1. Immobolize biotinylated MTX on Streptavidin beads.
  2. Add 0.5 mg of each beads into 600 µg of HeLa cell extracts (200 µl).
  3. React for 120 min at 4°C and separate beads from the HeLa cell extract.
  4. Elute bound DHFR by adding elution buffer.

Ordering Information

FG beads HM
Product Cat.No. Storage PKG Size Price(US$)  
HM-Streptavidin beads TAB8848N3170 4°C 5 mg 400.00 Buy
HM-NeutrAvidin™ beads TAB8848N3171 4°C 5 mg 400.00 Buy