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Anti-DYKDDDDK (Mouse) Monoclonal

DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. Anti-DYKDDDDK (Mouse IgG2b-κ) is a high-affinity monoclonal antibody that can binds to same epitope as Sigma's Anti-FLAG® M2 antibody.

Anti-DYKDDDDK (Mouse IgG2b-κ), Monoclonal (5A8E5)

Clone : 5A8E5
Isotype : IgG2b-κ (Mouse)
Product form : Lyophilized form
Specificity : Anti-DYKDDDDK (Mouse IgG2b-κ) recognizes C-terminal, N-terminal and internal tagged fusion proteins.
Concentration : 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Immunogen : A synthetic peptide (DYKDDDDK) coupled KLH
Application : Western Blotting 0.1-1.0 µg/ml
Immunoprecipitation 1 µg/ml
Immunofluorescent 1 µg/ml
ELISA 0.05-0.2 µg/ml

Anti-DYKDDDDK (Mouse), Monoclonal (6H8E4), Agarose Conjugate

Clone : 6H8E4
Degree of substitution : Approx. 8.5 mg Anti-DYKDDDDK mAb / ml settled resin
Product form : 1x PBS containing 50% glycerol

Comparison of Anti-DYKDDDDK (Mouse IgG2b-κ) (#NU01102) with Mouse Anti-DYKDDDDK Tag mAb (Company S, clone M2, 1 µg/ml) by Western blot

Sample : Lane 1. N-terminal DYKDDDDK-tagged fusion protein
Lane 2. Internal DYKDDDDK-tagged fusion protein
Lane 3. C-terminal DYKDDDDK-tagged fusion protein
Primary antibody : (A) Anti-DYKDDDDK (Mouse IgG2b-κ), Monoclonal (5A8E5), 1 mg/ml
(B) Company S, Anti-DYKDDDDK Tag mAb (M2), 1 mg/ml
Secondary antibody : Goat anti-Mouse IgG IRDye™ 800 Conjugated

Sample : Lane 1-3 Multiple Tag (Purified) (400 ng, 80 ng,16 ng)
Lane 4. 293 cell lysate 10 m
Primary antibody : (A) Anti-DYKDDDDK (Mouse IgG2b-κ), Monoclonal (5A8E5), 1 mg/ml
(B) Company S, Anti-DYKDDDDK Tag mAb (M2), 1 mg/ml
Secondary antibody : Goat anti-Mouse IgG IRDye™ 800 Conjugated

Consistent analysis of Batch 1# and Batch 2# of Anti-DYKDDDDK (Mouse IgG2b-κ) by Western Blotting

The figure shows that signal remains consistent from Lot to Lot.
The assay was performed with DYKDDDDK-tagged fusion protein.
The signal was developed with Goat-anti-Mouse IgG IRDye™ 800 conjugated.

Western Blotting analysis of E. coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecopitates

Lane 1.
E.coli lysate containing DYKDDDDK-tagged fusion protein
Lane 2.
Immunoprecipitates of free E.coli lysate incubated with Anti-DYKDDDDK (Mouse IgG2b-κ) (2 µg/ml) and Protein A.
Lane 3
Immunoprecipitates of E.coli lysate containing DYKDDDDK-tagged fusion protein incubated with Anti-DYKDDDDK (Mouse IgG2b-κ) (2 µg/ml) and Protein A.

Immunoprecipitation: Anti-DYKDDDDK (Mouse), Agarose Conjugate

Immunoprecipitation-Western Blotting analysis of DYKDDDDK tagged fusion protein

Lane 1. DYKDDDDK tagged fusion protein input control
Lane 2. Anti-DYKDDDDK, Agarose Conjugate
Lane 3. DYKDDDDK tagged fusion protein was precipitated by Anti-DYKDDDDK, agarose conjugate.

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Anti-DYKDDDDK (Mouse IgG2a-k), Monoclonal (5A8E5) NU01102 -20 °C 200 µg 260.00 Buy
Anti-DYKDDDDK (Mouse), Monoclonal (6H8E4), Agarose Conjugate NU01103 -20 °C 1 ml 230.00 Buy