COSMOSIL HP (3-Hydroxyphenyl)
COSMOSIL SFC COLUMNS / 3-Hydroxyphenyl group
Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations.
| COSMOSIL 5HP (3-Hydroxyphenyl) | |
|---|---|
| Silicagel | high purity porous spherical silica |
| Average particle size | 2.5, 3 and 5µm |
| Average pore size | approx. 120Å |
| Specific surface area | approx. 300m2/g |
Application 1: Non-steroidal anti-inflammatory drugs
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Carbon Dioxide : Methanol |
| Gradient: | 95:5 to 65:35 over 6 minutes |
| Flow rate: | 5.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV230nm |
| Sample: | 1: Ibuprofen 2: Fenoprofen 3: Flurbiprofen 4: Ketoprofen 5: Indoprofen |
Application 2: Beta Blockers
Peak elution order reversal under identical Conditions
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | Carbon Dioxide : Methanol w/20mM ammonium formate |
| Gradient: | 95:5 to 50:50 over 4 minutes |
| Flow rate: | 5.0 ml/min |
| Temperature: | 30°C |
| Detection: | APCI(+) |
| Sample: | 1. Pindolol 2. Atenolol |
Comparison of Retention Behavior
The following three stationary phases were evaluated for their retention of hydrophilic, hydrophobic, acidic and basic compounds. COSMOSIL HP and PY elute hydrophobic compounds first and retain hydrophilic compounds longer, whereas PBr elutes in the reverse order, exhibiting high retention for hydrophobic compounds. HP had the longest retention for basic compounds.
| Column size: | 4.6 mmI.D. x 150 mm |
| Mobile phase: | A: CO2 B: 0.1% CH3COONH4 - Methanol B conc. 0→50% (0→14min), 50% (14-17min) |
| Flow rate: | 0.8 ml/min |
| BPR | 10 MPa |
| Temperature: | 40°C |
| Detection: | ESI-MS/MS, MRM |
| Sample |
(No.1) Uridine |
| Sample conc. | 8 µmol/l |
| Inj. Vol | 2.0 µl |
Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics
Ordering Information
Particle Size: 2.5µm
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column (3.0 mm I.D. x 50 mm) | NU05002 | 1 pkg | N/A | Inquire |
| COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column (3.0 mm I.D. x 100 mm) | NU05005 | 1 pkg | N/A | Inquire |
| COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column (3.0 mm I.D. x 150 mm) | NU05006 | 1 pkg | N/A | Inquire |
Particle Size: 3µm
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOSIL 3HP (3-Hydroxyphenyl) Packed Column (2.0 mm I.D. x 150 mm) | 13792-11 | 1 pkg | 835.00 | Buy |
| COSMOSIL 3HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 250 mm) | 13793-01 | 1 pkg | 885.00 | Buy |
Particle Size: 5µm
| Product | Cat.No. | PKG Size | Price(US$) | |
|---|---|---|---|---|
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (2.0 mm I.D. x 150 mm) | 13787-91 | 1 pkg | 775.00 | Buy |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 100 mm) | 13971-61 | 1 pkg | 600.00 | Buy |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 150 mm) | 13972-51 | 1 pkg | 610.00 | Buy |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 250 mm) | 13788-81 | 1 pkg | 775.00 | Buy |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (10.0 mm I.D. x 150 mm) | 13973-41 | 1 pkg | 1,325.00 | Buy |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (10.0 mm I.D. x 250 mm) | 13789-71 | 1 pkg | 1,765.00 | Buy |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (20.0 mm I.D. x 150 mm) | 13855-91 | 1 pkg | N/A | Inquire |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (20.0 mm I.D. x 250 mm) | 13790-31 | 1 pkg | N/A | Inquire |
| COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (10.0 mm I.D. x 20 mm) | 13791-21 | 1 pkg | 660.00 | Buy |