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COSMOSIL HP (3-Hydroxyphenyl)

COSMOSIL SFC COLUMNS / 3-Hydroxyphenyl group

Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations.

COSMOSIL 5HP (3-Hydroxyphenyl)
Silicagel high purity porous spherical silica
Average particle size 2.5, 3 and 5µm
Average pore size approx. 120Å
Specific surface area approx. 300m2/g

Application 1: Non-steroidal anti-inflammatory drugs

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Carbon Dioxide : Methanol
Gradient: 95:5 to 65:35 over 6 minutes
Flow rate: 5.0 ml/min
Temperature: 30°C
Detection: UV230nm
Sample: 1: Ibuprofen
2: Fenoprofen
3: Flurbiprofen
4: Ketoprofen
5: Indoprofen

 

Application 2: Beta Blockers

Peak elution order reversal under identical Conditions

Column size: 4.6 mmI.D. x 150 mm
Mobile phase: Carbon Dioxide : Methanol w/20mM ammonium formate
Gradient: 95:5 to 50:50 over 4 minutes
Flow rate: 5.0 ml/min
Temperature: 30°C
Detection: APCI(+)
Sample: 1. Pindolol
2. Atenolol

Comparison of Retention Behavior

The following three stationary phases were evaluated for their retention of hydrophilic, hydrophobic, acidic and basic compounds. COSMOSIL HP and PY elute hydrophobic compounds first and retain hydrophilic compounds longer, whereas PBr elutes in the reverse order, exhibiting high retention for hydrophobic compounds. HP had the longest retention for basic compounds.

Column size: 4.6 mmI.D. x 150 mm
Mobile phase:   A: CO2
B: 0.1% CH3COONH4 - Methanol
B conc. 0→50% (0→14min), 50% (14-17min)
Flow rate: 0.8 ml/min
BPR 10 MPa
Temperature: 40°C
Detection: ESI-MS/MS, MRM
Sample 

(No.1)  Uridine
(No.2)  3’ ,5’ -Dibenzyloxyacetophenone
(No.3)  4-Ethylbenzenesulfonic Acid
(No.4)  Benzyltrimethylammonium

Sample conc. 8 µmol/l
Inj. Vol 2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Ordering Information

Particle Size: 2.5µm
Product Cat.No. PKG Size Price(US$)  
COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column (3.0 mm I.D. x 50 mm) NU05002 1 pkg N/A Inquire
COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column (3.0 mm I.D. x 100 mm) NU05005 1 pkg N/A Inquire
COSMOSIL 2.5HP (3-Hydroxyphenyl) Packed Column (3.0 mm I.D. x 150 mm) NU05006 1 pkg N/A Inquire
Particle Size: 3µm
Product Cat.No. PKG Size Price(US$)  
COSMOSIL 3HP (3-Hydroxyphenyl) Packed Column (2.0 mm I.D. x 150 mm) 13792-11 1 pkg 835.00 Buy
COSMOSIL 3HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 250 mm) 13793-01 1 pkg 885.00 Buy
Particle Size: 5µm
Product Cat.No. PKG Size Price(US$)  
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (2.0 mm I.D. x 150 mm) 13787-91 1 pkg 775.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 100 mm) 13971-61 1 pkg 600.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 150 mm) 13972-51 1 pkg 610.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (4.6 mm I.D. x 250 mm) 13788-81 1 pkg 775.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (10.0 mm I.D. x 150 mm) 13973-41 1 pkg 1,325.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (10.0 mm I.D. x 250 mm) 13789-71 1 pkg 1,765.00 Buy
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (20.0 mm I.D. x 150 mm) 13855-91 1 pkg N/A Inquire
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (20.0 mm I.D. x 250 mm) 13790-31 1 pkg N/A Inquire
COSMOSIL 5HP (3-Hydroxyphenyl) Packed Column (10.0 mm I.D. x 20 mm) 13791-21 1 pkg 660.00 Buy