Nacalai USA, Inc., a wholly-owned subsidiary of Nacalai Tesque, Inc., Kyoto Japan., was founded in January 2005 in San Diego, California to bridge between the United States and Japan in the scientific research community. Nacalai USA strives to bring the most innovative and stringently quality-controlled biochemicals and research tools from Japan into the United States market, and also to introduce United States technologies and products into the Japanese research market.
Nacalai USA provides the highest quality research products necessary for the Life Science research such as Molecular Biology and Proteomics. Most of the products are manufactured and packaged in the high level of manufacturing facilities in Japan.
Nacalai USA also provides Cosmosil brand HPLC columns. The Cosmosil chromatography product line includes several robust reversed phase and prep columns as well as specialty columns such as the Cholester (cholesteryl group), NPE (nitrophenylethyl group), PYE (pyrenylethyl group) and Sugar-D. Cosmosil Buckyprep (pyrenylpropyl group) and PBB (pentabromobenzyl group) columns are well recognized worldwide for Fullerene separation.
Biochemical
Substrates
- N-Acetyl-D-mannosamine, Monohydrate [ManNAc] Prepared by enzymatic hydrolysis of N-acetylneuraminic acid, and purified by crystallization
- N-Acetylneuraminic acid (Enzymation) [NANA ; Sialic acid ; NeuAc]
- N-Acetylneuraminic acid, dimer(α,2 → 8) [DP2]
- N-Acetylneuraminic acid, trimer(α,2 → 8) [DP3]
- N-Acetylneuraminic acid, tetramer(α,2 → 8) [DP4]
- N-Acetylneuraminic acid, pentamer(α,2 → 8) [DP5]
- N-Acetylneuraminic acid, hexamer(α,2 → 8) [DP6]
- N-Acetylneuraminic acid, oligomer(α,2 → 8) kit
- N-Acetylneuraminyl-α (2 → 6)-lactose [6' -Sialyllactose]
- Colominic acid, Sodium salt
- Cytidine-5'-monophospho-N-acetylneuraminic acid, Disodium salt [CMP -Neu5Ac·2NA]
- 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid [NeuAc2en ; NeuNAc2en ; Neu5Ac2en]
- N-Glycolylneuraminic acid [NeuGc ; Neu5Gc ; NeuNGc]
- 4-Methylumbelliferyl-N-acetyl-α-D-neuraminic acid, Ammonium salt [4-MU-NANA]
- Uridine-5'-diphosphoglucose, Disodium salt
- Uridine-5'-diphosphoglucuronic acid, Trisodium salt
Enzymes
- 3α-Hydroxysteroid dehydrogenase 3α-Hydroxysteroid : NAD(P)+ oxidoreductase [EC 1.1.1.50]
- β-Hydroxysteroid dehydrogenase 3(or17) β-Hydroxysteroid : NAD(P)+ oxidoreductase [EC 1.1.1.51]
- NADH oxidase
- N-Acetylneuraminic acid aldolase N-Acetylneuraminate pyruvate lyase [EC 4.1.3.3]
- Acylglucosamine 2-epimerase (Renin binding protein) Acylglucosamine 2-epimerase [EC 5.1.3.8]
- Neuraminidase [Sialidase] Acylneuraminyl hydrolase [EC 3.2.1.18]
- Neuraminidase isozyme S [Reagent for studies of gangliosides]
Green tea
- (-)-Epicatechin [(-)-EC]
- (-)-Epigallocatechin [(-)-EGC]
- (-)-Epicatechin gallate [(-)-ECg]
- (-)-Epigallocatechin gallate [(-)-EGCg]
- (+)-Catechin Hydrate [(+)-C]
- (-)-Catechin [(-)-C]
- (-)-Gallocatechin [(-)-GC]
- (-)-Catechin gallate [(-)-Cg]
- (-)-Gallocatechin gallate [(-)-GCg]
- (+)-Epicatechin [(+)-EC]
- (-)-Epicatechin-3'-O-methylether [(-)-EC-3'-O-Me]
- (-)-Epicatechin-4'-O-methylether [(-)-EC-4'-O-Me]
- (-)-Epigallocatechin-3'-O-methylether [(-)-EGC-3'-O-Me]
- (-)-Epigallocatechin-4'-O-methylether [(-)-EGC-4'-O-Me]
- (-)-Epicatechin-3'-O-methylether gallate [(-)-ECg-3'-O-Me]
- (-)-Epicatechin-4'-O-methylether gallate [(-)-ECg-4'-O-Me]
- (-)-Epicatechin 3-(3''-O-methyl)gallate [(-)-ECg-3''-O-Me]
