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Protein Ladder | Protein Research

Protein Research

Protein Ladder

Prestained Protein Ladder, Broad Range

The Prestained Protein Ladder, Broad Range is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine builer). The Prestained Protein Ladder, Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

  • 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
  • 1.5~2.5 µl per well for general Western transferring.
  • Apply more for thicker (> 1.5 mm) or larger gel.
Tris-Glycine 4-20%, Bis-Tris 10% BOPS buffer, Bis-Tris 10% MES buffer

Contents
Approximately 0.1-0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Quality Control
Under suggested conditions, Prestained Protein Ladder, Broad Range resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.

Storage
Stable for up to 2 weeks at 25°C
Stable for up to 3 months at 4°C
For long term storage, store at -20°C

Ordering information

Product Storage Cat.No. Quantity Price(US$)  
Prestained Protein Ladder, Broad Range -20°C NU01002 500 µl 100 Buy Now

Protein Ladder

Pre-stained Protein Markers for SDS-PAGE

Easy identification :
alternate blue and violet stained bands
Great lot-to-lot consistency :
for reliable comparison

Features

  • Only small amount to use: high concentrarion of prestained proteins. (2.5 - 5.0µl/well)
  • Marker proteins are visible during electrophoresis.
  • Prestained bands remain visible after the gel is stained (CBB, silver, negative)
  • Color remains the same even after blotting

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Application

CBB Staining
The bands of the commercially available protein markers can be seen only after CBB staining, while the Prestained Protein Markers show clear bands before and after CBB staining.

SDS-PAGE (5-20% gel)
1,5. commercially available 
          protein markers
2. Prestained Protein Markers 
      (Low range)
3. Prestained Protein Markers 
      (High range)
4. Prestained Protein Markers 
      (Broad range)

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Western Blotting
The bands of proteins from the Prestained Protein Markers are irreversibly bound to the hydrophobic membrane after blotting. The two colors remain distinguishable.

PVDF membrane
1.  Prestained Protein Markers 
      (Low range)
2.  Prestained Protein Markers 
      (High range)
3.  Prestained Protein Markers 
      (Broad range)

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Ordering information

Product Storage Cat.No. Quantity Price(US$)  
Pre-stained Protein Markers (Broad Range) for SDS-PAGE -20°C 02525-35 500 µl 79 Buy Now
Pre-stained Protein Markers (High Range) for SDS-PAGE -20°C 26039-75 500 µl 79 Buy Now
Pre-stained Protein Markers (Low Range) for SDS-PAGE -20°C 28941-75 500 µl 79 Buy Now
Pre-stained Protein Markers (Low Range) for SDS-PAGE -20°C 28944-74 5 x 100 µl 85 Buy Now

Protein Ladder

Unstained Protein Moleculer Weight Markers (10x) for SDS-PAGE

Description

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Ordering information

Product Storage Cat.No. Quantity Price(US$)  
Protein Markers (MW6,500-200,000) (10X) for SDS-PAGE -20°C 29458-24 200 µl 59 Buy Now
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