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Gel Filtration Chromatography (COSMOSIL Diol-II)

COSMOSIL Diol-120-II, Diol-300-II and Diol-1000-II gel filtration columns are ideal for the size-based separation of proteins and other water soluble polymers. The separation MW range is 5,000-700,000 daltons for proteins and 1000-500,000 daltons for water soluble polymers when Diol-120-II, Diol-300-II and Diol-1000-II are used in series.

Features

  • Ideal for the size-based separation of proteins and water soluble polymers
  • Reduce undesirable adsorption
Packing Material5Diol-120-II5Diol-300-II5Diol-1000-II5Diol-2000-II
USP code L20
Silica Gel High purity porous spherical silica *
Average Particle Size 5 µm
Average Pore Size approx. 120Å approx. 300Å approx. 1000Å approx. 2000Å
Stationary Phase Diol group
Suitable Samples Protein, Water-Soluble Polymer
Flow Rate 0.5-1.0 ml/min
Selection of Pore Size (Proteins) 5,000-100,000 10,000-700,000 - -
Selection of Pore Size (Water Soluble Polymer) 1,000-20,000 5,000-100,000 50,000-500,000 -

* With the silica-based gel, organic solvents, including methanol and acetonitrile, can be use.

Calibration Curve of Proteins

Condition

Column: COSMOSIL 5Diol-II 7.5mmI.D. x 600mm
Mobile phase: 20mmol/l Phosphate Buffer (pH 7.0)
+100mmol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30 °C
Sample MW   Sample MW
Thyroglobulin 660,000   Peroxidase 40,000
Catalase 250,000   Carbonic Anhydrase 30,000
Glucose Oxidase 160,000   α -Chymotrypsinogen A 25,700
Uricase 128,000   α -Chymotrypsin 25,200
Choline Oxidase 95,000   Trypsinogen 24,000
Transferrin 85,000   Trypsin (bovine) 23,300
Conalbumin 77,500   Myoglobin 17,000
Malate Dehydrogenase 70,000   Lysozyme 14,300
α -Glucosidase 68,500   Ribonuclease A 13,700
Albumin (BSA) 66,000   Cytochrome C 12,400
α -Amylase 52,500   Aprotinin 6,500
Fetuin 48,000   Gly-Gly 132
Albumin (Ovalbumin) 45,000      

Comparison with Competitors' Columns

COSMOSIL Diol-II series shows excellent separation compared to competitors' columns.

Column

Column Size

GelParticle
Size (avg.)
Pore Size
(avg.)
Chart
Speed
COSMOSIL 5Diol-300-II 7.5 mm I.D.
x 300 mm
Silica Gel 5 µm approx. 300Å 5 mm/min
TSKgel G3000 SWXL 7.8 mm I.D.
x 300 mm
Silica Gel 5 µm 250Å 5 mm/min
COSMOSIL 5Diol-120-II 7.5 mm I.D.
x 300 mm
Silica Gel 5 µm approx. 120Å 5 mm/min
TSKgel G2000 SWXL 7.8 mm I.D.
x 300 mm
Silica Gel 5 µm 125Å 5 mm/min

COSMOSIL 5Diol-300-II

COSMOSIL 5Diol-300-II, TSKgel G3000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Ferritin (Horse)
2. Peroxidase (Horse)
3. Cytochrome C (Horse)
4. Phenylglycine
 

COSMOSIL 5Diol-300-II

COSMOSIL 5Diol-300-II, TSKgel G3000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Transferrin (Human)
2. Hemoglobin (Human)
3. Angiotensin II (Human)
 

COSMOSIL 5Diol-120-II

COSMOSIL5Diol-120-II, TSKgel G2000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Sarcosine Dehydrogenase
2. Lysozyme
3. Phenylglycine
 

COSMOSIL 5Diol-120-II

COSMOSIL5Diol-120-II, TSKgel G2000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Sarcosine Dehydrogenase
2. Lysozyme
3. Phenylglycine
 

Applications

E. Coli Proteins

COSMOSIL Application Data

Sodium Polystyrene Sulfonate

COSMOSIL Application Data

Polyethylene Glycols

COSMOSIL Application Data

Pullulan

COSMOSIL Application Data

Human Serum

COSMOSIL Application Data

Application: Polystyrene

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 5Diol-120-II Packed Column (4.6 mm I.D. x 150 mm) 07490-51 Waters type 1 pkg 642.00 Buy
COSMOSIL 5Diol-120-II Packed Column (4.6 mm I.D. x 250 mm) 06888-91 Waters type 1 pkg 803.00 Buy
COSMOSIL 5Diol-120-II Packed Column (7.5 mm I.D. x 300 mm) 38050-51 Waters type 1 pkg 1,124.00 Buy
COSMOSIL 5Diol-120-II Packed Column (7.5 mm I.D. x 600 mm) 38051-41 Waters type 1 pkg 1,696.00 Buy
COSMOSIL 5Diol-120-II Packed Column (10.0 mm I.D. x 250 mm) 12282-21 Waters type 1 pkg - Inquire
COSMOSIL 5Diol-300-II Packed Column (4.6 mm I.D. x 150 mm) 07223-51 Waters type 1 pkg 642.00 Buy
COSMOSIL 5Diol-300-II Packed Column (4.6 mm I.D. x 250 mm) 13086-71 Waters type 1 pkg 803.00 Buy
COSMOSIL 5Diol-300-II Packed Column (7.5 mm I.D. x 300 mm) 38053-21 Waters type 1 pkg 1,124.00 Buy
COSMOSIL 5Diol-300-II Packed Column (7.5 mm I.D. x 600 mm) 38054-11 Waters type 1 pkg 1,696.00 Buy
COSMOSIL 5Diol-1000-II Packed Column (7.5 mm I.D. x 300 mm) 13338-71 Waters type 1 pkg 2,140.00 Buy
COSMOSIL 5Diol-2000-II Packed Column (7.5 mm I.D. x 300 mm) 19976-31 Waters type 1 pkg 2,140.00 Buy
Guard Column / Guard Cartridge
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 5Diol-120-II Guard Column (7.5 mm I.D. x 50 mm) 38049-91 Waters type 1 pkg 530.00 Buy
COSMOSIL 5Diol-300-II Guard Cartridge (4.6 mm I.D. x 10 mm) 19180-54 Waters type 2 pkg 236.00 Buy
COSMOSIL 5Diol-300-II Guard Column (7.5 mm I.D. x 50 mm) 38052-31 Waters type 1 pkg 530.00 Buy
COSMOSIL 5Diol-1000-II Guard Column (7.5 mm I.D. x 50 mm) 13337-81 Waters type 1 pkg 535.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** 38009-79 Waters type 1 pkg - Inquire
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy

#38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).