- (-)-Epicatechin 3-(4''-O-methyl)gallate [(-)-ECg-4''-O-Me]
- (-)-Epigallocatechin-3'-O-methylether Gallate [(-)-EGCg-3'-O-Me]
- (-)-Epigallocatechin-4'-O-methylether Gallate [(-)-EGCg-4'-O-Me]
- (-)-Epigallocatechin 3-(3"-O-methyl)gallate [(-)-EGCg-3"-O-Me]
- (-)-Epigallocatechin 3-(4"-O-methyl)gallate
- Catechin Mixture {(-)-Epicatechin, (-)-Epigallocatechin, (-)-Epicatechin gallate, (-)-Epigallocathechin gallate}, from Green tea
- Strictinin
Black tea
Soy bean
- Daidzein
- Glycitein
- Genistein
- Daidzin
- Glycitin
- Genistin
- 6"-O-Acetyldaidzin
- 6"-O-Acetylglycitin
- 6"-O-Acetylgenistin
- 6"-O-Malonyldaidzin
- 6"-O-Malonylglycitin
- 6"-O-Malonylgenistin
- Isoflavone Aglycon Mixture A
- Isoflavone Aglycon Mixture B
- Isoflavone Glucoside Mixture A
- Isoflavone Glucoside Mixture B
Others
- Curcumin 1
- Curcumin 2
- Curcumin 3
- Ginkgolide A
- Ginkgolide B
- Ginkgolide C
- (-)-Bilobalide
- Ginkgolic Acid 15:1 [Ginkgolic Acid, Ginkgolic Acid I]
- Ginkgolic Acid 17:1 [Ginkgolic Acid II]
- Ginkgolic Acid 13:0
- Ginkgolic Acid 15:0
- Ginkgolic Acid 15:1 + 13:0(4:1)
- Ginkgolic Acid 17:1 + 15:0(6:1)
- Shikonin
- Shikonin(Shikonin/Alkannin≒6:1)
- Shikalkin(Shikonin/Alkannin≒1:1)
- Alkannin
- Acetylshikonin
- Isobutyrylshikonin
- β-Hydroxyisovalerylshikonin
- Isovalerylshikonin
- α-Methyl-n-butyrylshikonin
- β,β-Dimethylacrylshikonin
- (E)-10-Hydroxy-2-decenoic Acid
- Glycyrrhizin(Glycyrrhizic acid)
- Glycyrrhizic Acid Monoammonium Salt n-Hydrate
- Glycyrrhizic Acid Dipotassium Salt
- 18β-Glycyrrhetinic Acid(18β-Glycyrrhetic acid)
- Glycyrrhetic Acid 3-O-Glucuronide
- Schizandrin
- Gomisin A
- Gomisin N
- Deoxyschizandrin
- (±)-γ-Schizandrin
- Wuweizisu C
- Capsaicin
- Dihydrocapsaicin
- Nordihydrocapsaicin
- N-Vanillylnonanamide
- N-Vanillyldecanamide
- Clove 3
- Biflorin
- Casuarictin
- Eugeniin
- Tellimagrandin I
- 1,3-Di-O-galloyl-4,6-O-(S)- hexahydroxydiphenoyl-beta-D-glucose
- Rhynchophylline
Cell Culture
Cosmosil HPLC Columns
COSMOSIL C18-MS-II: Monomeric C18
COSMOSIL 5C18-MS-II is a monomeric type of C18 phase.
- COSMOSIL 5C18-MS-II Guard Column
- COSMOSIL 5C18-MS-II Packed Column
- COSMOSIL 2.5C18-MS-II Packed Column
- COSMOSIL Guard Cartridge Holder
COSMOSIL C18-AR-II: Polymeric C18
COSMOSIL 5C18-AR-II is a polymeric type of C18 phase.
COSMOSIL C18-PAQ: For aqueous conditions
COSMOSIL 5C18-PAQ is designed to offer superior retention of polar compounds and excellent reproducibility in highly aqueous mobile phases, even in 100% aqueous.
Other reversed phases
COSMOSIL Buckyperp: Preparative separation of Fullerenes
COSMOSIL Buckyprep is a pyrenylpropyl group bonded silica based column specifically designed for fullerene separation. The unparalleled separation capabilities have enabled COSMOSIL Buckyprep to become the world benchmark of HPLC column for fullerene separation. COSMOSIL Buckyprep retains fullerenes very strongly with a mobile phase of 100% toluene and exceeds the injection volume of a standard C18 column by a factor of 35. Therefore, preparative-scale separation can be obtained with a 250 mm x 4.6 mm I.D. analytical column.
COSMOSIL Buckyprep-M: For Metallofullerenes
COSMOSIL Buckyprep-M is a phenothiazinyl group bonded silica based column specifically designed for metallo-fullerene separation. Metallo-fullerenes are retained more strongly than other fullerenes on this column. COSMOSIL Buckyprep-M is also effective for the separation of higher fullerenes and fullerene derivatives.
