* Cannot be used with free fatty acids, sterol esters or sphingolipids.
This kit is suitable for measuring the fatty acid composition of glycerides. For other fatty acids (except sphingolipids), please use the regular Fatty Acid Methylation Kit, which can also methylate free fatty acids and sterol esters.
Methylation is done with a transesterification (methanolysis) reaction between the glyceride and methanol.
Reaction was confirmed by methylating 10 of a triglyceride (triolein) using the Fatty Acid Methylation Kit for Glycerides and performing TLC using a silica gel plate.
The triglyceride was successfully methylated.
In addition to saturated fatty acids, the sample contained large amounts of unsaturated fatty acids, such as DHA and EPA.
Methylation using the Fatty Acid Methylation Kit for Glycerides can be done quickly at room temperature, so volatile short-chain fatty acids, such as methyl acetate (4:0) and methyl hexanoate, can be analyzed.
Yeast - parent cell line 1103
Yeast - mutant cell line 0101 (cerulenin-resistant)
Data courtesy of Gakkeikan Sake Company, Ltd.
Main components: Galactolipids (glycolipids), phospholipids
Main components: Phosphatidylcholines, phosphatidylethanolamines
|Product Name||PKG Size||QTY|
|Solution A (Solvent)|
|Solution B (Reaction Solution)||10 ml||1|
|Solution C (Stop Solution)||100 ml||1|
|Fatty Acid Methylation Kit for glycerides(100 tests)||13246-84||Room Temp.||100 tests||294.00||Buy|
Because of hazardous materials and shipped directly from our manufacturing facility in Japan, the international shipping fee including hazardous material handling fee, about $230, will be charged. Otherwise, available from Sigma-Aldrich.