COSMOSIL Diol-II: Gel Filtration Chromatography
Gel Filtration Chromatography
COSMOSIL Diol-120-II and Diol-300-II gel filtration columns are ideal for the size-based separation of proteins and other water soluble polymers. The separation MW range is 5,000-700,000 daltons for proteins and 300-300,000 daltons for water soluble polymers when Diol-120-II and Diol-300-II are used in series.
Features
- Ideal for the size-based separation of proteins and water soluble polymers
- Reduce undesirable adsorption
| Packing Material | 5Diol-120-II | 5Diol-300-II |
|---|---|---|
| Silica Gel | High Purity Porous Spherical Silica | |
| Average Particle Size | 5 µm | |
| Average Pore Size | approx. 120Å | approx. 300Å |
| Stationary Phase | Diol Group | |
| Object Substance | Protein and Water Soluble Polymer | |
| Flow Rate | 0.5-1.0 ml/min | |
| Selection of Pore Size (Proteins) | 5,000-100,000 | 10,000-700,000 |
| Selection of Pore Size (Water Soluble Polymer) | 300-30,000 | 500-300,000 |
Calibration Curve of Proteins
Condition
| Column: | COSMOSIL 5Diol-II 7.5mmI.D. x 600mm |
| Mobile phase: | 20mmol/l Phosphate Buffer (pH 7.0) +100mmol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30 °C |
| Sample | MW | Sample | MW | |
|---|---|---|---|---|
| Thyroglobulin | 660,000 | Peroxidase | 40,000 | |
| Catalase | 250,000 | Carbonic Anhydrase | 30,000 | |
| Glucose Oxidase | 160,000 | α -Chymotrypsinogen A | 25,700 | |
| Uricase | 128,000 | α -Chymotrypsin | 25,200 | |
| Choline Oxidase | 95,000 | Trypsinogen | 24,000 | |
| Transferrin | 85,000 | Trypsin (bovine) | 23,300 | |
| Conalbumin | 77,500 | Myoglobin | 17,000 | |
| Malate Dehydrogenase | 70,000 | Lysozyme | 14,300 | |
| α -Glucosidase | 68,500 | Ribonuclease A | 13,700 | |
| Albumin (BSA) | 66,000 | Cytochrome C | 12,400 | |
| α -Amylase | 52,500 | Aprotinin | 6,500 | |
| Fetuin | 48,000 | Gly-Gly | 132 | |
| Albumin (Ovalbumin) | 45,000 |
Comparison with Competitors' Columns
COSMOSIL Diol-II series shows excellent separation compared to competitors' columns.
| Column |
Column Size | Gel | Particle Size (avg.) | Pore Size (avg.) | Chart Speed |
|---|---|---|---|---|---|
| COSMOSIL 5Diol-300-II | 7.5 mm I.D. x 300 mm |
Silica Gel | 5 µm | approx. 300Å | 5 mm/min |
| TSKgel G3000 SWXL | 7.8 mm I.D. x 300 mm |
Silica Gel | 5 µm | 250Å | 5 mm/min |
| COSMOSIL 5Diol-120-II | 7.5 mm I.D. x 300 mm |
Silica Gel | 5 µm | approx. 120Å | 5 mm/min |
| TSKgel G2000 SWXL | 7.8 mm I.D. x 300 mm |
Silica Gel | 5 µm | 125Å | 5 mm/min |
COSMOSIL 5Diol-300-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Ferritin (Horse)2. Peroxidase (Horse)
3. Cytochrome C (Horse)
4. Phenylglycine
COSMOSIL 5Diol-300-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Transferrin (Human)2. Hemoglobin (Human)
3. Angiotensin II (Human)
COSMOSIL 5Diol-120-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Sarcosine Dehydrogenase2. Lysozyme
3. Phenylglycine
COSMOSIL 5Diol-120-II
Condition
| Mobile phase: | 0.01mol/l Na2HPO4+0.01mol/l NaH2PO4+0.1mol/l Na2SO4 |
| Flow rate: | 1.0ml/min |
| Temperature: | 30°C |
| Detection: | UV220nm |
Sample
1. Sarcosine Dehydrogenase2. Lysozyme
3. Phenylglycine
Application Data
E. Coli Proteins
Sodium Polystyrene Sulfonate
Polyethylene Glycols
Pullulan
Human Serum
Ordering Information
COSMOSIL 5Diol-120-II
| Product | Cat.No. | End-Fitting | Quantity | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL 5Diol-120-II Packed Column, 7.5 mm I.D. x 300 mm | 38050-51 | Waters type | 1 pkg | 1,050.00 | Buy |
| COSMOSIL 5Diol-120-II Packed Column, 7.5 mm I.D. x 600 mm | 38051-41 | Waters type | 1 pkg | 1,585.00 | Buy |
| COSMOSIL 5Diol-120-II Guard Cartridge, 4.6 mm I.D. x 10 mm, 2 pieces | 19180-54 | Waters type | 1 pkg | 220.00 | Buy |
| COSMOSIL 5Diol-120-II Guard Column, 7.5 mm I.D. x 50 mm | 38049-91 | Waters type | 1 pkg | 495.00 | Buy |
COSMOSIL 5Diol-300-II
| Product | Cat.No. | End-Fitting | Quantity | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL 5Diol-300-II Packed Column, 7.5 mm I.D. x 300 mm | 38053-21 | Waters type | 1 pkg | 1,050.00 | Buy |
| COSMOSIL 5Diol-300-II Packed Column, 7.5 mm I.D. x 600 mm | 38054-11 | Waters type | 1 pkg | 1,585.00 | Buy |
| COSMOSIL 5Diol-300-II Guard Cartridge, 4.6 mm I.D. x 10 mm, 2 pieces | 19179-94 | Waters type | 1 pkg | 220.00 | Buy |
| COSMOSIL 5Diol-300-II Guard Column, 7.5 mm I.D. x 50 mm | 38052-31 | Waters type | 1 pkg | 495.00 | Buy |
COSMOSIL Guard Cartridge Holder
| Product | Cat.No. | End-Fitting | Quantity | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL Guard Cartridge Holder, 4.6 mm I.D. | 38009-79 | Waters type | 1 pkg | 275.00 | Buy |