COSMOSIL PBB: Preparative separation of Fullerenes and higher Fullerenes.
COSMOSIL PBB is a pentabromobenzyl group bonded silica based column useful for preparative-scale separation of fullerenes. It can be used with carbon disulfide or 1,2,4-trichlorobenzene, which has greater solubility for fullerenes than toluene. The loading capacity of COSMOSIL PBB for C60 and C70 can be three times greater than COSMOSIL Buckyprep
COSMOSIL PYE, COSMOSIL NPE
COSMOSIL PYE (pyrenylethyl group bonded) and COSMOSIL NPE (nitrophenylethyl group bonded) column show unique retention characteristics based on multiple separation modes such as hydrophobic, charge transfer and π-π interactions. These columns are recommended for the separation of structural isomers.
- COSMOSIL 5PYE Guard Column
- COSMOSIL 5PYE Packed Column
- COSMOSIL 5NPE Guard Column
- COSMOSIL 5NPE Packed Column
COSMOSIL Cholester: Hydrophobic interaction and planarity selectivity
COSMOSIL Cholester is a new silica based RP column which employs a cholestery bonded phase. Cholester provides enhanced selectivity over traditional C18 materials and greater performance in separating isomers or other closely related compounds. Cholester is ideal for method development and serves as an excellent alternative to traditional C18 columns.
- COSMOSIL Cholester Guard Column
- COSMOSIL Cholester Packed Column
- COSMOSIL 2.5 Cholester Packed Column
COSMOSIL Sugar-D: Novel stationary phase for saccharide separation
Conventionally Aminopropyl bounded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional Aminopropyl bounded phases are tailing and adsorption of certain saccharides and general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL Sugar-D, resulting in better (sharper) separation and much improved durability. In addition COSMOSIL Sugar-D is useful in the separation of highly hydrophilic compounds which are not retained in conventional octadecyl (ODS) bounded stationary phases.
COSMOSIL Diol-II: Aqueous gel filtration chromatography
COSMOSIL SL-II: Normal phase chromatography
COSMOSIL C18-OPN: Reversed Phase Open Column Chromatography
Open column chromatography is an excellent and easy technique for large scale preparation and purification at low cost. Reversed phase C18 packing materials are used but restricted to about 30-50% water in the mobile phase. The COSMOSIL C18-OPN is a new "Water-Wet" C18 packing material developed for Reversed Phase Open Column Chromatography. The C18-OPN material can be used in 100% aqueous eluents.
COSMOSIL C18-PREP: Process scale purifications
COSMOSIL HILIC: Hydrophilic interaction
COSMOSIL HILIC is a new column for hydrophilic interaction chromatography with Triazole bonded silica packing material. The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called "aqueous normal phase", the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase, thus acidic compound is strongly retained.
Molecular Biology
Protein Research
CosmoPAGE Pre-cast gels
The CosmoPAGE pre-cast gel is ideal for protein electrophoresis. The unique formulation of polyacrylamide provides the best resolution, most strength, best transfer efficiency, and longest shelf life. CosmoPAGE pre-cast gels are available in two product lines: SDS Gel and Native Gel. Each product line has gels available in 1-well, 12-well and 17-well formats for high-throughput protein electrophoresis. CosmoPAGE SDS Gel is also available for 2D electrophoresis. CosmoPAGEM Native Gel is suitable for nucleic acid electrophoresis as well.
