COSMOGEL IEX Series
IEX
Ion Exchange Chromatography (IEX) has been widely used for analysis and purification of bio-molecules such as proteins, peptides and polynucleotides.
COSMOGEL IEX Series are available in 3 different ion exchange modes, Anion-exchange type, Cation-exchange type and Amphoteric ion-exchange type.
| Exchange Mode | Anion-exchange Type | Cation-exchange Type | Amphoteric ion-exchange Type | |||||
|---|---|---|---|---|---|---|---|---|
| Packing Material | Type Q | Type Q-N | Type S | Type S-N | Type M | Type M-N | ||
| Gel / Particle Size | Hydrophilic Polymer / 5µm | |||||||
| Pore Size | 1000 Å | Non-porous | 1000 Å | Non-porous | 1000 Å | Non-porous | ||
| Functional Group | -CH3N+(CH3)3 | -(CH2)3SO3- | -CH3N+(CH3)3 + -(CH2)3SO3- | |||||
| Binding Capacity | 110-150 mg | 12-20 mg | 70-100 mg | 10-18 mg | BSA: 55-75 mg/ml IgG: 35-50 mg/ml |
BSA: 6-10 mg/ml IgG: 5-9 mg/ml |
||
| BSA/ml-resin | human IgG/ml-resin | |||||||
| Target Sample | Acidic Proteins / DNA | Basic Proteins | All Proteins | |||||
| Application | Purification | Ultra-fast Analysis |
Precise Analysis |
Purification | Ultra-fast Analysis |
Precise Analysis |
Purification | Precise Analysis |
| Column Size | 4.6 x 50 | 4.6 x 30 | 4.6 x 100 | 4.6 x 50 | 4.6 x 30 | 4.6 x 100 | 4.6 x 50 | 4.6 x 100 |
Characteristic of Amphoteric ion-exchange
Amphoteric ion-exchange type (Type M) enables separation of both acidic and basic proteins in one sample at the same time.
| Column: | COSMOGEL IEX | Sample: | 1; Ribonuclease A 2; Cytochrome C 3; Trypsin Inhibitor 4; β-Galactosidase |
(1.5µg) (1.5µg) (6.0µg) (6.0µg) |
|
| Column size: | 4.6mmI.D.-50mm | ||||
| Mobile phase: | A: 20mmol/l Phosphate buffer(pH6.8) B: 20mmol/l Phosphate buffer(pH6.8) + 0.5mol/l NaCl B conc. 0→100% 30min Linear gradient |
||||
| Flow rate: | 0.5 ml/min | ||||
| Temperature: | 30°C | ||||
| Detection: | UV220nm |
Amphoteric ion-exchange type (Type M) can be used as Anion-exchange type or Cation-exchange type.
| Column: | COSMOGEL IEX | Sample: | 1. Carbonic Anhydrase 2. BSA |
(0.8µg) (6.3µg) |
|
| Column size: | 4.6mmI.D.-50mm | ||||
| Mobile phase: | A: 20mmol/l Tris buffer(pH8.3) B: 20mmol/l Tris buffer(pH8.3) + 0.5mol/l NaCl B conc. 0→100% 30min Linear gradient |
||||
| Flow rate: | 0.5 ml/min | ||||
| Temperature: | 30°C | ||||
| Detection: | UV220nm |
Large sample Purification
Type Q, Type S and Type M with porous packing materials have higher binding capacity of proteins than the respective non-porous type, which means that peak shape does not spread even with injection of a large volume of sample. Therefore they are highly suitable for purification of large sample.
| Column: | COSMOGEL IEX Type S | Sample: | 1. Ribonuclease A 2. Cytochrome C 3. Lysozyme |
|
| Column size: | 4.6mmI.D.-50mm | |||
| Mobile phase: | A: 20mmol/l Phosphate buffer(pH6.8) B: 20mmol/l Phosphate buffer(pH6.8) + 0.5mol/l NaCl B conc. 0→100% 30min Linear gradient |
|||
| Flow rate: | 0.5 ml/min | |||
| Temperature: | 30°C | |||
| Detection: | UV280nm |
Precise Analysis
Type Q-N, Type S-N, Type M-N with non-porous packing materials reduce spreading of samples in packing materials, result in high resolution separation for precise analysis such as quality control of antibody drugs. The longer column length also contributes to the sharper peaks.
| Column: | COSMOGEL IEX | Sample: | 1. Ribonuclease A 2. Cytochrome C 3. Lysozyme |
(1.65µg) (1.65µg) (1.65µg) |
|
| Mobile phase: | A: 20mmol/l Phosphate buffer(pH6.8) B: 20mmol/l Phosphate buffer(pH6.8) + 0.5mol/l NaCl B conc. 0→100% 30min Linear gradient |
||||
| Flow rate: | 0.5 ml/min | ||||
| Temperature: | 30°C | ||||
| Detection: | UV220nm |
Ultra-fast Analysis
The resolution with non-porous packing materials is not much affected by high flow rate and thus the materials are suitable for fast analysis. The shorter column length contributes to the fast analysis.
