VECELL® 3D Cell Culture Dish
Cells are stretched out on conventional plastic plate and lose the native, in vivo structure without sufficient oxygen supply. Cells on porous membrane of gas permeable VECELL 3D cell culture plate shows more normal cell morphology and behavior.
After cell seeding 10 days
The survival rate after 10 days showed more than 80%. It was assayed by trypan blue staining. Most of rat hepatocytes were stable and alive for 10 days without any spheroid formation.
Cytochrome P450 activity
Cytochrome P450 activity of rat hepatocytes was maintained during even 10 days on VECELL 96-well Plate
Oxygen permeability from the bottom of culture plate
The oxygen tension at the well bottom of Gas-Permeable 3D Cell Culture Plate is almost the same as that at the atmosphere, 21.0 pO2(%).
Data courtesy of Prof. Sakai, Institute of Industrial Science, Tokyo University
VECELL membrane become transparent at wet condition.
Add 100 µl of culture medium to each well to allow the membrane surface to get wet and transparent, and then add sufficient volume of medium to each well (final volume should be up to 250 µl).
Cell seeding density should be from 20,000 to 80,000 cells/well depending on the cell line. Medium change should be done after 24 hours of seeding on VECELL Plate when the cell adhesion to the Plate becomes stable.
Avoid touching the membrane directly with pipette since the membrane is very thin (about 50 µl).
Cultured cells on VECELL Plate can be observed on a normal phase contrast microscope. A fluorescence microscope can also be used since the membrane does not contain any fluorescent substance.
For subculture, it is recommended to use normal trypsin treatment. The cell recovery rate is almost the same as the one on a normal plastic plate within 5 days of culture; however, the recovery rate may become lower when cultured longer than one week.
VECELL Plate cannot be used with organic solvent, including ethanol. Only aqueous medium should be used.