- CosmoPAGE SDS Gel 8% 12 well
- CosmoPAGE SDS Gel 8% 17 well
- CosmoPAGE SDS Gel 10% 1 well
- CosmoPAGE SDS Gel 10% 12 well
- CosmoPAGE SDS Gel 10% 17 well
- CosmoPAGE SDS Gel 12% 1 well
- CosmoPAGE SDS Gel 12% 12 well
- CosmoPAGE SDS Gel 12% 17 well
- CosmoPAGE SDS Gel 16% 12 well
- CosmoPAGE SDS Gel 16% 17 well
- CosmoPAGE SDS Gel 20% 12 well
- CosmoPAGE SDS Gel 20% 17 well
- CosmoPAGE SDS Gel 4-8% 12 well
- CosmoPAGE SDS Gel 4-8% 17 well
- CosmoPAGE SDS Gel 4-12% 12 well
- CosmoPAGE SDS Gel 4-12% 17 well
- CosmoPAGE SDS Gel 4-20% 2D well
- CosmoPAGE SDS Gel 4-20% 12 well
- CosmoPAGE SDS Gel 4-20% 17 well
- CosmoPAGE SDS Gel 10-20% 12 well
- CosmoPAGE SDS Gel 10-20% 17 well
- CosmoPAGE Native Gel 10% 12 well
- CosmoPAGE Native Gel 10% 17 well
- CosmoPAGE Native Gel 20% 12 well
- CosmoPAGE Native Gel 20% 17 well
- CosmoPAGE Native Gel 2-8% 12 well
- CosmoPAGE Native Gel 2-8% 17 well
- CosmoPAGE Native Gel 3-20% 12 well
- CosmoPAGE Native Gel 3-20% 17 well
- LDS Sample Buffer (4x)
- DTT Reducer (10x)
- SDS-Std. Run Buffer (20x)
- SDS-Low Range Run Buffer (20x) *not recommended for "highly concentrated gel"
- Native Sample Buffer (4x)
- Native Run Buffer (20x)
- TGS Transfer Buffer (10x/20x)
- Tris(hydroxymethyl)aminomethane
- Tricine {N-[Tris(hydroxymethyl)methyl]glycine}
- Glycine
- Sodium Lauryl Sulfate (SDS)
- Dithiothreitol
- CosmoPAGE SDS Gel 4-20% 12 well (8 cm x 10 cm)
- CosmoPAGE SDS Gel 12 % 12 well (8 cm x 10 cm)
- InstantBlue
- Rapid Stain CBB Kit (for 2L)
- Antioxidant 800x
CosmoPAGE Dual Run and Blot System
- Dual Run & Blot System
- Tank Assembly, Dual Run & Blot
- Lid Assenbly, Dual Run & Blot
- Core Assembly, Dual Run & Blot
- Blotting Cassette w/pad 9x9 cm
- Cooling Block, Dual Run & Blot
- Buffer Dam, Dual Run & Blot
- Sponge Pad Kit, 9 x9 cm
- Blot Sandwich NC 90x85 mm
- Blot Sandwich PVDF 90x85 mm
- CosmoPAGE Gel Drying Solution (1x)
Molecular Weight Markers
- Pre-stained Protein Markers (Broad Range) for SDS-PAGE
- Pre-stained Protein Markers (High Range) for SDS-PAGE
- Pre-stained Protein Markers (Low Range) for SDS-PAGE
- Protein Markers (MW6,500-200,000) (10X) for SDS-PAGE
Rapid Stain CBB Kit
CBB Stain One (Ready to Use)
Gel-Negative Stain Kit
Gel-Negative Stain Kit can stain proteins in SDS-PAGE in 20 minutes. The sensitivity is similar to silver staining. Since Gel-Negative Stain binds to the gel but not the proteins, the proteins are not altered and, therefore, better for downstream application.
Sil-Best stain One
The Sil-Best Stain One provides maximal silver staining sensitivity with minimal protein modification for optimal mass spectrometry results. The mass spectrometry problems associated with traditional silver stains are eliminated with the Sil-Best Stain One because the reagents used do not modify protein side chains and do not interfere with trypsin digestion. In 80 minutes, 0.4 ng of protein can be detected with low background.
Other staining reagents
- Coomassie Brilliant Blue G-250
- Coomassie Brilliant Blue R-250
- Dispotray (for minigel staining)
- Amido Black 10B
- Ponceau S
Blocking Solution for immunoassays
Blocking is indispensable in immunoassays in order to inhibit non-specific binding reactions. Blocking One contains both high molecular weight compounds and bovine serum protein. The result is superior compared to conventional blocking solutions. The preservative does not affect the enzyme activity of peroxidase (POD) or alkaline phosphatase (ALP). Only simple refrigerator storage is necessary, even after opening the bottle.
Other blocking reagent
WB Stripping Solution
WB Stripping Solution removes conjugated antibodies from blots, enabling subsequent detections with different antibodis on the very same blot. After the first antigen-antibody reaction and following chemiluminescent visualization, the antibody can be removed by the WB Stripping Solution. A second antigen-antibody reaction can be conducted on the same blot. The same blot can be probed 2-5 times if chemiluminescent detection is employed. Alternatively, one blot can be used multiple times under different conditions to optimise the result of antigen-antibody reaction.
Protease Inhibitor Cocktail (EDTA free)
Inhibition of intra and extra cellular proteases is vital to purify and collect the expressed proteins. Saving trouble of finding adequate inhibitors, a wide range of proteases are inhibited by the Protease Inhibitor Cocktail. This product does not interfere with metalloproteins since it is EDTA free.
- Protease Inhibitor Cocktail (EDTA free) (100X) Lyophilized Powder
- Protease Inhibitor Cocktail for General Use (100X) Lyophilized Powder
- Protease Inhibitor Cocktail for Use with Mammalian Cell and Tissue Extracts (100x) DMSO Solution
Phosphatase Inhibitor Cocktail
Phosphatase Inhibitor Cocktail is a mixture of several inhibitors to protect valuable proteins from dephosphorylation. The product preserves phosphorylated proteins existing in small quantity in cells and tissues
Epitope Tag Antibody