| Column: | COSMOGEL IEX | Sample: | 1. Ribonuclease A 2. Cytochrome C 3. Lysozyme |
(1.65µg) (1.65µg) (1.65µg) |
|
| Mobile phase: | A: 20mmol/l Phosphate buffer(pH6.8) B: 20mmol/l Phosphate buffer(pH6.8) + 0.5mol/l NaCl B conc. 0→100% Linear gradient Type S: 30min Type S-N: 10min |
||||
| Flow rate: | Type S: 0.5 ml/min, Type S-N: 1.0 ml/min |
||||
| Temperature: | 30°C | ||||
| Detection: | UV220nm |
Application Data
| Column: | COSMOGEL IEX Type M |
| Column size: | 4.6mmI.D.-50mm |
| Mobile phase: | A: 20mmol/l Tris buffer(pH8.3) B: 20mmol/l Tris buffer(pH8.3) + 0.5mol/l NaCl B conc. 0→100% 30min Linear gradient |
| Flow rate: | 0.5 ml/min |
| Temperature: | 30°C |
| Detection: | UV280nm |
| Sample: | Urease (100µg) |
| Column: | COSMOGEL IEX Type Q |
| Column size: | 4.6mmI.D.-50mm |
| Mobile phase: | A: 20mmol/l Tris buffer(pH8.3) B: 20mmol/l Tris buffer(pH8.3) + 0.5mol/l NaCl B conc. 0→100% 30min Linear gradient |
| Flow rate: | 0.5 ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
| Sample: | E.Coli Proteins |
| Column: | COSMOGEL IEX Type S |
| Column size: | 4.6mmI.D.-50mm |
| Mobile phase: | A: 20mmol/l Phosphate buffer(pH6.8) B: 20mmol/l Phosphate buffer(pH6.8) + 0.5mol/l NaCl B conc. 0→100% 30min Linear gradient |
| Flow rate: | 0.5 ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
| Sample: | Egg White |
| Column: | COSMOGEL IEX Type Q-N |
| Column size: | 4.6mmI.D.-30mm |
| Mobile phase: | A: 20mmol/l Tris buffer(pH8.3) B: 20mmol/l Tris buffer(pH8.3) + 0.5mol/l NaCl B conc. 10→60% 10min Linear gradient |
| Flow rate: | 1.0 ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
| Sample: | Anti-IgG(H+L), Mouse, Goat-Poly, Affinity purification |
| Column: | COSMOGEL IEX Type Q-N |
| Column size: | 4.6mmI.D.-100mm |
| Mobile phase: | A: 20mmol/l Tris buffer(pH8.3) B: 20mmol/l Tris buffer(pH8.3) + 0.5mol/l NaCl B conc. 10→20% 30min Linear gradient |
| Flow rate: | 0.5 ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
| Sample: | Anti-GFP(Mouse IgG1-κ), Monoclonal(GF200), AS (5.25µg) |
| Column: | COSMOGEL IEX Type S-N |
| Column size: | 4.6mmI.D.-100mm |
| Mobile phase: | A: 20mmol/l Acetate buffer(pH4.5) B: 20mmol/l Acetate buffer(pH4.5) + 0.5mol/l NaCl B conc. 30→40% 30min Linear gradient |
| Flow rate: | 0.5 ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
| Sample: | Anti-GFP(Mouse IgG1-κ), Monoclonal(GF200), AS (5.25µg) |
| Column: | COSMOGEL IEX Type Q-N |
| Column size: | 4.6mmI.D.-100mm |
| Mobile phase: | A: 20mmol/l Tris buffer(pH8.3) B: 20mmol/l Tris buffer(pH8.3) + 1.0mol/l NaCl B conc. 75→85% 30min Linear gradient |
| Flow rate: | 0.5 ml/min |
| Temperature: | 30°C |
| Detection: | UV260nm |
| Sample: | DNA (100b.p.Ladder) |
| Column: | COSMOGEL IEX Type Q-N |
| Column size: | 4.6mmI.D.-100mm |
| Mobile phase: | A: 20mmol/l Tris buffer(pH8.3) B: 20mmol/l Tris buffer(pH8.3) + 1.0mol/l NaCl B conc. 50→60% 30min Linear gradient |
| Flow rate: | 0.5 ml/min |
| Temperature: | 30°C |
| Detection: | UV260nm |
| Sample: | DNA (pd(A)40-60) |
Ordering Information
Amphoteric ion-exchange
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOGEL IEX Type M Packed Column, 4.6 mm I.D. x 50 mm | 06248-71 | Waters type | 1 pkg | 1,785.00 | Buy |
| COSMOGEL IEX Type M-N Packed Column, 4.6 mm I.D. x 100 mm | 06244-11 | Waters type | 1 pkg | 1,785.00 | Buy |
Anion-exchange
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOGEL IEX Type Q Packed Column4.6 mm I.D. x 50 mm | 06266-31 | Waters type | 1 pkg | 1,440.00 | Buy |
| COSMOGEL IEX Type Q-N Packed Column, 4.6 mm I.D. x 30 mm | 06264-51 | Waters type | 1 pkg | 1,315.00 | Buy |
| COSMOGEL IEX Type Q-N Packed Column, 4.6 mm I.D. x 100 mm | 06258-41 | Waters type | 1 pkg | 1,440.00 | Buy |
Cation-exchange
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOGEL IEX Type S Packed Column, 4.6 mm I.D. x 50 mm | 06252-01 | Waters type | 1 pkg | 1,440.00 | Buy |
| COSMOGEL IEX Type S-N Packed Column, 4.6 mm I.D. x 30 mm | 06251-11 | Waters type | 1 pkg | 1,315.00 | Buy |
| COSMOGEL IEX Type S-N Packed Column, 4.6 mm I.D. x 100 mm | 06250-21 | Waters type | 1 pkg | 1,440.00 | Buy |