High-Performance iPS / ES Cell Culture Media for Basic and Clinical Research
StemFit®Basic02, Basic03, and Basic04 are capable of reprogramming, maintaining and differentiating human iPS and ES cells under feeder-free culture conditions.
Nakagawa, M. et al. (2014). A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific Reports 4, Article number: 3594.
http://www.nature.com/articles/srep03594?message-global=remove&WT.ec_id=SREP-20140114
Component |
Volume |
Storage |
Liquid A |
400 ml |
Store at below -20 °C |
Liquid B |
100 ml |
Store at below -20 °C |
Note: This product does not contain basic fibroblast growth factor (bFGF)
StemFit®Basic02 and Basic03 are provided frozen as a 2 component set containing “Liquid A” and “Liquid B” and can be stored at below -20 °C until use. Use sterile techniques to prepare StemFit® medium.
Cells : Human iPS cell line 201B7 (seeding density listed in each experiment)
Surface: iMatrix-511
Medium: StemFit®Basic02
Passage technique: Single cell passaging with cell scraping, 0.5x TrypLE Select/EDTA
StemFit®Basic02 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food. This product contains material derived from human plasma. Therefore, this product should be treated as potentially infectious agents and in accordance with universal handling procedures.
Product | Cat.No. | Storage | Quantity | Price(US$) | |
---|---|---|---|---|---|
StemFit Basic02 | BASIC02 | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 300.00 | Buy |
StemFit Basic03 | BASIC03 | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 600.00 | Buy |
StemFit Basic03 (6 sets) | BASIC03-6 | -20°C | Liquid A: 400 mL / Liquid B: 100 mL | 3,480.00 | Buy |
StemFit Basic04 | BASIC04 | -20°C | 500 mL | 300.00 | Buy |
StemFit Basic04 (6 sets) | BASIC04-6 | -20°C | 500 mL | 1,710.00 | Buy |