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COSMOSIL Diol-II: Gel Filtration Chromatography

Gel Filtration Chromatography

COSMOSIL Diol-120-II and Diol-300-II gel filtration columns are ideal for the size-based separation of proteins and other water soluble polymers. The separation MW range is 5,000-700,000 daltons for proteins and 300-300,000 daltons for water soluble polymers when Diol-120-II and Diol-300-II are used in series.

Features

  • Ideal for the size-based separation of proteins and water soluble polymers
  • Reduce undesirable adsorption
Packing Material5Diol-120-II5Diol-300-II
Silica Gel High Purity Porous Spherical Silica
Average Particle Size 5 µm
Average Pore Size approx. 120Å approx. 300Å
Stationary Phase Diol Group
Object Substance Protein and Water Soluble Polymer
Flow Rate 0.5-1.0 ml/min
Selection of Pore Size (Proteins) 5,000-100,000 10,000-700,000
Selection of Pore Size (Water Soluble Polymer) 300-30,000 500-300,000

 

Calibration Curve of Proteins

Condition

Column: COSMOSIL 5Diol-II 7.5mmI.D. x 600mm
Mobile phase: 20mmol/l Phosphate Buffer (pH 7.0)
+100mmol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30 °C
SampleMW   SampleMW
Thyroglobulin 660,000   Peroxidase 40,000
Catalase 250,000   Carbonic Anhydrase 30,000
Glucose Oxidase 160,000   α -Chymotrypsinogen A 25,700
Uricase 128,000   α -Chymotrypsin 25,200
Choline Oxidase 95,000   Trypsinogen 24,000
Transferrin 85,000   Trypsin (bovine) 23,300
Conalbumin 77,500   Myoglobin 17,000
Malate Dehydrogenase 70,000   Lysozyme 14,300
α -Glucosidase 68,500   Ribonuclease A 13,700
Albumin (BSA) 66,000   Cytochrome C 12,400
α -Amylase 52,500   Aprotinin 6,500
Fetuin 48,000   Gly-Gly 132
Albumin (Ovalbumin) 45,000      

Comparison with Competitors' Columns

COSMOSIL Diol-II series shows excellent separation compared to competitors' columns.

Column

Column Size

GelParticle
Size (avg.)
Pore Size
(avg.)
Chart
Speed
COSMOSIL 5Diol-300-II 7.5 mm I.D.
x 300 mm
Silica Gel 5 µm approx. 300Å 5 mm/min
TSKgel G3000 SWXL 7.8 mm I.D.
x 300 mm
Silica Gel 5 µm 250Å 5 mm/min
COSMOSIL 5Diol-120-II 7.5 mm I.D.
x 300 mm
Silica Gel 5 µm approx. 120Å 5 mm/min
TSKgel G2000 SWXL 7.8 mm I.D.
x 300 mm
Silica Gel 5 µm 125Å 5 mm/min

COSMOSIL 5Diol-300-II

COSMOSIL 5Diol-300-II, TSKgel G3000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Ferritin (Horse)
2. Peroxidase (Horse)
3. Cytochrome C (Horse)
4. Phenylglycine
 

COSMOSIL 5Diol-300-II

COSMOSIL 5Diol-300-II, TSKgel G3000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Transferrin (Human)
2. Hemoglobin (Human)
3. Angiotensin II (Human)
 

COSMOSIL 5Diol-120-II

COSMOSIL5Diol-120-II, TSKgel G2000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Sarcosine Dehydrogenase
2. Lysozyme
3. Phenylglycine
 

COSMOSIL 5Diol-120-II

COSMOSIL5Diol-120-II, TSKgel G2000 SWXL

Condition

Mobile phase: 0.01mol/l Na2HPO4+0.01mol/l
NaH2PO4+0.1mol/l Na2SO4
Flow rate: 1.0ml/min
Temperature: 30°C
Detection: UV220nm

Sample

1. Sarcosine Dehydrogenase
2. Lysozyme
3. Phenylglycine
 

Application Data

E. Coli Proteins

COSMOSIL Application Data

Sodium Polystyrene Sulfonate

COSMOSIL Application Data

Polyethylene Glycols

COSMOSIL Application Data

Pullulan

COSMOSIL Application Data

Human Serum

COSMOSIL Application Data

Ordering Information

COSMOSIL 5Diol-120-II
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL 5Diol-120-II Guard Column, 7.5 mm I.D. x 50 mm 38049-91 Waters type 1 pkg 470.00 Buy
COSMOSIL 5Diol-120-II Packed Column, 7.5 mm I.D. x 300 mm 38050-51 Waters type 1 pkg 1,000.00 Buy
COSMOSIL 5Diol-120-II Packed Column, 7.5 mm I.D. x 600 mm 38051-41 Waters type 1 pkg 1,510.00 Buy
COSMOSIL 5Diol-300-II
Product Cat.No. End-Fitting Quantity Price(US$)  
COSMOSIL 5Diol-300-II Guard Column, 7.5 mm I.D. x 50 mm 38052-31 Waters type 1 pkg 470.00 Buy
COSMOSIL 5Diol-300-II Packed Column, 7.5 mm I.D. x 300 mm 38053-21 Waters type 1 pkg 1,000.00 Buy
COSMOSIL 5Diol-300-II Packed Column, 7.5 mm I.D. x 600 mm 38054-11 Waters type 1 pkg 1,510.00 Buy